Characterization of microbial community and kinetics for spent sulfidic caustic applied autotrophic denitrification

Byun, Imgyu; Park, JeungJin; Park, SoRa; Lee, Tae‐Ho; Park, Taejoo

doi:10.1007/s12257-007-0181-y

Cited by 12 publications

(9 citation statements)

References 19 publications

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“…It suggests that other nitrite-oxidizing bacteria, such as Nitrospira, played an important role for nitrification. In the future study, another probe specified for Nitrospira will be used for a detection of nitrite oxidizing bacteria [16,17].…”

Section: Presence Of Káíêçëçãçå~ë and Káíêçä~åíéêmentioning

confidence: 99%

Microbial community structures in conventional activated sludge system and membrane bioreactor (MBR)

Baek

Pagilla

2009

Biotechnol Bioproc E

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The microbial community structures of a conventional activated sludge and MBR systems treating the municipal wastewater were studied using Fluorescent áåJëáíì Hybridization (FISH) analysis to identify differences in both systems. The oligonucleotide probes specific for overall bacteria, including α-, β-, and γ-subclasses of mêçíÉçÄ~ÅíÉêá~, ammonia-oxidizing bacteria (káíêçëçãçå~ë), and nitrite-oxidizing bacteria (káíêçÄ~ÅíÉê) were used to compare the microbial community structure of both systems. A trend of less hybridization with bacteria-specific probe EUB338 was observed in MBR systems operated under aerobic condition, compared to conventional activated sludge system. The less hybridization trend with the probes could be associated with low ribosomal RNA (rRNA) content in the biomass, which suggests that the biomass in the MBR system was not in a physiological state characteristic for growth due to low substrate per unit biomass. © KSBB hÉóïçêÇëW=ÅçåîÉåíáçå~ä=~Åíáî~íÉÇ=ëäìÇÖÉI=çäáÖçåìÅäÉçíáÇÉ=éêçÄÉI=ãÉãÄê~åÉ=ÄáçêÉ~ÅíçêI=ÑäìçêÉëÅÉåí=áåJëáíì=ÜóÄêáÇáò~íáçå= = = = =

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Section: Presence Of Káíêçëçãçå~ë and Káíêçä~åíéêmentioning

confidence: 99%

Microbial community structures in conventional activated sludge system and membrane bioreactor (MBR)

Baek

Pagilla

2009

Biotechnol Bioproc E

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“…Compared to the other sequence-based diagnostic methods, such as RFLP, DGGE, and denaturing HPLC, CE-SSCP offers the advantages of simplicity, speed, sensitivity, and versatility [15][16][17]. However, the conventional polymer matrix has limitations because of its relatively hydrophobic nature [6,7].…”

Section: Discussionmentioning

confidence: 99%

A novel pathogen detection system based on high‐resolution CE‐SSCP using a triblock copolymer matrix

Shin

Hwang

Seo

et al. 2010

J of Separation Science

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Although CE-SSCP analysis combined with 16S ribosomal RNA gene-specific PCR has enormous potential as a simple and versatile pathogen detection technique, low resolution of CE-SSCP causes the limited application. Among the experimental conditions affecting the resolution, the polymer matrix is considered to be most critical to improve the resolution of CE-SSCP analysis. However, due to the peak broadening caused by the interaction between hydrophobic moiety of polymer matrices and DNA, conventional polymer matrices are not ideal for CE-SSCP analysis. A poly(ethyleneoxide)-poly(propyleneoxide)-poly(ethyleneoxide) (PEO-PPO-PEO) triblock copolymer, with dynamic coating ability and a propensity to form micelles to minimize exposure of hydrophobic PPO block to DNA, can be an alternative matrix. In this study, we examined the resolution of CE-SSCP analysis using the PEO-PPO-PEO triblock copolymer as the polymer matrix and four same-sized DNA fragments of similar sequence content. Among 48 commercially available PEO-PPO-PEO triblock copolymers, three were selected due to their transparency in the operable range of viscosity and PEO(137)PPO(43)PEO(137) exhibited the most effective separation. Significant improvement in resolution allowed discrimination of the similar sequences, thus greatly facilitated CE-SSCP analysis compared to the conventional polymer matrix. The results indicate that PEO-PPO-PEO triblock copolymer may serve as an ideal matrix for high-resolution CE-SSCP analysis.

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“…Spent sulfidic caustic (SSC) could also be a potential source of both sulfide and alkalinity for autotrophic denitrification. SSC is a waste product from petroleum refining process and has been studied in few denitrification applications Byun et al, 2008;Park et al, 2008). Neither of these sulfide-generating methods were investigated for this study; however they are discussed in some detail later.…”

Section: Literature Reviewmentioning

confidence: 99%

Evaluation of Sulfide-driven Autotrophic Denitrification in a Moving Bed Biofilm Reactor

Bill¹,

Bott²,

Murthy³

2010

proc water environ fed

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While the application of alternative substrates in suspended growth processes has been studied extensively, fixed film post denitrification processes have been designed to use primarily low yield substrates like methanol. Autotrophic denitrification using reduced sulfur compounds could be a low yield alternative to conventional heterotrophic denitrification to polish effluent nitrate. Three lab-scale MBBRs (using sulfide, methanol and ethanol as electron donor sources) were operated continuously at 12°C. The performance of each reactor was monitored by weekly sampling and batch rate profile testing. Sulfide visually exhibited biofilm carrier biomass content similar to or slightly less than that of methanol, yet provided increased removal rates (1.1±0.5 versus 0.6±0.3 g NO X -N/m 2 /day, respectively.) SDNR profile testing also suggested that maximum NO X -N removal rates for sulfide (1.8 g NO X -N/m 2 /day) were more than three times that of methanol (0.57 g NO X -N/m 2 /day.) Although the sulfide reactor produced the highest removal rates, significant uncertainties must be addressed prior to full-scale implementation.

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Characterization of microbial community and kinetics for spent sulfidic caustic applied autotrophic denitrification

Cited by 12 publications

References 19 publications

Microbial community structures in conventional activated sludge system and membrane bioreactor (MBR)

Microbial community structures in conventional activated sludge system and membrane bioreactor (MBR)

A novel pathogen detection system based on high‐resolution CE‐SSCP using a triblock copolymer matrix

Evaluation of Sulfide-driven Autotrophic Denitrification in a Moving Bed Biofilm Reactor

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