Characterization of membrane-associated nuclease activity in Mycoplasma pulmonis

Abstract

Introduction 45 Materials and Methods 47 Organisms and media 47 Isolation of M. pulmonis chromosomal DNA Antisera Genomic libra^ 49 Library screening 49 Recombinant DNA Nuclease detection assays TnlOOO mutagenesis Hybridization smdies DNA sequence analysis Nucleotide sequence accession number Results Characterization of the anti-nuclease antisera Identification and characterization of immunoreactive X phage Cloning and functional analysis of the chromosomal fragment from phage 13-2B DNA sequencing and computer analysis Analysis of mnuA products produced in E. coli Presence of mnuA-like sequences in other mycoplasma species Discussion Acknowledgments CHAPTER 4. MOLECULAR ANALYSIS OF THE uvrB HOMOLOG IN