Characterization of marine microbial communities around an Arctic seabed hydrocarbon seep at Scott Inlet, Baffin Bay

Cramm, Margaret; Neves, Bárbara de Moura; Manning, Cara C.; Oldenburg, Thomas B. P.; Archambault, Philippe; Chakraborty, Anirban; Cyr-Parent, Annie; Edinger, Evan; Jaggi, Aprami; Mort, Andrew J.; Tortell, Philippe D.; Hubert, Casey R.J.

doi:10.1016/j.scitotenv.2020.143961

Cited by 22 publications

(32 citation statements)

References 91 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As previously discussed (section 3.2, Figure 2d-e), CH4 concentrations in the Northwest Passage, Baffin Bay, and Davis Strait were generally low. Some seafloor seeps are present along the coast of Baffin Island (Cramm et al, 2021;Punshon et al, 2019). For example, as reported in Cramm et al (2021), we measured bottom water CH4 concentrations at the Scott Inlet methane seep ranging from 9 to 592 nmol kg −1 during five repeat measurements over 24 h in 2017, and video surveys with a remotely operated vehicle confirmed the presence of active ebullition from the seafloor at this site.…”

Section: Ch4 Distributions In the Northwest Passage Baffin Bay And Davis Straitsupporting

confidence: 74%

“…Some seafloor seeps are present along the coast of Baffin Island (Cramm et al, 2021;Punshon et al, 2019). For example, as reported in Cramm et al (2021), we measured bottom water CH4 concentrations at the Scott Inlet methane seep ranging from 9 to 592 nmol kg −1 during five repeat measurements over 24 h in 2017, and video surveys with a remotely operated vehicle confirmed the presence of active ebullition from the seafloor at this site. Punshon et al (2019) reported bottom-water CH4 concentrations of ~60 nmol kg −1 near Scott Inlet in 2012.…”

Section: Ch4 Distributions In the Northwest Passage Baffin Bay And Davis Straitsupporting

confidence: 74%

“…Data from stations located more than 200 km from the transect, as well as measurements collected directly over a hydrocarbon seep at Scott Inlet (northeastern coast of Baffin Island, 155 km from the transect), are not discussed in this manuscript. The Scott Inlet CH4 data are described in Cramm et al (2021). These off-transect stations (14 out of 129 stations, indicated by white triangles in Figure 1) were only sampled in one of the four years and cannot be used to investigate interannual variability in gas distributions.…”

Section: Research Expeditionsmentioning

confidence: 99%

See 2 more Smart Citations

Interannual variability in methane and nitrous oxide concentrations and sea-air fluxes across the North American Arctic Ocean (2015–2019)

Manning¹,

Zheng²,

Fenwick³

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

Between 2015–2018, we collected ~2000 measurements of methane (CH4) and nitrousoxide (N2O) concentrations in the North American Arctic Ocean during summer and early fall from water column profiles. We also obtained 25 measurements of CH4 and N2O concentrations in rivers along the Northwest Passage and Ellesmere Island in mid-summer 2017–2019. Our results show that N2O is generated in the highly productive Bering and Chukchi Seas and transported northeastward, producing a persistent subsurface N2O peak in the Beaufort Sea. The Chukchi and Beaufort Sea sediments are a significant source of CH4 to the water column. These sedimentary sources and associated water column consumption display significant spatial gradients and interannual variability. CH4 isotope data demonstrate the importance of CH4 oxidation across the study region. We find that rivers are not a significant source of CH4 or N2O to the Arctic Ocean at the time of year sampled. The estimated annual sea-air flux across the study region (2.3 million km2) had a median (interquartile range) of 0.009 (0.002, 0.023) Tg CH4 y-1 and −0.003 (−0.013, 0.010) Tg N y-1. These results suggest that the North American Arctic Ocean currently plays a negligible role in global CH4 and N2O budgets. Our expansive dataset, with observations at many repeat stations, provides a synopsis of present-day Arctic CH4 and N2O distributions and their range of variability, as well as a benchmark against which future climate-dependent changes can be evaluated.

show abstract

Section: Ch4 Distributions In the Northwest Passage Baffin Bay And Davis Straitsupporting

confidence: 74%

Section: Ch4 Distributions In the Northwest Passage Baffin Bay And Davis Straitsupporting

confidence: 74%

Section: Research Expeditionsmentioning

confidence: 99%

See 1 more Smart Citation

Interannual variability in methane and nitrous oxide concentrations and sea-air fluxes across the North American Arctic Ocean (2015–2019)

