Characterization of Microbial Community in Cold-Chain Hairtail Fish by High-Throughput Sequencing Technology

Abstract

High-throughput sequencing was used to analyze the microbial communities in the muscle samples of hairtail fish to study their diversity and dynamic changes during cold-chain circulation. The results showed that the richness and diversity of the microbial community in hairtail fish had a transient decline in 0–24 h and decreased after the first rise during 24–216 h. The diversity and richness of bacteria in cold-chain hairtail fish reached the maximum at 168 h. The Shannon and Simpson diversity indices of the bacteria were 2.96 and 0.16, respectively, and their ACE and Chao1 richness indices were 254.84 and 155.10, respectively. In addition, the dominant bacteria were Proteobacteria in the phylum level, Gammaproteobacteria in the class level, Pseudomonadales in the order level, Pseudomonadaceae in the family level, and Pseudomonas in the genus level, and their relative abundance were 80.52%, 72.11%, 76.68%, 23.25%, and 53.50%, respectively. In this study, the structure of bacterial flora and the dominant bacteria in cold-chain hairtail fish were analyzed by high-throughput sequencing to provide a basis for exploring how to maintain the freshness of hairtail fish and for predicting the shelf-life of hairtail fish.

Changes in the Physicochemical Properties and Microbial Communities of Air-Fried Hairtail Fillets during Storage

“…The hairtail ( Trichiurus lepturus ), a highly prized marine fish species, is widely distributed across the eastern Pacific Ocean and along the coastlines of various regions in China [ 1 ]. Among China’s four major commercial fish species, the hairtail is notably rich in vitamins, minerals, polyunsaturated fatty acids, proteins, and essential amino acids, such as eicosatetraenoic acid and docosahexaenoic acid [ 2 , 3 ]. Consequently, several hairtail products, including air-dried hairtail [ 4 ], vinasse hairtail fillets [ 5 ], and deep-fried hairtail fillets [ 6 ], have been developed.…”

Quality Grading and Prediction of Frozen Zhoushan Hairtails in China Based on ETSFormer

“…In order to improve the above problem, many related scholars are committed to studying the quality change in hairtail during logistics and transportation and its maintenance methods. Scholars analyzed microbial communities in hairtail muscle samples using the Illumina MiSeq high-throughput DNA sequencing platform [15]. This method can be used to study the diversity and dynamics of microbial communities in hairtail during the cold chain.…”

Microbiome data reveal significant differences in the bacterial diversity in freshwater rohu (Labeo rohita) across the supply chain in Dhaka, Bangladesh

“…It is therefore important to characterize the microbiota of this fish in different stages of post-harvest to identify the sources of contamination. Owing to the limitations of plate-based morphological and biochemical characterization, culture-independent high-throughput sequencing (HTS) has been used to trace down the potential sources of microbial contamination in post-harvest fishes and food products (Bledsoe et al 2016;Antunes-Rohling et al 2019;Xing et al 2021). In this study, we aimed to identify and compare the skin and gut microbiota of live, frozen and marketed rohu fish samples, with the aid of culture-independent quantitative real-time polymerase chain reaction (RT-PCR) and 16S rRNA gene sequencing technology.…”

“…2019; Xing et al . 2021). In this study, we aimed to identify and compare the skin and gut microbiota of live, frozen and marketed rohu fish samples, with the aid of culture‐independent quantitative real‐time polymerase chain reaction (RT‐PCR) and 16S rRNA gene sequencing technology.…”