Characterization of Mechanical Signature of Eutopic Endometrial Stromal Cells of Endometriosis Patients

Altayyeb, Ahmad; Othman, Essam R.; Khashbah, Maha Y.; Esmaeel, Abdelhady; El‐Mokhtar, Mohamed A.; Lambalk, Cornelis B.; Mijatovic, Velja; Abdelgawad, Mohamed

doi:10.1007/s43032-019-00042-3

Cited by 15 publications

(7 citation statements)

References 49 publications

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“…PHESCs were isolated from endometrial tissue biopsies collected from healthy non-pregnant women (n = 5), aged 27-35 years, admitted to Assiut University Hospitals, as described in our previous studies [30]. The isolated cells have a characteristic microscopic morphology that confirms that they were fibroblast-like cells ( Figure 1A).…”

Section: Isolation and Characterization Of Phescsmentioning

confidence: 81%

“…PHESCs were isolated from proliferative human endometrium collected from healthy non-pregnant women during the proliferative stage of menstruation (n = 5), aged 27-35 years without a history of gynecological abnormalities, as described previously [30] in Table 1. Briefly, endometrial biopsies were washed with phosphate-buffered saline (PBS), minced into 1-2-mm pieces and then digested by 0.1% collagenase type I for 2 h in a shaking water bath at 37 • C. PHESCs were purified and separated using 40 and 20 µm sieves filters.…”

Section: Isolation and Characterization Of Phescs From Non-pregnant Wmentioning

confidence: 99%

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Evidence of the Extrahepatic Replication of Hepatitis E Virus in Human Endometrial Stromal Cells

et al. 2020

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Hepatitis E virus (HEV) is the most common cause of acute viral hepatitis worldwide. The tropism of HEV is not restricted to the liver, and the virus replicates in other organs. Not all the extrahepatic targets for HEV are identified. Herein, we found that non-decidualized primary human endometrial stromal cells (PHESCs), which are precursors for the decidua and placenta, are susceptible to HEV infection. PHESCs, isolated from healthy non-pregnant women (n = 5), were challenged with stool-derived HEV-1 and HEV-3. HEV RNA was measured by qPCR, and HEV capsid protein was assessed by flow cytometry, immunofluorescence (IF), and ELISA. HEV infection was successfully established in PHESCs. Intracellular and extracellular HEV RNA loads were increased over time, indicating efficient replication in vitro. In addition, HEV capsid protein was detected intracellularly in the HEV-infected PHESCs and accumulated extracellularly over time, confirming the viral assembly and release from the infected cells. HEV-1 replicated more efficiently in PHESCs than HEV-3 and induced more inflammatory responses. Ribavirin (RBV) treatment abolished the replication of HEV in PHESCs. In conclusion, PHESCs are permissive to HEV infection and these cells could be an endogenous source of HEV infection during pregnancy and mediate HEV vertical transmission.

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Section: Isolation and Characterization Of Phescsmentioning

confidence: 81%

Section: Isolation and Characterization Of Phescs From Non-pregnant Wmentioning

confidence: 99%

Evidence of the Extrahepatic Replication of Hepatitis E Virus in Human Endometrial Stromal Cells

et al. 2020

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“…Among them, HESCs play an important role on endometrial metaplasia, vascular remodeling, immune cell recruitment, and abundant molecular production (Bozorgmehr et al, 2020). In the development of endometriosis, HESCs may play a role in infiltration, proliferation, and invasion, analogous to that of tumor cells (Altayyeb et al, 2020;Huang et al, 2020;Li et al, 2021). Therefore, controlling cell proliferation, migration, and invasion of HESCs may be an important breakthrough in the treatment of endometriosis (Gu and Zhou, 2021).…”

Section: Discussionmentioning

confidence: 99%

Moutan Cortex extract inhibited the proliferation and migration of endometrial stromal cells by inhibiting OPN-induced, MAPK-mediated MMP9 activation

