Characterization of methyl parathion degradation by a Burkholderia zhejiangensis strain, CEIB S4-3, isolated from agricultural soils

Abstract

Through the use of an enrichment technique, we isolated from the agricultural soils of Morelos in central México a strain of Burkholderia zhejiangensis identified as CEIB S4-3, it's could use the pesticide methyl parathion (MP) as the only source of carbon and degrade completely p-nitrophenol (PNP). For more efficient MP and PNP degradation by the CEIB S4-3 strain, the absence of an extra carbon source, a large inoculum and an MP concentration up to 50 mg/l are required. Sequence and annotation analysis of the draft genome, showed presence of mpd functional gene, which was expressed and its activity on the MP was confirmed. Additionally, the genes coding for enzymes in the benzoquinone pathway (conducted by Gram-negative bacteria) and the benzenotriol pathway (conducted by Gram-positive bacteria) were found, which was corroborated by identification of intermediary metabolites by HPLC. Thus, we propose that B. zhejiangensis CEIB S4-3 uses both degradation pathways.

Transcriptional analysis reveals the metabolic state ofBurkholderia zhejiangensisCEIB S4-3 during methyl parathion degradation

“…The genomic data revealed that B. zhejiangensis CEIB S4-3 has a methyl parathion degrading ( mpd ) gene with 99% identity with respect to the gene MpdB, which was identified in B. cepacia by Ekkhunnatham et al (2012), which codes for a methyl parathion hydrolase (MPH) enzyme. Furthermore, this data also reveals that this strain has two PNP catabolic gene clusters, pnpABA′E1E2FDC and pnpE1E2FDC (Hernández-Mendoza et al, 2014; Popoca-Ursino et al, 2017). The alignment between the two PNP proteins of B. zhejiangensis CEIB S4-3 and Burkholderia sp.…”

“…Previously, information about the strain Burkholderia zhejiangensis CEIB S4-3, which was isolated from agricultural soils from the state of Morelos, in central Mexico, was reported. This strain was found to have the ability to hydrolyze MP, to degrade PNP and to use the byproducts as a carbon source when cultured in the presence of 0.2 mM of MP in liquid fermentation (Popoca-Ursino et al, 2017). The strain B. zhejiangensis CEIB S4-3 is able to hydrolyze MP pesticide in 1 h and completely degrade PNP in 12 h. The analysis of a draft genome of this strain (Hernández-Mendoza et al, 2014) revealed the presence of several genes that encode sequences that are highly similar to previously characterized enzymes related to the hydrolysis of MP and the degradation of PNP in Burkholderia sp.…”

“…The alignment between the two PNP proteins of B. zhejiangensis CEIB S4-3 and Burkholderia sp. SJ98 showed an identity of 67% and 100%, respectively; a similarity of 79% and 100%, respectively; and an E -value of 3 e −71 and 0.0, respectively (Popoca-Ursino et al, 2017; Vikram et al, 2012, 2013).…”

Biodegradation of Methyl Parathion Using Pseudomonas stutzeri (MTCC 2643)

“…In the present study, the retention time for standard methyl parathion was 3.433 minutes with the peak area of 7.345 mV.s and peak height of 0.444 mV (Fig.8) in HPLC analysis. Burkholderia cepacia which has a potential methyl parathion degrading ability from agricultural soil was isolated [44]. Table 1…”

Soil Microorganisms and Their Potential in Pesticide Biodegradation; A Review Article

“…Mangrove peat (Natália Alvarenga et al, 2018) Burkholderia sp. (Fernández-López et al, 2017;Popoca-Ursino, Martinez-Ocampo, Dantan-Gonzalez, Sanchez-Salinas and Ortiz-Hernandez, 2017) Klebsiella sp. (Pattanayak, Chakraborty, Biswas, Chattopadhyay and Chakraborty, 2018) Fischerella sp.…”