Characterization of Microbial Populations of Lake Van by 16s Metagenomics Study

Poyraz, Nilgün; Mutlu, Mehmet

doi:10.18036/estubtdc.680795

Cited by 3 publications

(1 citation statement)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Microbial populations present in water of Lake Van have been previously examined and a NGS study was performed with a water sample taken from only one point. As a result of this study, it was determined that Proteobacteria, Actinobacteria, and Verrucomicrobia were dominant at phylum-level 50 . However, there is a known fact that sampling is essential in microbiology and sampling must be done to represent the entire habitat to determine the actual microbial load and diversity.…”

Section: Discussionmentioning

confidence: 90%

Microbial community of soda Lake Van as obtained from direct and enriched water, sediment and fish samples

Omeroglu

Sudağıdan

Yurt

et al. 2021

Sci Rep

View full text Add to dashboard Cite

Soda lakes are saline and alkaline ecosystems that are considered to have existed since the first geological records of the world. These lakes support the growth of ecologically and economically important microorganisms due to their unique geochemistry. Microbiota members of lakes are valuable models to study the link between community structure and abiotic parameters such as pH and salinity. Lake Van is the largest endroheic lake and in this study, bacterial diversity of lake water, sediment, and pearl mullet (inci kefali; Alburnus tarichi), an endemic species of fish which are collected from different points of the lake, are studied directly and investigated meticulously using a metabarcoding approach after pre-enrichment. Bacterial community structures were identified using Next Generation Sequencing of the 16S rRNA gene. The analysis revealed that the samples of Lake Van contain high level of bacterial diversity. Direct water samples were dominated by Proteobacteria, Cyanobacteria, and Bacteroidota, on the other hand, pre-enriched water samples were dominated by Proteobacteria and Firmicutes at phylum-level. In direct sediment samples Proteobacteria, whereas in pre-enriched sediment samples Firmicutes and Proteobacteria were determined at highest level. Pre-enriched fish samples were dominated by Proteobacteria and Firmicutes at phylum-level. In this study, microbiota members of Lake Van were identified by taxonomic analysis.

show abstract

Section: Discussionmentioning

confidence: 90%

Microbial community of soda Lake Van as obtained from direct and enriched water, sediment and fish samples

Omeroglu

Sudağıdan

Yurt

et al. 2021

Sci Rep

View full text Add to dashboard Cite

show abstract

Spatiotemporal structure and composition of the microbial communities in hypersaline Lake Magadi, Kenya

Kipnyargis,

Kenya,

Khamis

et al. 2024

F1000Res

View full text Add to dashboard Cite

Background Soda lakes are habitats characterized by haloalkaline conditions also known to host unique microbial communities. The water chemistry changes with seasons due to evaporative concentration or floods from the surrounding grounds. However, it is not yet clear if the change in physiochemical changes influences the spatiotemporal diversity and structure of microbial communities in these ecosystems. Methods This study investigated the spatiotemporal diversity and structure of microbial communities in water and brine samples collected from hypersaline Lake Magadi in the months of June–September 2018. Amplicons were generated using Illumina sequencing of the 16S rRNA gene. Results The abundant bacterial phyla were Proteobacteria, Cyanobacteria, Bacteroidetes, Actinobacteria, Firmicutes, Verrumicrobia, Deinococcus-Thermus, Spirochaetes, and Chloroflexi. The Archaeal diversity was represented by phyla Euryachaeota, Crenarchaeota, Euryarchaeota, and Thaumarchaeota. The dominant bacterial species were: Euhalothece sp. (10.3%), Rhodobaca sp. (9.6%), Idiomarina sp. (5.8%), Rhodothermus sp. (3.0%), Roseinatronobacter sp. (2.4%), Nocardioides sp. (2.3%), Gracilimonas sp. (2.2%), and Halomonas sp. (2%). The dominant archaeal species included Halorubrum sp. (18.3%), Salinarchaeum sp. (5.3%), and Haloterrigena sp. (1.3%). The composition of bacteria was higher than that of archaea, while their richness and diversity varied widely across the sampling seasons. The α-diversity indices showed that high diversity was recorded in the month of August, followed by September, June, and July in that order. Furthermore, salinity and alkalinity affect β-diversity rather than the sampling site or seasonality. The effects of physicochemical parameters on the microbial community structure showed that temperature, pH, P+, K+, NO3 -, and total dissolved solids (TDS) had a positive correlation with the microbial community structure. Multivariate analysis revealed significant spatial and temporal effects on β-diversity and salinity and alkalinity were the major drivers of microbial composition in Lake Magadi. Conclusions We provide insights into the relationships between microbial communities and geochemistry across various sampling sites in Lake Magadi.

show abstract

Spatiotemporal structure and composition of the microbial communities in hypersaline Lake Magadi, Kenya

Kipnyargis,

Kenya,

Khamis

et al. 2024

F1000Res

View full text Add to dashboard Cite

Background Soda lakes are habitats characterized by haloalkaline conditions also known to host unique microbial communities. The water chemistry changes with seasons due to evaporative concentration or floods from the surrounding grounds. However, it is not yet clear if the change in physiochemical changes influences the spatiotemporal diversity and structure of microbial communities in these ecosystems. Methods Using 16S rRNA gene amplicon sequencing, we investigated the diversity and structure of microbial communities in water and brine samples taken from Lake Magadi between June and September 2018. Additionally, physicochemical parameters were also analyzed for every sampling site. Additionally, physicochemical parameters were also analyzed for every sampling site. Results The abundant bacterial phyla were Proteobacteria, Cyanobacteria, Bacteroidetes, Actinobacteria, Firmicutes, Verrumicrobia, Deinococcus-Thermus, Spirochaetes, and Chloroflexi. The Archaeal diversity was represented by phyla Euryachaeota, Crenarchaeota, Euryarchaeota, and Thaumarchaeota. The dominant bacterial species were: Euhalothece sp. (10.3%), Rhodobaca sp. (9.6%), Idiomarina sp. (5.8%), Rhodothermus sp. (3.0%), Roseinatronobacter sp. (2.4%), Nocardioides sp. (2.3%), Gracilimonas sp. (2.2%), and Halomonas sp. (2%). The dominant archaeal species included Halorubrum sp. (18.3%), Salinarchaeum sp. (5.3%), and Haloterrigena sp. (1.3%). The composition of bacteria was higher than that of archaea, while their richness and diversity varied widely across the sampling seasons. The α-diversity indices showed that high diversity was recorded in August, followed by September, June, and July in that order. The findings demonstrated that temperature, pH, P+, K+, NO3 -, and total dissolved solids (TDS) contributed majorly to the diversity observed in the microbial community. Multivariate analysis revealed significant spatial and temporal effects on β-diversity and salinity and alkalinity were the major drivers of microbial composition in Lake Magadi. Conclusions We provide insights into the relationships between microbial structure and geochemistry across various sampling sites in Lake Magadi.

show abstract

Characterization of Microbial Populations of Lake Van by 16s Metagenomics Study

Cited by 3 publications

References 27 publications

Microbial community of soda Lake Van as obtained from direct and enriched water, sediment and fish samples

Microbial community of soda Lake Van as obtained from direct and enriched water, sediment and fish samples

Spatiotemporal structure and composition of the microbial communities in hypersaline Lake Magadi, Kenya

Spatiotemporal structure and composition of the microbial communities in hypersaline Lake Magadi, Kenya

Contact Info

Product

Resources

About