Manning¹,

Zheng²,

Fenwick³

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…no. 47014), following the manufacturer’s instructions with two modifications as previously published 59 . Bead beating was performed at 5.5 m s −1 for 45 s with a Bead Ruptor 24 (OMNI).…”

Section: Methodsmentioning

confidence: 99%

Genomic insights into diverse bacterial taxa that degrade extracellular DNA in marine sediments

et al. 2021

View full text Add to dashboard Cite

Extracellular DNA is a major macromolecule in global element cycles, and is a particularly crucial phosphorus, nitrogen and carbon source for microorganisms in the seafloor. Nevertheless, the identities, ecophysiology and genetic features of DNA-foraging microorganisms in marine sediments are largely unknown. Here, we combined microcosm experiments, DNA stable isotope probing (SIP), single-cell SIP using nano-scale secondary isotope mass spectrometry (NanoSIMS) and genome-centric metagenomics to study microbial catabolism of DNA and its subcomponents in marine sediments. 13C-DNA added to sediment microcosms was largely degraded within 10 d and mineralized to 13CO2. SIP probing of DNA revealed diverse ‘Candidatus Izemoplasma’, Lutibacter, Shewanella and Fusibacteraceae incorporated DNA-derived 13C-carbon. NanoSIMS confirmed incorporation of 13C into individual bacterial cells of Fusibacteraceae sorted from microcosms. Genomes of the 13C-labelled taxa all encoded enzymatic repertoires for catabolism of DNA or subcomponents of DNA. Comparative genomics indicated that diverse ‘Candidatus Izemoplasmatales’ (former Tenericutes) are exceptional because they encode multiple (up to five) predicted extracellular nucleases and are probably specialized DNA-degraders. Analyses of additional sediment metagenomes revealed extracellular nuclease genes are prevalent among Bacteroidota at diverse sites. Together, our results reveal the identities and functional properties of microorganisms that may contribute to the key ecosystem function of degrading and recycling DNA in the seabed.

show abstract

“…The Scott Inlet CH 4 data are described in Cramm et al. (2021). These off‐transect stations (14 out of 129 stations, indicated by white triangles in Figure 1) were only sampled in one of the four years and cannot be used to investigate interannual variability in gas distributions.…”

Section: Methods Data and Calculationsmentioning

confidence: 99%

Interannual Variability in Methane and Nitrous Oxide Concentrations and Sea‐Air Fluxes Across the North American Arctic Ocean (2015–2019)

Manning

Zheng

Fenwick

et al. 2022

Global Biogeochemical Cycles

View full text Add to dashboard Cite

Between 2015 and 2018, we collected approximately 2,000 water column measurements of methane (CH4) and nitrous oxide (N2O) concentrations in the North American Arctic Ocean during summer and early fall. We also obtained 25 measurements of CH4 and N2O concentrations in rivers along the Northwest Passage and Ellesmere Island in midsummer 2017–2019. Our results show that N2O is generated in the highly productive Bering and Chukchi Seas and transported northeastward, producing a persistent subsurface N2O peak in the Beaufort Sea. The Chukchi and Beaufort Sea sediments are a significant source of CH4 to the water column. These sedimentary sources and associated water column consumption display significant spatial gradients and interannual variability. CH4 isotope data demonstrate the importance of CH4 oxidation across the study region. We find that rivers are not a significant source of CH4 or N2O to the Arctic Ocean at the time of year sampled. The estimated annual sea‐air flux across the study region (2.3 million km2) had a median (first quartile, third quartile) of 0.009 (0.002, 0.023) Tg CH4 y−1 and −0.003 (−0.013, 0.010) Tg N y−1. These results suggest that the North American Arctic Ocean currently plays a negligible role in global CH4 and N2O budgets. Our expansive data set, with observations at many repeat stations, provides a synopsis of present‐day Arctic CH4 and N2O distributions and their range of variability, as well as a benchmark against which future climate‐dependent changes can be evaluated.

show abstract

Characterization of marine microbial communities around an Arctic seabed hydrocarbon seep at Scott Inlet, Baffin Bay

Cited by 22 publications

References 91 publications

Interannual variability in methane and nitrous oxide concentrations and sea-air fluxes across the North American Arctic Ocean (2015–2019)

Interannual variability in methane and nitrous oxide concentrations and sea-air fluxes across the North American Arctic Ocean (2015–2019)

Genomic insights into diverse bacterial taxa that degrade extracellular DNA in marine sediments

Interannual Variability in Methane and Nitrous Oxide Concentrations and Sea‐Air Fluxes Across the North American Arctic Ocean (2015–2019)

Contact Info

Product

Resources

About