Characterization of Maltase and Sucrase Inhibitory Constituents from Rhodiola crenulata

Li, Wen‐Tai; Chuang, Yen; Hsieh, Jung-Feng

doi:10.3390/foods8110540

Cited by 12 publications

(14 citation statements)

References 28 publications

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“…The MANE extract displays significant maltase and sucrase inhibition and is one of the most effective reported to date. The observed variation in IC 50 reported in the literature could be linked to the use of specific experimental conditions, seaweed species, extraction procedures, extract composition and bioactive component physicochemical properties ( 45 , 49 , 50 ) .…”

Section: Discussionmentioning

confidence: 99%

Comparison of edible brown algae extracts for the inhibition of intestinal carbohydrate digestive enzymes involved in glucose release from the diet

et al. 2021

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Type II diabetes is considered the most common metabolic disorder in the developed world and currently affects about one in ten globally. A therapeutic target for the management of type II diabetes is the inhibition of α- glucosidase, an essential enzyme located at the brush border of the small intestinal epithelium. The inhibition of α-glucosidase results in reduced digestion of carbohydrates and a decrease in postprandial blood glucose. Although pharmaceutical synthetic inhibitors are available, these are usually associated with significant gastrointestinal side effects. In the present study, the impact of inhibitors derived from edible brown algae is being investigated and compared for their effect on glycaemic control. Carbohydrate- and polyphenolic-enriched extracts derived from Ascophyllum nodosum, Fucus vesiculosus and Undaria pinnatifida were characterised and screened for their inhibitory effects on maltase and sucrase enzymes. Furthermore, enzyme kinetics and the mechanism of inhibition of maltase and sucrase were determined using linear and nonlinear regression methods. All tested extracts showed a dose-dependent inhibitory effect of α-glucosidase with IC50 values ranging from 0⋅26 to 0⋅47 mg/ml for maltase; however, the only extract that was able to inhibit sucrase activity was A. nodosum, with an IC50 value of 0⋅83 mg/ml. The present study demonstrates the mechanisms in which different brown seaweed extracts with varying composition and molecular weight distribution differentially inhibit α-glucosidase activities. The data highlight that all brown seaweed extracts are not equal in the inhibition of carbohydrate digestive enzymes involved in postprandial glycaemia.

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Section: Discussionmentioning

confidence: 99%

Comparison of edible brown algae extracts for the inhibition of intestinal carbohydrate digestive enzymes involved in glucose release from the diet

et al. 2021

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“…The sucrase inhibition assay was performed according to the method outlined by Honda et al with some modifications [33]. In brief, 10 µL of enzyme solution (100 U/mL) and 30 µL of test sample solution with appropriate concentration (dissolved in distilled water) were added into the 96-well plates, and were incubated for 10 min at 37 • C. The reaction was conducted by addition of 100 µL of sucrose solution (60 mM, dissolved in 0.1 M phosphate buffer solution).…”

Section: Sucrase Inhibition Assaymentioning

confidence: 99%

Acylated Aminooligosaccharides from the Yellow Sea Streptomyces sp. HO1518 as Both α-Glucosidase and Lipase Inhibitors

Liu

et al. 2020

Marine Drugs

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Three new acylated aminooligosaccharide (1–3), along with five known congeners (4–8), were isolated from the marine-derived Streptomyces sp. HO1518. Their structures were fully elucidated by extensive spectroscopic analysis, mainly based on 1D-selective and 2D TOCSY, HSQC-TOCSY, and HRESIMS spectrometry measurements, and by chemical transformations. All of the compounds were evaluated for their α-glucosidase and pancreatic lipase inhibitory activities. Among the isolates, D6-O-isobutyryl-acarviostatin II03 (3) and D6-O-acetyl-acarviostatin II03 (8), sharing acarviostatin II03-type structure, showed the most potent α-glucosidase and lipase inhibitory effects, far stronger than the antidiabetic acarbose towards α-glucosidase and almost equal to the anti-obesity orlistat towards lipase in vitro. This is the first report on inhibitory activities against the two major digestive enzymes for acylated aminooligosaccharides. The results from our investigation highlight the potential of acylated aminooligosaccharides for the future development of multi-target anti-diabetic drug.

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“…Rhodiola is inclined to grow in mountainous areas of low temperature such as precipices, tundra, riverbanks, and rock ledges in the northern hemisphere including Asia, North and Central Europe, and North America [3]. It consists of more than 100 species, of which about 20 species are used as traditional medicines, including Rhodiola rosea (R. rosea), Rhodiola crenulata (R. crenulata), Rhodiola sacra (R. sacra), and Rhodiola kirilovii [4,5].…”

Section: Introductionmentioning

confidence: 99%

“…crenulata ), Rhodiola sacra ( R . sacra ), and Rhodiola kirilovii [ 4 , 5 ]. Growing studies have demonstrated that Rhodiola possesses varieties of bioactivities such as antistress, antifatigue, antioxidant, antitumor, anti‐inflammation, antiaging, antiradiation, and immunomodulatory [ 2 , 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Quality Evaluation of Randomized Controlled Trials of Rhodiola Species: A Systematic Review

Chen

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Background. Rhodiola is a worldwide used medicinal plant for its various medicinal functions, and the number of randomized controlled trials (RCTs) of Rhodiola is increasing in recent years. This study aims to evaluate the reporting quality and risk of bias of the current RCT reports of different Rhodiola species. Methods. Six databases including Embase, PubMed, Web of Science, the Cochrane Library, ClinicalTrial.gov, and China National Knowledge Infrastructure were searched to identify RCTs that used Rhodiola as a single intervention and were published in English or Chinese from inception to December 2020. The Consolidated Standards of Reporting Trials (CONSORT) 2010 statement was used as the checklist for assessment, and a scoring system was applied to the evaluation of RCTs. Score 0 represents no reporting or inadequate reporting, and score 1 represents adequate reporting. The risk of bias of the included studies was also assessed using the Cochrane Risk of Bias tool. Results. A total of 39 RCTs were included in this study, including 23 RCTs of Rhodiola rosea (R. rosea), 8 RCTs of Rhodiola crenulata (R. crenulata), and 8 RCTs of Rhodiola wallichiana (R. wallichiana). None of the included studies met all the CONSORT statement criteria, and the reporting quality of RCTs of the three Rhodiola species was all generally poor. Based on the risk of bias assessment, the majority of included studies were judged to have an unclear risk of bias in most domains due to inadequate reporting. Conclusions. There is inadequate reporting among the included RCTs of different Rhodiola species, and RCTs of Rhodiola with higher reporting quality and better methodological quality are needed.

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Characterization of Maltase and Sucrase Inhibitory Constituents from Rhodiola crenulata

Cited by 12 publications

References 28 publications

Comparison of edible brown algae extracts for the inhibition of intestinal carbohydrate digestive enzymes involved in glucose release from the diet

Comparison of edible brown algae extracts for the inhibition of intestinal carbohydrate digestive enzymes involved in glucose release from the diet

Acylated Aminooligosaccharides from the Yellow Sea Streptomyces sp. HO1518 as Both α-Glucosidase and Lipase Inhibitors

Quality Evaluation of Randomized Controlled Trials of Rhodiola Species: A Systematic Review

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