Characterization of methicillin-resistant and enterotoxins producing Staphylococcus aureus in bovine milk in India

Mahanti, Achintya; Joardar, Siddhartha Narayan; Bandyopadhyay, Samiran; Banerjee, Jaydeep; Ghosh, S. K.; Batabyal, K.; Sar, Tapas Kumar; Dutta, Tapan Kumar; Samanta, Indranil

doi:10.1016/j.jafr.2019.100017

Cited by 15 publications

(17 citation statements)

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“…In a third study, where 16 isolates were identified as MRSA, 50% of the isoltes belonged to ST1687 31 . Another recent report from West Bengal described the existence of t304 and t6267 types among nine MRSA isolates 32 . In this study, we analysed 166 isolates of S. aureus obtained from bovine mastitis cases from six different states of India using spa , MLST and PFGE, and found the existence of limited CCs with diversity at genotype level.…”

Section: Introductionmentioning

confidence: 98%

Molecular fingerprinting of bovine mastitis-associated Staphylococcus aureus isolates from India

Annamanedi

Sheela

Sundareshan

et al. 2021

Sci Rep

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Staphylococcus aureus is a major etiological agent of clinical and subclinical bovine mastitis. Owing to the mostly backyard dairy practices, we hypothesized that genetic diversity among mastitis-associated S. aureus from India would be high, and investigated 166 isolates obtained mostly from the Southern State of Karnataka, but also from a few other states. The results revealed (a) 8 to 13 fragments in pulsed-field gel electrophoresis (PFGE), forming 31 distinct patterns, and (b) 34 spa types, of which three (t17680, t18314, and t18320) were newly identified. Multi-locus sequencing typing (MLST) identified 39 sequence types (STs), with ST2454 (34.4%) and ST2459 (24%) being the most commonly represented, which clustered to clonal complexes (CC) CC9 and CC97, respectively; 12 STs were newly identified. Thirty-four (20.5%) of the 166 isolates displayed oxacillin resistance. On the other hand, whereas none were mecC+, 44 (26.5%) isolates were mecA+, with a predominance of SCCmecIVb (26/32 isolates, others being untypeable); 24 isolates (14.46%) were oxacillin-susceptible methicillin-resistant S. aureus (OS-MRSA; mecA+ but OS). Integrated analysis revealed that CC9-ST2454- and CC97-ST2459-SCCmecIVb were the predominant MRSA, although the distribution of CC9 and CC97 was similar between methicillin-resistant and -susceptible isolates. By PCR, 56.25%, 28.75% and 47.5% of the 166 isolates were positive for hlg, tsst and pvl genes, respectively. Our results, for the first time describe the application of a combination of various molecular methods to bovine mastitis-associated S. aureus isolates from India, corroborate the worldwide distribution of CC97 and CC9, and suggest pathogenic potential of the isolates.

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Section: Introductionmentioning

confidence: 98%

Molecular fingerprinting of bovine mastitis-associated Staphylococcus aureus isolates from India

