Characterization of Lymphocyte Fibronectin

Hauzenberger, Dan; Martin, Nancy; Johansson, Staffan; Sundqvist, Kristina

doi:10.1006/excr.1996.0040

Cited by 8 publications

(6 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The analysis of the IIICS region showed that not all possible five splice variants were generated as described before. 25 By RT–PCR only three variants were generated, corresponding in size to V89, V64 and V0, whereas the other variants V120 and V95 were not detectable. By primers binding directly into the CS1 region a PCR product was obtained, which was identified as CS1 by cloning and sequencing.…”

Section: Discussionmentioning

confidence: 98%

See 1 more Smart Citation

Fibronectin synthesis by activated T lymphocytes: up‐regulation of asurface‐associated isoform with signalling function

et al. 2000

View full text Add to dashboard Cite

SUMMARYFibronectin (FN) is a major constituent of the extracellular matrix. We now provide evidence for a surface-associated isoform of FN that is synthesized by T cells upon activation. The T-cell-derived FN has an unusual splice pattern: an additional domain, EDB, is produced whereas sequences within another domain, IIICS, are spliced out. CS1, the binding domain for very late antigen-4 (VLA-4), however, is still generated. To study the potential function of surface-associated FN its synthesis was down-regulated by an antisense oligonucleotide, then proliferation of T cells was induced by cross-linked anti-CD3. Proliferation was reduced as was expression of CD25. Moreover, when T cells were cultured in high density, the synthetic peptide QILDVPST, corresponding to CS1, inhibited proliferation, as did antibodies to VLA-4. We propose that surface-associated FN is a ligand for VLA-4, which by binding to VLA-4 on an adjacent cell, provides a costimulatory signal, thus sustaining T-cell proliferation.

show abstract

Section: Discussionmentioning

confidence: 98%

“…T cells are also able to synthesize FN, described firstly as a lymphokine‐like molecule, participating in cell–cell interaction. 23,24 More recently, presence of FN also on the T‐cell surface was reported, 25 whose function, however, remains elusive as yet.…”

Section: Introductionmentioning

confidence: 99%

Fibronectin synthesis by activated T lymphocytes: up‐regulation of asurface‐associated isoform with signalling function

et al. 2000

View full text Add to dashboard Cite

show abstract

“…Further, a previous study reported the lack of expression of the IIICS domain in lymphocytes that frequently contaminate FNA samples (21). Thus, the contamination of fibroblasts in FNABs can be a cause of false positive results in ABRP analysis, because a FNAB sample in which onfFN mRNA is detected by RT-PCR might be diagnosed as papillary or anaplastic carcinoma.…”

Section: Discussionmentioning

confidence: 98%

Expression of Oncofetal Fibronectin Messenger Ribonucleic Acid in Fibroblasts in the Thyroid: A Possible Cause of False Positive Results in Molecular-Based Diagnosis of Thyroid Carcinomas¹

Takano

Miyauchi

Matsuzuka

et al. 2000

The Journal of Clinical Endocrinology & Metabolism

View full text Add to dashboard Cite

Oncofetal fibronectin (onfFN) messenger ribonucleic acid (mRNA) is abundantly expressed in thyroid papillary and anaplastic carcinomas. These carcinomas can be preoperatively diagnosed by the detection of onfFN mRNA in fine needle aspiration biopsies (FNABs). However, previous reports have noted that the expression of onfFN mRNA was observed in 3.7% of the FNABs that were diagnosed as negative cytology. To clarify this discrepancy, we examined the expression of onfFN mRNA in fibroblasts in the thyroid. By RT-PCR and real-time quantitative RT-PCR analyses, we detected a high copy number of onfFN mRNA in cultured fibroblasts obtained from the normal thyroid tissues dissected surgically. Thus, we conclude that the contaminated fibroblasts in FNABs due to tumor necrosis or acute or chronic inflammation may be a cause of false positive results in molecular-based diagnosis of thyroid carcinomas. (J Clin Endocrinol Metab 85: [765][766][767][768] 2000)

show abstract

“…Fibronectin on the surface of lymphocytes can be both of endogenous and exogenous origin, because it is synthesized in lymphocytes and is affinitive to the receptors and on their surface (Hauzenberger, 1996). This glycoprotein is involved in the reaction of inter-cellular interaction, migration of lymphocytes in the fibrous tissue, processes of their activation and proliferation and directly affects on the course of inflammatory process (Schreiber et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

FN1 mRNA expression of fibronectin 1 and distribution of fibronectin-associated leukocytes in humans with chronic diffuse liver diseases

Хомич

Usenko

Дышлюк

2020

Regul. Mech. Biosyst.

