Characterization of microbiota diversity of field‐collected <i>Haemaphysalis longicornis</i> (Acari: Ixodidae) with regard to sex and blood meals

Zhang, Ruiling; Huang, Zhongdong; Yu, Guoxian; Zhong, Zhang

doi:10.1002/jobm.201800372

Cited by 24 publications

(19 citation statements)

References 48 publications

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“…In the present study, we evaluated the effect of the two most common methods, one based on ethanol and the other on bleach, used to remove microbe contaminants present on tick cuticles. Although most previous studies on the internal microbiome of ticks commonly used an ethanol-based method [14, 17, 30, 42–46], we observed systematic differences between ethanol- and bleach-treated specimens. Both methods led to the detection of the B vitamin-providing symbiont Coxiella -LE in all A. cajennense ( s.s. ) samples, and of a putative pathogen Rickettsia in some of them, as expected from previous studies [50–53].…”

Section: Discussioncontrasting

confidence: 75%

“…ethanol- versus bleach-based methods) impact the difference in the diversity of internal microbial communities remains entirely unknown. It is noteworthy that studies using ethanol-based sterilization methods tend to show more diverse bacterial communities [14, 17, 30, 42–46] than studies using bleach-based sterilization methods [39, 47, 48]. However, this comparison is only partly relevant since these studies did not use standardized protocols: they differ in regard to the tick species examined, but also the stages and sexes of the ticks, the sampling localities, as well as the molecular and analytical approaches.…”

Section: Introductionmentioning

confidence: 99%

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Surface sterilization methods impact measures of internal microbial diversity in ticks

et al. 2019

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Background Ticks are obligate blood feeders transmitting major pathogens worldwide. Over the past few years, considerable research efforts have focused on the diversity, distribution and impact of gut and intracellular bacterial symbionts on tick development and tick-borne pathogen transmission. The study of this internal microbiome requires the use of a sterilization method to remove external (i.e. cuticular) microbes present on the tick’s surface and to avoid any further contamination. Several sterilization methods exist, including ethanol- or bleach-based treatments that are both effective in killing microbes but with different potential effects on DNA denaturation. Methods We examined how these different sterilization methods impact the measure of internal microbial diversity hosted by the Cayenne tick Amblyomma cajennense ( sensu stricto ). Bacterial barcoding investigations based on 16S rRNA gene sequences were conducted on two batches of 50 individuals each: Ticks of the first batch were sterilized with bleach diluted at 1% and the second batch with 70% ethanol. Tick external microbiome was also determined from cuticle smearing and water samples used for tick washing. Results Bacterial barcoding investigations showed major differences between ethanol- and bleach-treated specimens. Both methods led to the detection of major intracellular bacteria associated with A. cajennense ( s.s. ) but ethanol-treated ticks always harbored a higher bacterial diversity than bleach-treated ticks. Further examinations of tick gut and tick external microbiome revealed that ethanol-based surface sterilization method is inefficient to eliminate the DNA of external bacteria. Conclusions We herein provide evidence that studies investigating the internal microbiome of ticks should consider bleach as the gold standard to efficiently remove cuticular bacterial DNA. Indeed, this method does not impact the internal bacterial diversity hosted by ticks and is thus a better method than the ethanol-based one for studying the internal microbiome. Electronic supplementary material The online version of this article (10.1186/s13071-019-3517-5) contains supplementary material, which is available to authorized users.

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Section: Discussioncontrasting

confidence: 75%

Section: Introductionmentioning

confidence: 99%

Surface sterilization methods impact measures of internal microbial diversity in ticks

et al. 2019

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“…Some members of the intraspecific core bacteriome of these mosquitoes were not the most abundant within the community, and their relative abundance varied among locations. A similar pattern of b-diversity variation, provided by the less-frequent members, was observed in another insect, include the pine weevil, bark beetles, brown planthopper and Haemaphysalis longicornis (Durand et al, 2015;Berasategui et al, 2016;Zhang et al, 2018;Zhang et al, 2019).…”

Section: Discussionsupporting

confidence: 68%

Comparative Analysis of the Gut Microbiota of Adult Mosquitoes From Eight Locations in Hainan, China

Kang

Wang

et al. 2020

Front. Cell. Infect. Microbiol.

