Characterization of mandarin citrus germplasm from Southern Brazil by morphological and molecular analyses

Abstract

-The objective of this work was to characterize mandarin (Citrus spp.) germplasm from Southern Brazil by morphological and molecular analyses. Thirty seven cultivars from 34 distinct mandarin varieties were evaluated by morphological and agronomic traits of leaves, flowers and fruits, and by microsatellite markers. The morphological and agronomic characteristics suggested that almost all varieties can be produced for commercial use, and some, as the Satsuma variety, are recommended for breeding programs. Pooled DNA samples from 1-5 plants belonging to each cultivar were tested. Eight of the nine primers detected polymorphisms. Specific markers were found for some accessions. The dendrogram constructed with the morphological results divided the 37 cultivars into four groups, while that obtained with the microsatellites clustered 35 of the 37 cultivars into three groups only. Generally, intervarietal differences are not high, and this lack of agreement in the two multifactorial analyses indicates that diverse evolutionary factors are acting at these two levels of investigation.Index terms: citrus, genetic variation, microsatellites, breeding methods. Caracterização do germoplasma de tangerineiras do Sul do Brasil mediante análises morfológicas e molecularesResumo -O objetivo deste trabalho foi caracterizar o germoplasma de tangerineiras (Citrus spp.) por meio de análises morfológicas e moleculares. Trinta e sete cultivares, pertencentes a 34 variedades distintas de tangerineiras, foram avaliadas pelas características morfológicas e agronômicas de folhas, flores e frutos, bem como por análises de microssatélites. As características morfológicas e agronômicas sugerem que praticamente todas as variedades podem ser usadas comercialmente e algumas delas, como a variedade Satsuma, são recomendadas para uso em cruzamentos dirigidos. O DNA foi obtido de amostras extraídas de uma a cinco plantas de cada cultivar. Oito de nove primers detectaram polimorfismos. Foram encontrados marcadores específicos para alguns acessos. O dendrograma construído com os resultados morfológicos dividiu as 37 cultivares em quatro grupos, enquanto o obtido com os microssatélites agrupou 35 das 37 cultivares em três grupos. As diferenças intervarietais não são grandes, e esta falta de concordância nas duas análises multivariadas indica que fatores evolutivos diversos estão agindo nesses dois níveis de investigação.Termos para indexação: citros, variação genética, microsatélites, método de melhoramento.

Phenotypic and molecular selection of yellow passion fruit progenies in the second cycle of recurrent selection

“…On the other hand, the agronomic x molecular data showed were not significantly correlated (-0.02). Similar results were observed in other crops, e.g., oat (Moser and Lee 1994) and citrus (Koehler-Santos et al 2003).…”

Development and characterization of polymorphic microsatellite markers for the sweet orange (Citrus sinensis L. Osbeck)

“…It is interesting to note that the level of conservation of the microsatellite loci developed in our study has also been observed in studies on grapefruit and mandarin (Corazza-Nunes et al, 2002;Koehler-Santos et al, 2003), in work on the saturation of genetic maps (Cristofani et al, 2003) and in the multiple analyses of the loci of inter-specific progenies (Cristofani et al, 2001;Oliveira et al, 2002). For this reason it is important that the microsatellite markers developed by us are made available for studies involving the molecular characterization of different citrus species and hybrids, to which end we have fully listed them on the CAPTACSM website site (www.centrodecitricultura.br/SSR.htm).…”

“…Printed in Brazil www.sbg.org.br (Viruel and Hormaza, 2004). In Citrus and related genera, microsatellites have been employed for phylogenetic studies (Fang and Roose, 1997;Pang et al, 2003), assessment of genetic variability (Kijas et al, 1995;Corazza-Nunes et al, 2002;Koehler-Santos et al, 2003), identification of zygotic plants (Ruiz et al, 2000;Cristofani et al, 2001;Oliveira et al, 2002) and construction and integration of linkage maps (Kijas et al, 1997;Cristofani et al, 2003) but it is still difficult to identify intraspecific variability in some Citrus species.…”

Caracterização agronômica e molecular de genótipos diplóides melhorados de bananeira

“…Um total de 16 pares de primers foram utilizados, sendo seis pertencentes à série Ma, desenvolvida por Crouch et al (1998); cinco pares da série AGMI, desenvolvidos por Lagoda et al (1998); três da série MaOCEN, obtidos por Creste et al (2006); um primer (STMS 1), obtido por Kaemmer et al (1997), e um primer (Mb 1-100), descrito por Oriero et al (2006) (Tabela 2).…”