Annamanedi

Sheela

Sundareshan

et al. 2021

Sci Rep

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“…Sizes of squares indicate the weight of each study based on sample size. The diamond indicates the estimated pooled effect size; CI = confidence interval Overall (I^2 = 97•48%, p = 0•00) Buyukcangaz et al, 2013 Zayda et al, 2020 Kwon et al, 2005 Zaatout et al, 2020 Awad et al, 2017 Guimarães et al, 2017 Elhaig and Selim, 2015 El-Ashker et al, 2015 Akkou et al, 2018 Algammal et al, 2020 Tavakol et al, 2012 Oreiby et al, 2019 Chandrasekaran et al, 2014 Rossi et al, 2019 Pu et al, 2014 Yang et al, 2020 Dan et al, 2019 Sayed, 2016 Klibi et al, 2018 Study Havaei et al, 2015 Enany et al, 2013 Elkenany, 2018 Nazir et al, 2017 Hamid et al, 2017 Girmay et al, 2020 Kutar et al, 2015 Jamali et al, 2014 Wang et al, 2018 Yi et al, 2018 Pehlivanoglu and Yardımcı, 2012 El-Ashker et al, 2020 Zhang et al, 2016Gindonis et al, 2013Käppeli et al, 2019Schabauer et al, 2018Benhamed and Kihal, 2013Mistry et al, 2016Abed et al, 2018Zuniga et al, 2020Shah et al, 2019Khan et al, 2020 Haveri et al, 2008Song et al, 2016Silva et al, 2014Heidari et al, 2011Ismail, 2017 Shrivastava et al, 2016Ganai et al, 2016Srednik et al, 2018Hanon, 2016Mahanti et al, 2020Kaszanyitzky et al, 2007Abd El-Tawab et al, 2016Suleiman et al, 2012Asfour, 2011 Abd Al-Azeem et al, 2013Sheela et al, 2016Hoque et al, 2018Srednik et al, 2019Kumar et al, 2011Kumar et al, 2010 Schmidt et al, 2015Moon et al, 2007Kumari et al, 2020Patel et al, 2017Salam, 2015Li et al, 2015…”

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confidence: 99%

A meta‐analysis of the global prevalence of methicillin‐resistantStaphylococcus aureus(MRSA) isolated from clinical and subclinical bovine mastitis

Zaatout

Hezil

2021

J of Applied Microbiology

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Aims: This meta-analysis aims to assess the point prevalence of methicillin-resistant Staphylococcus aureus (MRSA) isolated from bovine mastitis cases at the global level. Methods and Results: Several electronic databases were searched for relevant publications (PubMed, EMBASE, Web of Knowledge and Cochrane Library). Heterogeneity between studies was assessed using the Cochrane Q test and I 2 test statistics based on the random-effect model. The potential sources of between-study heterogeneity were evaluated using subgroup analysis and meta-regression. Sensitivity and publication bias analyses were performed. Sixty-six studies with a total of 77,644 mastitis cases were eligible and included in the analysis. The overall pooled prevalence of MRSA was 4•30% (95% CI: 3•24-5•50) with a significant heterogeneity (I 2 = 97•48%, p < 0•001). In the subgroup analysis by region, the highest prevalence was found in Asia (6•47%, 95% CI: 4•33-8•97), and the lowest prevalence was reported in Europe (1•18%, 95% CI: 0•18-2•83).The pooled prevalence was significantly higher in clinical mastitis and cases published during 2016-2020. Conclusions:The results of this study suggest that there is a lower prevalence of MRSA in bovine mastitis. However, its prevalence increased in the past 4 years. Therefore, continuous surveillance is urgently required for monitoring the dissemination of these clinically important bacteria. Significance of the Study:To our knowledge, this is the first meta-analysis to estimate the global prevalence of MRSA isolated from bovine mastitis cases.

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“…In another study in West Bengal, a total of 450 composite milk samples both from cattle with or without mastitis were collected. Nine (9.6%) S. aureus isolates were detected as MRSA and all the MRSA isolates were multidrug resistant (Mahanti et al 2020).…”

Section: Evm and Antimicrobial Resistance (Amr)mentioning

confidence: 99%

Ethnoveterinary medicine for responsible dairying

Rath

Sharma

Joshi

2020

IJDS

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Characterization of methicillin-resistant and enterotoxins producing Staphylococcus aureus in bovine milk in India

Cited by 15 publications

References 45 publications

Molecular fingerprinting of bovine mastitis-associated Staphylococcus aureus isolates from India

Molecular fingerprinting of bovine mastitis-associated Staphylococcus aureus isolates from India

A meta‐analysis of the global prevalence of methicillin‐resistantStaphylococcus aureus(MRSA) isolated from clinical and subclinical bovine mastitis

Ethnoveterinary medicine for responsible dairying

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