Characterization of microheterogencity of human thyroglobulin from different thyroid disorders

Gärtner, Roland; Bechtner, G.; Greil, W.; Horn, K.; Pickardt, C. R.

doi:10.1530/acta.0.1090076

Cited by 10 publications

(4 citation statements)

References 12 publications

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“…Thyroglobulin was purified from a crude saline extract of a euthyroid goitre (14)(15)(16). Immediately after surgery the extract was processed in phosphate buffered saline: NaCl 150 mmol/1, phosphate buffer 10 mmol/1, pH 7.4, containing 2 mmol/1 diisopropylfluorophosphate or aprotinin (final concentration 0.5-3 X 10 6 KIU/1) (17).…”

Section: Thyroglobulin Preparationmentioning

confidence: 99%

Thyroglobulin Determined in Thyroid Fine Needle Aspiration Biopsies by Radial Immunodiffusion and Electroimmunodiffusion

Salabe¹,

Corvo²,

Lotz³

1996

CCLM

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Radial immunodiffusion and electroimmunodiffusion were used to measure thyroglobulin, the main component of thyroid colloid, in thyroid fine needle aspiration biopsies. A linear relationship was established between precipitation ring diameter and thyroglobulin concentration by radial immunodiffusion (0.5-3.0 g/1), and between "rocket" height and thyroglobulin concentration by electroimmunodiffusion (0.1-2.0 g/1). A nearly complete correlation was observed between the two methods (r = 0.97). In radial immunodiffusion the ring diameter is dependent on time of diffusion and on the antiserum concentration in the agar gel. In this study, the observation time was standardised at 48 h, and the rabbit anti-thyroglobulin serum concentration at 26 ml/1. The intrathyroidal concentration of thyroglobulin was determined by radial irrummodiffusion and the thyroid fine needle aspiration biopsy of 45 thyroid tumours with different cytological-laboratory-and clinical diagnoses. It was found that in colloid nodules or cysts thyroglobulin is markedly higher than in euthyroid nodular goitre (13.7 ± 11.9 g/1 vs 1.35 ± 0.8 g/l, p = 0.005).In conclusion radial immunodiffusion and electroimmunodiffusion are precise, easy to perform, low cost, non polluting methods, which do not require high sample dilution (in contrast, high sample dilution is necessary for measurement of thyroglobulin in thyroid fine needle aspiration biopsy by radial immunodiffusion). Measurement of thyroglobulin in thyroid fine needle aspiration biopsy provides a quantitative estimate of colloid, an important marker in the differential diagnosis of thyroid nodules.

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Section: Thyroglobulin Preparationmentioning

confidence: 99%

Thyroglobulin Determined in Thyroid Fine Needle Aspiration Biopsies by Radial Immunodiffusion and Electroimmunodiffusion

Salabe¹,

Corvo²,

Lotz³

1996

CCLM

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“…Separately, insulin and TSH shifted isoforms to acid pl; together their effects appeared t~ be additive, suggesting a supplementary increase of anionic charges. Gartner et al (1985) observed that isoforms of Tg were shifted to acid pl by the negative charges of iodide. All our experiments were performed in the absence of Nal and without the iodination conditions necessary to obtain iodinated Tg in this culture system (Gruffat et al, 1991(Gruffat et al, , 1992.…”

Section: Discussionmentioning

confidence: 99%

Hormonal regulation of some steps of thyroglobulin synthesis and secretion in bicameral cell culture

Desruisseau

Alquier

Depétris

et al. 1994

Journal Cellular Physiology

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Porcine thyroid cells were cultured for 15 days on porous bottom chambers with or without different mixtures of hormones added to serum-free basal medium. Assays with 10% serum were also performed for comparison with previously published results. The effects of the hormones, particularly insulin, TSH and hydrocortisone, were studied on total RNA content, thyroglobulin mRNA level, the amount of thyroglobulin secreted into the apical medium and on glycosylation. Insulin and TSH similarly increased the total RNA content, and their effects were additive. Thyroglobulin mRNA content was increased twofold by insulin and threefold by TSH. When they were added simultaneously, the maximal level of thyroglobulin mRNA was reached, showing that TSH and insulin effects on thyroglobulin gene expression were additive. Hydrocortisone alone did not modify total RNA or thyroglobulin mRNA content but the hormone amplified total RNA when insulin and TSH were present together. The basal level of thyroglobulin secreted into the apical medium was increased threefold by insulin and fourfold by TSH. The effects of these two hormones added together appeared to be additive. Hydrocortisone had no effect alone or even when combined with insulin or TSH. However, when the three hormones were added together, the hormonal response was amplified. TSH effect and insulin effect on the incorporation of 3H-mannose into thyroglobulin as well as on the anionic residue content of the molecule were additive.

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“…In addition, the lactoperoxidase method (17,19,21,22) and the iodogen technique (23,24) are enaployed. According to our data, the iodogen labeling procedure was slightly superior to chloramine T and lactoperoxidase; satisfactory 125 I incorporation, high specific activity and low nonspecific binding were obtained.…”

Section: Discussionmentioning

confidence: 99%

Radioimmunoassay for Serum Thyroglobulin Designed for Early Detection of Metastases and Recurrencies in the Follow-Up of Patients with Differentiated Thyroid Carcinoma

Falk¹,

Schmidt-Gayk²,

Hüfner³

1984

Clinical Chemistry and Laboratory Medicine

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Summary:A radioimniunoassay (RIA) for the measurement of thyroglobulin in human serum was developed and factors that influence sensitivity were investigated. In a comparison of 3 different labeling procedures (chloramine T, iodogen, lactoperoxidase) iodogen-prepared tracer proved to be slightly superior with respect to sensitivity and stability. The shelf life of the tracer was improved by a protein-enriched buffer, which serves äs a radical scavenger. The binding kinetics of tracer to antibody at different temperature ranges were examined, and the most rapid and complete binding was found at room temperature. For the preparation of Standard curves, several artificial media were compared with thyroglobulin-free serum. Second antibody Separation was investigated and optimized. By employing sequential Saturation, sensitivity of 0.75 g/l (Bö-3 SD) and 50% intercept of less than 5 g/l were achieved. The results of RIA measurements of thyroglobulin in 142 patients with papillary and follicular thyroid carcinoma after thyroidectomy and 131 I treatment were compared with 131 I whole-body scans. The results confirmed that serum thyroglobulin is an early indicator of recurrency. Radioimmunoassay für Thyreoglobulin im Serum zur Früherkennung von Rezidiven und Metastasen in der

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Characterization of microheterogencity of human thyroglobulin from different thyroid disorders

Cited by 10 publications

References 12 publications

Thyroglobulin Determined in Thyroid Fine Needle Aspiration Biopsies by Radial Immunodiffusion and Electroimmunodiffusion

Thyroglobulin Determined in Thyroid Fine Needle Aspiration Biopsies by Radial Immunodiffusion and Electroimmunodiffusion

Hormonal regulation of some steps of thyroglobulin synthesis and secretion in bicameral cell culture

Radioimmunoassay for Serum Thyroglobulin Designed for Early Detection of Metastases and Recurrencies in the Follow-Up of Patients with Differentiated Thyroid Carcinoma

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