Characterization of mandarin fish (Siniperca chuatsi) IL-6 and IL-6 signal transducer and the association between their SNPs and resistance to ISKNV disease

Jin, Rui-Ming; Huang, He-Zhong; Zhou, Yu; Wang, Yingying; Fu, Huang-Cui; Li, Ze; Fu, Xiaozhe; Li, Ningqiu

doi:10.1016/j.fsi.2021.04.003

Cited by 24 publications

(4 citation statements)

References 33 publications

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“…We identified 2 overlapping genomic regions on chromosome Z of experimental ducks, which were chosen as candidate selection regions; these regions included genes associated with immune response ( IL7R and IL6ST ). As multifunctional cytokines, IL-6 and IL-7 exert biological functions mainly via activation of their receptors (IL6ST and IL7R), which play an essential role in the signaling and coordination of innate and acquired immune responses ( Jin et al, 2021 ; Wang et al, 2022 ). Notably, the genotyping of IL7R and IL6ST in the 3 experimental duck populations largely converged, possibly because in this study, experimental ducks underwent rigorous decontamination during egg selection, hatching, and rearing, along with whole-flock screening for pathogens and elimination of positive individuals, which consequently ensured that no infectious agents were present.…”

Section: Discussionmentioning

confidence: 99%

Whole-genome resequencing to unveil genetic characteristics and selection signatures of specific pathogen-free ducks

Li¹,

Quan²,

Gao³

et al. 2023

Poultry Science

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Section: Discussionmentioning

confidence: 99%

Whole-genome resequencing to unveil genetic characteristics and selection signatures of specific pathogen-free ducks

Li¹,

Quan²,

Gao³

et al. 2023

Poultry Science

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“…IL-6 forms a complex with IL-6R, which binds to the membrane protein gp130, which activates intracellular signal transduction to function. It can promote the proliferation activation of T cells and the expression of IL-2 receptor on the surface of T cells, which further assists the proliferation and differentiation of B cells and the production of antibodies to participate in the humoral immune response ( 89 , 90 ). A previous study showed that cyclosporine A (CsA) inhibited T lymphocyte expression by blocking activation of the transcription factor NFAT, and then inhibited B lymphocyte expression and antibody production.…”

Section: Discussionmentioning

confidence: 99%

Splenic protection network revealed by transcriptome analysis in inactivated vaccine-immunized flounder (Paralichthys olivaceus) against Edwardsiella tarda infection

Xing²,

Tang³

et al. 2022

Front. Immunol.

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The protective immune response produced by fish after vaccination is crucial for vaccine effectiveness. Our previous studies have shown inactivated vaccine against Edwardsiella tarda can induce immune response in flounder (Paralichthys olivaceus). To elucidate the protective immune response at the genetic level, in this study, flounder was immunized with inactivated E. tarda for 5 weeks, and then they were challenged with E. tarda. The spleen was dissected at 7th day post immunization, 1st and 7th day post challenge, respectively. Transcriptome analysis showed that average of 46 million clean reads were obtained per library, while percentage of clean reads being mapped to reference genome was more than 89% in all cases, which suggested good quality of samples. As for differentially expressed genes (DEGs) identification in inactivated E. tarda groups, at 7th day post immunization, 1422 DEGs were identified and significantly enriched in innate immune-related pathways, such as Phagosome, Cell adhesion molecules and NF-kappa B signaling pathway; At 1st post challenge day, 1210 DEGs were identified and enriched to Antigen processing and presentation and Cell adhesion molecules, indicating that the pathogen was rapidly recognized and delivered; At 7th post challenge day, 1929 DEGs were identified, belonged to Toll-like receptor signaling pathway, Antigen processing and presentation, Th1 and Th2 cell differentiation and Th17 cell differentiation. Compared to 7th post immunization day, 73 immune-associated DEGs were identified at 1st post challenge day. Protein-protein interaction networks analysis revealed 11 hub genes (TLR7, TLR3, CXCR4, IFIH1, TLR8 etc), associated with recognition of pathogens and activation of innate immunity; while for 7th post challenge day, 141 immune-associated DEGs were identified. 30 hub genes (IL6, STAT1, HSP90A.1, TLR7, IL12β etc) were associated with stimulation of lymphocyte differentiation and activation of cellular immunity. Ten immune-related genes were randomly selected for RT-qPCR validation at each time point. In conclusion, data revealed protection of flounder against E. tarda infection by inactivated vaccine is mediated via immediate recognition of pathogen and subsequently activation of cellular immunity. Results give new aspect for vaccine protection cascades, is good references for vaccine evaluation.

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“…The presence of homologous genes or sequences initially complicates allele identification, however the simplicity with which SNPs can be scored promotes allele discrimination 6 . SNP markers have been widely employed in fish disease resistance 7 , 8 , feed conversion efficiency 9 , 10 , growth rate 11 , 12 , muscle yield 13 , reproductive characteristics 14 , 15 and tolerance to environmental stressors 16 , 17 since the emergence of high-throughput sequencing technology. Similarly, SNP marker-related sequencing data increased rapidly.…”

Section: Background and Summarymentioning

confidence: 99%

FishSNP: a high quality cross-species SNP database of fishes

Zhang,

Li,

Shi

et al. 2024

Sci Data

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The progress of aquaculture heavily depends on the efficient utilization of diverse genetic resources to enhance production efficiency and maximize profitability. Single nucleotide polymorphisms (SNPs) have been widely used in the study of aquaculture genomics, genetics, and breeding research since they are the most prevalent molecular markers on the genome. Currently, a large number of SNP markers from cultured fish species are scattered in individual studies, making querying complicated and data reuse problematic. We compiled relevant SNP data from literature and public databases to create a fish SNP database, FishSNP (http://bioinfo.ihb.ac.cn/fishsnp), and also used a unified analysis pipeline to process raw data that the author of the literature did not perform SNP calling on to obtain SNPs with high reliability. This database presently contains 45,690,243 (45 million) nonredundant SNP data for 13 fish species, with 30,288,958 (30 million) of those being high-quality SNPs. The main function of FishSNP is to search, browse, annotate and download SNPs, which provide researchers various and comprehensive associated information.

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Characterization of mandarin fish (Siniperca chuatsi) IL-6 and IL-6 signal transducer and the association between their SNPs and resistance to ISKNV disease

Cited by 24 publications

References 33 publications

Whole-genome resequencing to unveil genetic characteristics and selection signatures of specific pathogen-free ducks

Whole-genome resequencing to unveil genetic characteristics and selection signatures of specific pathogen-free ducks

Splenic protection network revealed by transcriptome analysis in inactivated vaccine-immunized flounder (Paralichthys olivaceus) against Edwardsiella tarda infection

FishSNP: a high quality cross-species SNP database of fishes

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