Characterization of metallo-β-lactamases-encoding genes blaIMP-1 and blaVIM-1 amongst Klebsiella pneumoniae from abattoir samples of Ebonyi state, southeastern Nigeria

Chika, Ejikeugwu; Saki, Morteza; Emmanuel, Nwakaeze; Eze, Peter M.; Chinedu, Orinya; Carissa, Duru; Edeh, Chijioke; Charles, Esimone; Ifeanyichukwu, Iroha; Adikwu, M. U.

doi:10.1016/j.genrep.2019.100428

Cited by 7 publications

(4 citation statements)

References 9 publications

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“… Paenalcaligenes spp. 119/135 (88.1) NA NA [ 108 ] 2016 Hospital environment (sinks, beddings, equipment, furniture and walls) P. aeruginosa 2/20 (10) NA NA [ 109 ] 2016 Anal swabs of cow K. pneumoniae 46/59 (78) bla IMP-1 NA [ 110 ] 2016–2019 Pigs, poultry, hospital environment, cattle, camel, dogs E. coli, K. pneumoniae 77/488 (15.8) bla SHV-11 , bla SHV-28 NA [ 111 ] 2016–2019 Rectal/cloacae swabs from camels, cattle, dogs, pigs and poultry Enterobacteriaceae, Alcaligenes faecalis NA bla SHV-11 , bla TEM-93 , bla TEM-57 NA [ 112 ] Nigeria 2017 Water, soil Enterobacteriaceae A. baumannii, Pseudomonas spp. 259 bla POM-1 , bla CGB -like, bla PenA , bla OXA-181 , bla OXA-23 , bla OXA-40 , bla NDM-1 , bla NDM-5 , bla NDM-7 , bla VIM-5 NA [ 113 ] 2017 Cattle and Poultry Faeces, poultry litter …”

Section: Resultsmentioning

confidence: 99%

Carbapenem Resistance in Animal-Environment-Food from Africa: A Systematic Review, Recommendations and Perspectives

Dossouvi,

Ametepe

2024

IDR

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Background The World Health Organization (WHO) has classified carbapenem-resistant Enterobacteriaceae, Pseudomonas aeruginosa ( P. aeruginosa ), and Acinetobacter baumannii ( A. baumannii ) as high-priority pathogens, and carbapenem-resistant bacteria (CRB) have been reported to spread between humans, animals, and the environment. Objective This study aimed to conduct a systematic review of carbapenem resistance in animals, foods, and the environment on the African continent and to provide recommendations and perspectives for better prevention and control of carbapenem resistance in Africa. Results A total of 137 research articles collected from 2009 to 2023 were selected for this review, including articles reporting carbapenem-resistant bacteria in animals (81/137; 59.1%), the environment (66/137; 48.2%), and foods (26/137; 19%). Carbapenem-resistant bacterial species belonged to 31 genera and 17 families, including mainly Escherichia spp. (68/127; 53.5%); Klebsiella spp. (45/127; 35.4%); Pseudomonas spp. (20/127; 15.7%), Enterobacter spp. (19/127; 15%) and Acinetobacter spp. (15/127; 11.8%). The prevalence of CRBs by country ranged from 1.1% to 48.5%, and the pooled prevalence of CRBs isolated from animal-environment-food in Africa was 19.1% (2804/14,684; Standard Deviation = 15). Twenty carbapenemase families belonging to A, B, C, and D Ambler classes were reported, including mainly carbapenemase genes from bla OXA (44/84; 52.4%), bla NDM (34/84; 40.5%), bla SHV (23/84; 27.4%), bla KPC (22/84; 26.2%), bla VIM (19/84; 22.6%), and bla IMP (12/84; 14.3%) families. The reported mobile genetic elements (MGE) carrying carbapenemase-encoding genes included plasmids (16/19; 84.2%), integrons (3/19; 15.8%), transposons (3/19; 15.8%), and insertion sequences (2/19; 10.5%). bla OXA-48 was often carried by (60kb-65kb) IncL/M-type pOXA-48 plasmids, while bla NDM-5 was often carried by (45–50kb) IncX-type plasmids. Moreover, 25 articles investigated and reported virulent and hypervirulent CRBs that carried multiple virulence factors. Conclusion Animal-environment-food ecosystems would constitute reservoirs of CRBs involved in human infections. The One Health approach and constant collaboration between governments are necessary to drastically reduce the mortality rates linked to antimicrobial resistance.

