Characterization of Major Structural Proteins of Measles Virus with Monoclonal Antibodies

Abstract

SUMMARYWe have prepared and characterized monoclonal antibodies against five major structural proteins, i.e. the HA, P, NP, F and M proteins, of measles virus. At least three non-overlapping antigenic sites were delineated on the HA protein, three on the P, four on the NP, four on the F and five on the M proteins by competitive binding assays. Antigenic sites on the HA and F proteins roughly represented functional domains defined by serological tests. The reactivity of monoclonal antibodies with various measles virus strains including those from subacute sclerosing panencephalitis (SSPE) and other members of the morbillivirus family was studied by immunofluorescence. A monoclonal antibody or set of monoclonal antibodies to each of the antigenic sites showed a characteristic pattern of cross-reactivity with heterologous strains. The HA and NP proteins were antigenically the most variable, followed by the F and M proteins, while the P protein was relatively stable. None of the 14 anti-M monoclonal antibodies reacted with non-virus-producing SSPE cells, strongly suggesting the absence of M protein in these cells.

Antigenic Characterization of the Nucleoprotein and Matrix Protein of Influenza C Virus with Monoclonal Antibodies

“…Bucher et al (1989) analysed the reactivity of 19 anti-Ml MAbs with a broad panel of influenza A viruses and showed that many of the antibodies exhibited marked differences in reactivity among different strains. The occurrence of antigenic changes was also observed in the NP and M proteins of paramyxoviruses including measles virus (Sheshberadaran et al, 1983;Sato et al, 1985), mumps virus (Rydbeck et al, 1986) and Newcastle disease virus (Nishikawa et al, 1987). Thus the influenza C virus NP and M proteins may be the least variable among the corresponding proteins of orthomyxo-and paramyxoviruses examined so far.…”

Increased binding activity of measles virus to monkey red blood cells after long-term passage in Vero cell cultures

“…Unfixed plaques on MV-infected Vero cell monolayers were incubated with monoclonal antibody (MAb) against the H protein of the Toyoshima strain of MV (B5) (Kohama et al, 1985;Sato et al, 1985). After incubation for 1 h, the cells were washed three times with PBS, and incubated with fluorescein isothiocyanate (FITC)-conjugated anti-mouse IgG for 1 h. The cells were washed three times again with PBS and observed under a fluorescent microscope.…”

Purification and Characterization of Chick Interferon Induced by Viruses

“…with CE IFN for hybridoma production (Sato et al, 1985). This monoclonal antibody completely neutralized the crude CE IFN and both the 18K and 20K to 30K IFNs-( Table 2), implying that the CE IFN induced by UV-NDV contains an antigenically single IFN molecule, It has been reported that mouse L cells produce both c~ and/3 IFNs after induction with NDV (Yamamoto & Kawade, 1980).…”