2017
DOI: 10.1002/cbic.201700216
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Characterization of Methyltransferase AlmCII in Chalcomycin Biosynthesis: The First TylF Family O‐Methyltransferase Works on a 4′‐Deoxysugar

Abstract: Sugar O-methylation is a ubiquitous modification in natural products and plays diverse roles. This realization has inspired many attempts to search for novel methyltransferases. Chalcomycins are a group of 16-membered macrolides containing two methylated sugars that require three methyltransferases for their biosynthesis. Here, we identified that AlmCII, a sugar O-methyltransferase belonging to the TylF family that was previously only known to methylate sugars with a 4'-hydroxy group, can methylate a 4',6'-did… Show more

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Cited by 6 publications
(4 citation statements)
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“…Antibacterial activity of compounds 1 , 3 , 4 , and 5 against Gram-positive strain Staphylococcus aureus 209 P was evaluated using the 2-fold dilution assay17., 18., 19.. Seed cultures of S. aureus 209 P was inoculated on beef extract medium at 37 °C for 1 day.…”
Section: Methodsmentioning
confidence: 99%
“…Antibacterial activity of compounds 1 , 3 , 4 , and 5 against Gram-positive strain Staphylococcus aureus 209 P was evaluated using the 2-fold dilution assay17., 18., 19.. Seed cultures of S. aureus 209 P was inoculated on beef extract medium at 37 °C for 1 day.…”
Section: Methodsmentioning
confidence: 99%
“…The regiospecific chemical synthesis of O -methylated sugars is challenging and unsustainable because it requires (i) harsh catalytic conditions, (ii) the release of toxic by-products, (iii) a long reaction time, (iv) protection/deprotection of unwanted groups, and (v) expensive reagents. Nevertheless, biocatalytic methylation using enzymes and engineered microorganisms are capable of O -methylation of compounds for producing novel bioactive compounds in a much milder and single-step reaction using resources from microbial cells ( 26 , 27 ).…”
Section: Introductionmentioning
confidence: 99%
“…These compounds were tested for antimicrobial activity against Gram-positive Staphylococcus aureus 209P by the 2-fold dilution method. 22 Most of the compounds showed potent inhibitory activity (Table S3 in the Supporting Information). Among them, cephalosporin P 1 is the most active compound with a MIC value of 0.06 μg mL −1 .…”
mentioning
confidence: 99%
“…By heterologous expression in A. oryzae , we obtained cephalosporin P 1 and 10 other analogues, including five new compounds. These compounds were tested for antimicrobial activity against Gram-positive Staphylococcus aureus 209P by the 2-fold dilution method . Most of the compounds showed potent inhibitory activity (Table S3 in the Supporting Information).…”
mentioning
confidence: 99%