Liu

Luo

et al. 2023

Qual. Assur. Saf. Crops Foods

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Endometriosis is frequent in women of childbearing age with a morbidity rate of approximately 10%. Its clinical features mainly manifest as dysmenorrhea, chronic pelvic pain, and infertility. However, effective treatments are still lacking; hence, it is necessary to identify an effective and safe treatment strategy on endometriosis. Moutan Cortex extract (MCE) was prepared by decoction. Then, cell proliferation and apoptosis in human endometrial stromal cells (HESCs) were detected by cell counting kit-8, EdU cell proliferation assay, and flow cytometry. Wound-healing assay and transwell migration assay were performed to explore cell migration or invasion. The expression of indicators of downstream signaling pathways was determined by Western blot analysis or enzyme-linked-immunosorbent serologic assay. MCE treatment inhibited cell viability and proliferation in HESCs while promoting cell apoptosis. MCE reduced migration and invasion of HESCs. Furthermore, MCE inhibited osteopontin-induced, mitogen-activated, protein kinase (MAPK)-mediated matrix metallopeptidase 9 (MMP9) activation, and upregulation of OPN reversed the effect of MCE on HESCs. In this study, MCE inhibited the proliferation and migration of endometrial stromal cells by inhibiting OPN induced, MAPK-mediated MMP9 activation. MCE might be a novel treatment strategy on endometriosis.

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“…They may also provide an inroad into the analysis of specimens that are confounded by pathologies, such as myoma, sarcoma, or uterine prolapse, that may affect tissue mechanics, as has been observed in endometriosis, an estrogen-dependent disorder that is characterized by the presence of endometrial tissue outside the uterus [125]. Eutopic endometrial stromal cells from healthy women are stiffer and have a lower deformation index than cells from endometriosis patients when placed inside a microchannel system [126].…”

Section: Discussionmentioning

confidence: 99%

How Mechanical Forces Change the Human Endometrium during the Menstrual Cycle in Preparation for Embryo Implantation

Sternberg

Buck

Claßen-Linke

et al. 2021

Cells

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The human endometrium is characterized by exceptional plasticity, as evidenced by rapid growth and differentiation during the menstrual cycle and fast tissue remodeling during early pregnancy. Past work has rarely addressed the role of cellular mechanics in these processes. It is becoming increasingly clear that sensing and responding to mechanical forces are as significant for cell behavior as biochemical signaling. Here, we provide an overview of experimental evidence and concepts that illustrate how mechanical forces influence endometrial cell behavior during the hormone-driven menstrual cycle and prepare the endometrium for embryo implantation. Given the fundamental species differences during implantation, we restrict the review to the human situation. Novel technologies and devices such as 3D multifrequency magnetic resonance elastography, atomic force microscopy, organ-on-a-chip microfluidic systems, stem-cell-derived organoid formation, and complex 3D co-culture systems have propelled the understanding how endometrial receptivity and blastocyst implantation are regulated in the human uterus. Accumulating evidence has shown that junctional adhesion, cytoskeletal rearrangement, and extracellular matrix stiffness affect the local force balance that regulates endometrial differentiation and blastocyst invasion. A focus of this review is on the hormonal regulation of endometrial epithelial cell mechanics. We discuss potential implications for embryo implantation.

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Characterization of Mechanical Signature of Eutopic Endometrial Stromal Cells of Endometriosis Patients

Cited by 15 publications

References 49 publications

Evidence of the Extrahepatic Replication of Hepatitis E Virus in Human Endometrial Stromal Cells

Evidence of the Extrahepatic Replication of Hepatitis E Virus in Human Endometrial Stromal Cells

Moutan Cortex extract inhibited the proliferation and migration of endometrial stromal cells by inhibiting OPN-induced, MAPK-mediated MMP9 activation

How Mechanical Forces Change the Human Endometrium during the Menstrual Cycle in Preparation for Embryo Implantation

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