View full text Add to dashboard Cite

Chronic diffuse liver diseases are characterized by continuous progression of fibrosis, ultimately leading to cirrhosis with the following loss of the normal functioning of this organ due to excessive accumulation of the components of extracellular matrix. To find new, more available diagnostic markers of detecting disorders in the liver, we used methods of antifungal cytofluorometry and quantitative real-time polymerase chain reaction. Intensity of exposure of fibronectin and plasmatic membrane of lymphocytes in the group of patients with chronic diffuse diseases compared with the control group of practically healthy donors decreased both inside and on the surface of the cells respectively by 45.3% and 16.2%. Similar tendency towards decrease was observed during the assays of the level of the exposure of fibronectin on the surface and inside the blood granulocytes: by 25.0% and 36.5%, respectively. In the blood of the patients suffering from chronic diffuse diseases, compared with the control group, there was determined reliable increase in percentage of lymphocytes and granulocytes which contain topical fibronectin, by 32.3% and 2.78 times, correspondingly. The level of monocytes (as a percentage) with cell-associated fibronectin and fibronectin localized inside, by contrast, reliably decreased in 2.07 and 4.50 times, respectively. Analysis of the expression of FN1 in lymphocytes of blood of the studied groups using quantitative real-time polymerase chain reaction revealed decrease in the level of FN1 mRNA expression by 34.0% in the group of ill patients compared with the control group. We determined excellent diagnostic informativeness of the parameters of the level of exposure of fibronectin inside and on the surface of granulocytes and prognostic accuracy of the classifier from these parameters at the level of 100% using the method of support vector machine, SVM. High levels of diagnostic informativeness were recorded for the tests of all types of analyzed leukocytes with cell-associated fibronectin, and the classifiers based on the pair combinations of the tests with cell-associated fibronectin and fibronectin localized within the cells provide high diagnostic accuracy of the prognosis. Because the mentioned indicators are highly-sensitive tests, they can be proposed for early diagnostics and evaluation of the effectiveness of the conducted therapy of chronic diffuse liver diseases, which would allow reducing the use of paracentetic trepanobiopsy, a painful and risky procedure, which still remains the main type of diagnostic.

show abstract

Characterization of Lymphocyte Fibronectin

Cited by 8 publications

References 0 publications

Fibronectin synthesis by activated T lymphocytes: up‐regulation of asurface‐associated isoform with signalling function

Fibronectin synthesis by activated T lymphocytes: up‐regulation of asurface‐associated isoform with signalling function

Expression of Oncofetal Fibronectin Messenger Ribonucleic Acid in Fibroblasts in the Thyroid: A Possible Cause of False Positive Results in Molecular-Based Diagnosis of Thyroid Carcinomas¹

FN1 mRNA expression of fibronectin 1 and distribution of fibronectin-associated leukocytes in humans with chronic diffuse liver diseases

Contact Info

Product

Resources

About

Characterization of Lymphocyte Fibronectin

Cited by 8 publications

References 0 publications

Fibronectin synthesis by activated T lymphocytes: up‐regulation of asurface‐associated isoform with signalling function

Fibronectin synthesis by activated T lymphocytes: up‐regulation of asurface‐associated isoform with signalling function

Expression of Oncofetal Fibronectin Messenger Ribonucleic Acid in Fibroblasts in the Thyroid: A Possible Cause of False Positive Results in Molecular-Based Diagnosis of Thyroid Carcinomas1

FN1 mRNA expression of fibronectin 1 and distribution of fibronectin-associated leukocytes in humans with chronic diffuse liver diseases

Contact Info

Product

Resources

About

Expression of Oncofetal Fibronectin Messenger Ribonucleic Acid in Fibroblasts in the Thyroid: A Possible Cause of False Positive Results in Molecular-Based Diagnosis of Thyroid Carcinomas¹