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The midgut microbial community composition, structure, and function of field-collected mosquitoes may provide a way to exploit microbial function for mosquito-borne disease control. However, it is unclear how adult mosquitoes acquire their microbiome, how the microbiome affects life history traits and how the microbiome influences community structure. We analyzed the composition of 501 midgut bacterial communities from field-collected adult female mosquitoes, including Aedes albopictus, Aedes galloisi, Culex pallidothorax, Culex pipiens, Culex gelidus, and Armigeres subalbatus, across eight habitats using the HiSeq 4000 system and the V3−V4 hyper-variable region of 16S rRNA gene. After quality filtering and rarefaction, a total of 1421 operational taxonomic units, belonging to 29 phyla, 44 families, and 43 genera were identified. Proteobacteria (75.67%) were the most common phylum, followed by Firmicutes (10.38%), Bacteroidetes (6.87%), Thermi (4.60%), and Actinobacteria (1.58%). The genera Rickettsiaceae (33.00%), Enterobacteriaceae (20.27%), Enterococcaceae (7.49%), Aeromonadaceae (7.00%), Thermaceae (4.52%), and Moraxellaceae (4.31%) were dominant in the samples analyzed and accounted for 76.59% of the total genera. We characterized the midgut bacterial communities of six mosquito species in Hainan province, China. The gut bacterial communities were different in composition and abundance, among locations, for all mosquito species. There were significant differences in the gut microbial composition between some species and substantial variation in the gut microbiota between individuals of the same mosquito species. There was a marked variation in different mosquito gut microbiota within the same location. These results might be useful in the identification of microbial communities that could be exploited for disease control.

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“…2011; Zhang et al . 2019). According to (Heath 2016), H. longicornis reduces the production of beef and dairy cattle in some regions of Australia and New Zealand.…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic comparison of cypermethrin‐susceptible and ‐tolerant Asian longhorned ticks (Haemaphysalis longicornis Neumann)

Moon

Kim

Lee

et al. 2021

Entomological Research

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The Asian longhorned tick (Haemaphysalis longicornis Neumann), native to northeast Asian regions, was recently confirmed to have invaded the USA. Although H. longicornis is a medically and economically important pest because it transmits various diseases to humans and livestock, its susceptibility to pesticides has not yet been fully investigated. Moreover, minimal genetic data on H. longicornis that could assist in its control are currently available. Therefore, in this study, to investigate the physiological response of H. longicornis to cypermethrin, a field population of H. longicornis was treated with various concentrations of cypermethrin (100–500 ppm), and the transcriptomes of a cypermethrin‐susceptible group (knock‐down at 200 ppm) and cypermethrin‐tolerant group (surviving at 500 ppm) were compared. In our analysis of the genes that were differentially expressed between the two groups of ticks, several genes involved in detoxification metabolism were upregulated in the cypermethrin‐tolerant group. Expression of genes encoding CYP450, GST, and ABC transporters were substantially higher in cypermethrin‐tolerant ticks than in the cypermethrin‐susceptible group. Given that CYP450, GST, and ABC transporters contribute to Phase I, II, and III of the detoxification metabolism process, respectively, our results suggest that H. longicornis responds physiologically to cypermethrin by altering the expression of various genes involved in detoxification metabolism, which in turn increases its tolerance to the cypermethrin.

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Characterization of microbiota diversity of field‐collected Haemaphysalis longicornis (Acari: Ixodidae) with regard to sex and blood meals

Cited by 24 publications

References 48 publications

Surface sterilization methods impact measures of internal microbial diversity in ticks

Surface sterilization methods impact measures of internal microbial diversity in ticks

Comparative Analysis of the Gut Microbiota of Adult Mosquitoes From Eight Locations in Hainan, China

Transcriptomic comparison of cypermethrin‐susceptible and ‐tolerant Asian longhorned ticks (Haemaphysalis longicornis Neumann)

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