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Section: Resultsmentioning

confidence: 99%

Carbapenem Resistance in Animal-Environment-Food from Africa: A Systematic Review, Recommendations and Perspectives

Dossouvi,

Ametepe

2024

IDR

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“…The results were entered into the SPSS version 16 software (IBM Armonk, NY, USA) and statistical analysis was carried out using appropriate tests such as chi-square. In this test, P -value was considered statistically significant ≤ 0.05 (Ejikeugwu et al 2019 ; Sheikh et al 2020 ). For LAMP and M-PCR, the specificity, sensitivity, positive predictive value (PPV), and negative predictive value (NPV) were calculated and compared to the culture results.…”

Section: Methodsmentioning

confidence: 99%

Loop mediated isothermal amplification of Clostridioides difficile isolates in gastrointestinal patients

et al. 2022

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This study investigated the prevalence of Clostridioides difficile by culture, multiplex polymerase chain reaction (M-PCR), and loop mediated isothermal amplification (LAMP) in patients with suspected C. difficile infections (CDIs). Also, the results of three methods were compared. All stool specimens collected from CDI suspected patients were cultured on selective C. difficile cycloserine-cefoxitin fructose agar and incubated in an anaerobic jar up to 7 days. The bacterial isolates were identified using standard tests. Multiplex-PCR (M-PCR) was performed for detection of tcdA, tcdB, and tpi genes. The LAMP assay was performed to detect the tcdB gene of C. difficile. C. difficile was isolated from 20.0% (n = 10/50) of samples by culture. M-PCR showed that 34.0% (n = 17/50) of the specimens were positive for C. difficile based on the presence of tpi gene. Out of the 17 C. difficile, 13 strains (76.0%) were positive for tcdB gene using M-PCR. However, the LAMP assay showed that 30.0% (15/50) of specimens were positive for the presence of tcdB gene. M-PCR and LAMP methods showed 100.0% sensitivity compared to the culture method. However, the specificity of the LAMP (87.5%) was relatively higher than the M-PCR (82.5%) compared to the culture. Based on the results of this study, the prevalence of toxigenic C. difficile strains was high in suspected CDI patients. So, the differentiation between toxigenic and non-toxigenic strains is necessary. Our data showed that the LAMP assay is a good method for direct detection of toxigenic C. difficile strains from stool specimens.

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“…A total of 10 microbial isolates comprising of Gram-positive bacteria ( Staphylococcus aureus , Bacillus subtilis ), Gram-negative bacteria ( Escherichia coli , Pseudomonas aeruginosa , Klebsiella pneumoniae ), yeast ( Candida albicans ) and dermatophytic pathogens ( Trichophyton schoenleinii, Trichophyton mentagrophytes, Microsporum gypseum, and Microsporum ferugineum ), previously collected from clinical samples in the postgraduate laboratory of the Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, were used in this study. Each of the collected isolates was re-identified and confirmed using the standard protocols as described in the literature [ [19] , [20] , [21] , [22] , [23] , [24] , [25] ].…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the phytoconstituents of Auricularia auricula-judae mushroom and antimicrobial activity of its protein extract

Oli

Edeh

Al-Mosawi

et al. 2020

European Journal of Integrative Medicine

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Introduction The growing resistance to antibiotics and the complexity of defeating multi-drug resistant bacteria have led to an increase in the search for novel and effective antimicrobials from various plants. This study aimed to determine the bioactive contents of Auricularia auricular-judae mushroom and evaluate the antimicrobial potential of its protein extract against some selected human bacterial and fungal pathogens which could serve as a lead to the discovery of new antimicrobial agents. Methods The constituents of the A. auricular-judae were evaluated by standard phytochemical analysis methods. The agar well diffusion, micro-broth dilution, and time-kill kinetic assays were used to determine the antimicrobial activity of the extracts against Staphylococcus aureus , Bacillus subtilis , Escherichia coli , Pseudomonas aeruginosa , Klebsiella pneumoniae , yeast ( Candida albicans ), and dermatophytic pathogens. Results The preliminary phytochemical analysis of the extracts revealed the presence of carbohydrate (43.15 %; 38.30 %) proteins (23.75 %; 23.75 %), flavonoids (1.20 %; 0.80 %), alkaloids (0.60 %; 1.00 %), saponin (6.00 %; 2.40 %), tannin (1.65 %; 1.57 %), cyanide (0.24 %; 0.40 %), ash (12.40 %; 10.40 %), moisture (6.00 %;6.00 %), lipids(6.00 %;6.00 %), and fiber (8.70 %; 6.45 %) for the Tris buffer and warm aqueous extracts, respectively. The Tris and warm aqueous protein extracts showed antimicrobial effects toward all the human bacterial pathogens and two fungal isolates. Conclusions This study revealed the potential ability of A. auricula-judae for use as a herbal antimicrobial in the treatment of human bacterial and fungal pathogens.

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Characterization of metallo-β-lactamases-encoding genes blaIMP-1 and blaVIM-1 amongst Klebsiella pneumoniae from abattoir samples of Ebonyi state, southeastern Nigeria

Cited by 7 publications

References 9 publications

Carbapenem Resistance in Animal-Environment-Food from Africa: A Systematic Review, Recommendations and Perspectives

Carbapenem Resistance in Animal-Environment-Food from Africa: A Systematic Review, Recommendations and Perspectives

Loop mediated isothermal amplification of Clostridioides difficile isolates in gastrointestinal patients

Evaluation of the phytoconstituents of Auricularia auricula-judae mushroom and antimicrobial activity of its protein extract

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