Characterization of metabolic profile of intact non-tumor and tumor breast cells by high-resolution magic angle spinning nuclear magnetic resonance spectroscopy

Maria, Roberta M.; Altei, Wanessa Fernanda; Andricopulo, Adriano D.; Becceneri, Amanda Blanque; Cominetti, Márcia Regina; Venâncio, Tiago; Colnago, Luiz Alberto

doi:10.1016/j.ab.2015.07.015

Cited by 23 publications

(31 citation statements)

References 28 publications

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“…Lactic acid and pyruvate, which were increased in serum, but decreased in tissues of the EC patients, revealed enhanced gluconeogenesis of EC cells, consistent with gastric cancer [24]. Lactic acid in serum and cancer tissues may indicate poor prognosis in many types of cancers [25–27]. In this study, most intermediates of the TCA cycle accumulated both in EC serum and tissues.…”

Section: Discussionsupporting

confidence: 56%

Metabolic Perturbation and Potential Markers in Patients with Esophageal Cancer

Zhu

Wang

Liu

et al. 2017

Gastroenterology Research and Practice

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Clinical diagnosis of esophageal cancer (EC) at early stage is rather difficult. This study aimed to profile the molecules in serum and tissue and identify potential biomarkers in patients with EC. A total of 64 volunteers were recruited, and 83 samples (24 EC serum samples, 21 serum controls, 19 paired EC tissues, and corresponding tumor-adjacent tissues) were analyzed. The gas chromatography time-of-flight mass spectrometry (GC/TOF-MS) was employed, and principal component analysis was used to reveal the discriminatory metabolites and identify the candidate markers of EC. A total of 41 in serum and 36 identified compounds in tissues were relevant to the malignant prognosis. A marked metabolic reprogramming of EC was observed, including enhanced anaerobic glycolysis and glutaminolysis, inhibited tricarboxylic acid (TCA) cycle, and altered lipid metabolism and amino acid turnover. Based on the potential markers of glucose, glutamic acid, lactic acid, and cholesterol, the receiver operating characteristic (ROC) curves indicated good diagnosis and prognosis of EC. EC patients showed distinct reprogrammed metabolism involved in glycolysis, TCA cycle, glutaminolysis, and fatty acid metabolism. The pivotal molecules in the metabolic pathways were suggested as the potential markers to facilitate the early diagnosis of human EC.

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Section: Discussionsupporting

confidence: 56%

Metabolic Perturbation and Potential Markers in Patients with Esophageal Cancer

Zhu

Wang

Liu

et al. 2017

Gastroenterology Research and Practice

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“…In the same study, the ratio between GPC and PCho was inverted from higher GPC/PCho to higher PCho/GPC with immortalization of cells, suggesting the latter ratio to be a marker for aggressiveness. Similar results were observed in a study comparing triple negative and triple positive cell lines by HR MAS MR spectroscopy of intact cells, where the triple negative cell line had higher PCho levels [93]. In contradiction, ex vivo studies have found GPC/PCho to be elevated in breast cancer subtypes with worse prognosis [47,75].…”

Section: Choline Phospholipid Metabolismsupporting

confidence: 76%

Metabolic Portraits of Breast Cancer by HR MAS MR Spectroscopy of Intact Tissue Samples

et al. 2017

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Despite progress in early detection and therapeutic strategies, breast cancer remains the second leading cause of cancer-related death among women globally. Due to the heterogeneity and complexity of tumor biology, breast cancer patients with similar diagnosis might have different prognosis and response to treatment. Thus, deeper understanding of individual tumor properties is necessary. Cancer cells must be able to convert nutrients to biomass while maintaining energy production, which requires reprogramming of central metabolic processes in the cells. This phenomenon is increasingly recognized as a potential target for treatment, but also as a source for biomarkers that can be used for prognosis, risk stratification and therapy monitoring. Magnetic resonance (MR) metabolomics is a widely used approach in translational research, aiming to identify clinically relevant metabolic biomarkers or generate novel understanding of the molecular biology in tumors. Ex vivo proton high-resolution magic angle spinning (HR MAS) MR spectroscopy is widely used to study central metabolic processes in a non-destructive manner. Here we review the current status for HR MAS MR spectroscopy findings in breast cancer in relation to glucose, amino acid and choline metabolism.

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“…HRMAS NMR was also used to investigate breast tumor cell lines MCF-7 and MDA-MB-231, along with the nontumor breast cell line MCF-10A, to evaluate cancer metabolism in intact cells. 107 Among the metabolites detected, phosphocholine exhibited higher concentrations in both tumor cells relative to controls and the level was higher for MDA-MB-231 than in MCF-7. Phosphocholine is recognized as a tumor marker for breast cancer.…”

Section: Applicationsmentioning

confidence: 90%