Characterization of microRNAs during Embryonic Skeletal Muscle Development in the Shan Ma Duck

Abstract

Poultry skeletal muscle provides high quality protein for humans. Study of the genetic mechanisms during duck skeletal muscle development contribute to future duck breeding and meat production. In the current study, three breast muscle samples from Shan Ma ducks at embryonic day 13 (E13) and E19 were collected, respectively. We detected microRNA (miRNA) expression using high throughput sequencing following bioinformatic analysis. qRT-PCR validated the reliability of sequencing results. We also identified target prediction results using the luciferase reporter assay. A total of 812 known miRNAs and 279 novel miRNAs were detected in six samples; as a result, 61 up-regulated and 48 down-regulated differentially expressed miRNAs were identified between E13 and E19 (|log2 fold change| ≥ 1 and p ≤ 0.05). Enrichment analysis showed that target genes of the differentially expressed miRNAs were enriched on many muscle development-related gene ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, especially mitogen-activated protein kinase (MAPK) signaling pathways. An interaction network was constructed using the target genes of the differentially expressed miRNAs. These results complement the current duck miRNA database and offer several miRNA candidates for future studies of skeletal muscle development in the duck.

Developmental Alterations of Colonic microRNA Profiles Imply Potential Biological Functions in Kid Goats

“…Then, fifteen small libraries (five from each stage) were used for the Illumina/Solexa deep sequencing. Ligation of the total short RNAs (~18–26 nt in length), the reverse transcription, and PCR were performed according to previous work [ 21 ]. Finally, the amplification products were used directly for cluster generation and submitted to LC-Bio (Hangzhou, China) for the single-end sequencing on an Illumina Hiseq2500.…”

The Regulatory Mechanism of Feeding a Diet High in Rice Grain on the Growth and microRNA Expression Profiles of the Spleen, Taking Goats as an Artiodactyl Model

“…Then, ten small libraries (four samples from the NC group and six samples from the HC group) were used for Illumina/Solexa deep sequencing. Ligation of the total short RNAs (~18–26 nt in length), reverse transcription and PCR were performed according to previous work [ 20 ]. Finally, the amplification products were used directly for cluster generation and were then submitted to LC-Bio (Hangzhou, China) for single-end sequencing on an Illumina Hiseq2500.…”

Current Issues and Technical Advances in Cultured Meat Production: A Review

“…Muscle-specific microRNAs, called MyomiRs, are expressed in the muscle cells, although they are also expressed in several other tissues (McCarthy, 2008). Li et al found detected 279 novel miRNAs in the breast muscle of ducks, indicating the importance of miRNAs in muscle development and maturation (Li et al, 2020). Among these, miRNA-1 and miRNA-133 have been suggested to be crucial factors for duck skeletal muscle proliferation and differentiation.…”

“…Li et al. found detected 279 novel miRNAs in the breast muscle of ducks, indicating the importance of miRNAs in muscle development and maturation ( Li et al, 2020 ). Among these, miRNA-1 and miRNA-133 have been suggested to be crucial factors for duck skeletal muscle proliferation and differentiation.…”