Characterization of methane, benzene and toluene-oxidizing consortia enriched from landfill and riparian wetland soils

Lee, Eun Hee; Park, Hyun-Jung; Cho, Kyung-Suk

doi:10.1016/j.jhazmat.2010.08.038

Cited by 33 publications

(15 citation statements)

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“…The two bacteria originated from soil, but were not isolated from the same consortium. The obligate methanotroph M6 [15] was maintained in nitrate mineral salts (NMS) medium with 50,000 ppm methane as previously described by [16]. NMS medium contained MgSO 4 ∙7H 2 O 1 g L −1 , CaCl 2 ∙2H 2 O 0.134 g L −1 , KNO 3 1 g L −1 , KH 2 PO 4 0.272 g L −1 , Na 2 HPO 4 ∙12H 2 O 0.717 g L −1 [29].…”

Section: Methodsmentioning

confidence: 99%

Density-dependent enhancement of methane oxidation activity and growth of Methylocystis sp. by a non-methanotrophic bacterium Sphingopyxis sp

Jeong

Cho

Kim

2014

Biotechnology Reports

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Methanotrophs are a biological resource as they degrade the greenhouse gas methane and various organic contaminants. Several non-methanotrophic bacteria have shown potential to stimulate growth of methanotrophs when co-cultured, and however, the ecology is largely unknown. Effects of Sphingopyxis sp. NM1 on methanotrophic activity and growth of Methylocystis sp. M6 were investigated in this study. M6 and NM1 were mixed at mixing ratios of 9:1, 1:1, and 1:9 (v/v), using cell suspensions of 7.5 × 1011 cells L−1. Methane oxidation of M6 was monitored, and M6 population was estimated using fluorescence in situ hybridization (FISH). Real-time PCR was applied to quantify rRNA and expression of transcripts for three enzymes involved in the methane oxidation pathway. NM1 had a positive effect on M6 growth at a 1:9 ratio (p < 0.05), while no significant effects were observed at 9:1 and 1:1 ratios. NM1 enhanced the methane oxidation 1.34-fold at the 1:9 ratio. NM1 increased the population density and relative rRNA level of M6 by 2.4-fold and 5.4-fold at the 1:9 ratio, indicating that NM1 stimulated the population growth of M6. NM1 increased the relative transcriptional expression of all mRNA targets only at the 1:9 ratio. These results demonstrated that NM1 enhanced the methanotrophic activity and growth of M6, which was dependent on the proportion of NM1 present in the culture. This stimulation can be used as management and enhancement strategies for methanotrophic biotechnological processes.

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Section: Methodsmentioning

confidence: 99%

Density-dependent enhancement of methane oxidation activity and growth of Methylocystis sp. by a non-methanotrophic bacterium Sphingopyxis sp

Jeong

Cho

Kim

2014

Biotechnology Reports

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“…Bacteria and fungi were shown to oxidize methane, benzene, and toluene in soil and snowpack as a result of metabolic activity (Lee et al, 2010). Bio-organic materials (e.g., algae bloom) are known to affect the snow color (Bej et al, 2010) and therefore reflectivity (albedo) of snow surfaces.…”

Section: Biological Sources/sinksmentioning

confidence: 99%

Organics in environmental ices: sources, chemistry, and impacts

et al. 2012

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Abstract. The physical, chemical, and biological processes involving organics in ice in the environment impact a number of atmospheric and biogeochemical cycles. Organic material in snow or ice may be biological in origin, deposited from aerosols or atmospheric gases, or formed chemically in situ. In this manuscript, we review the current state of knowledge regarding the sources, properties, and chemistry of organic materials in environmental ices. Several outstanding questions remain to be resolved and fundamental data gathered before an accurate model of transformations and transport of organic species in the cryosphere will be possible. For example, more information is needed regarding the quantitative impacts of chemical and biological processes, ice morphology, and snow formation on the fate of organic material in cold regions. Interdisciplinary work at the interfaces of chemistry, physics and biology is needed in order to fully characterize the nature and evolution of organics in the cryosphere and predict the effects of climate change on the Earth's carbon cycle.

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“…Methane (CH 4 ) is considered to be the second major greenhouse gas (GHG) contributing to 18% of the total atmospheric radiative forcing (Dunfield, 1580 O. P. Karthikeyan et al 2007; Lee et al, 2010). The global warming potential of CH 4 relative to CO 2 was calculated as 25 or 72 times higher over a 100 or 20 year period, respectively (Torres-Alvarado et al, 2005;Nikiema et al, 2007;Kabir and Halim, 2011).…”

Section: Introductionmentioning

confidence: 99%

Review of Sustainable Methane Mitigation and Biopolymer Production

Karthikeyan

Chidambarampadmavathy

Cirés

et al. 2014

Critical Reviews in Environmental Science and Technology

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Biological methane (CH 4 ) mitigation combined with biopolymer (PHA-polyhydroxyalkaonate/PHB-polyhydroxybutyrate) production is a viable option in this fossil fuel-constrained era.Methantrophs are bacteria that can re-route CH 4 into PHA/PHB under nutrient-starved conditions. However, most studies (up to 90%) investigated pure cultures to demonstrate capacity for PHA/PHB accumulation, which on an industrial scale is unlikely to be serviceable. Furthermore, commercialization is handicapped as there are still a number of unresolved issues which affect productivities, such as optimized process variables, characterization of robust consortia, and optimized reactor design. This review will summarize existing knowledge and highlight research needs to fast track methanotrophic PHA/PHB production from CH 4 .

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Characterization of methane, benzene and toluene-oxidizing consortia enriched from landfill and riparian wetland soils

Cited by 33 publications

References 33 publications

Density-dependent enhancement of methane oxidation activity and growth of Methylocystis sp. by a non-methanotrophic bacterium Sphingopyxis sp

Density-dependent enhancement of methane oxidation activity and growth of Methylocystis sp. by a non-methanotrophic bacterium Sphingopyxis sp

Organics in environmental ices: sources, chemistry, and impacts

Review of Sustainable Methane Mitigation and Biopolymer Production

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