Characterization of microRNAs in orange-spotted grouper ( Epinephelus coioides ) fin cells upon red-spotted grouper nervous necrosis virus infection

Chen, Wenjie; Yi, Lizhu; Feng, Suhua; Zhao, Lijuan; Li, Jun; Zhou, Meng; Liang, Rishen; Gu, Na; Wu, Zaohe; Tu, Jiagang; Lin, Li

doi:10.1016/j.fsi.2017.02.031

Cited by 42 publications

(19 citation statements)

References 73 publications

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“…MiR-22 negatively regulated type I IFN production by targeting MAVS [25]. A previous study has shown that miRNAs were associated with RGNNV infection [26], but the exact functions and regulation mechanisms of miRNAs during RGNNV infection are poorly understood. Herein, miR-202-5p expression was up-regulated in vivo and in vitro during RGNNV infection, suggesting its close association with RGNNV infection.…”

Section: Discussionmentioning

confidence: 99%

MiR-202-5p Inhibits RIG-I-Dependent Innate Immune Responses to RGNNV Infection by Targeting TRIM25 to Mediate RIG-I Ubiquitination

Liu

Jin

Zhang

et al. 2020

Viruses

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The RIG-I-like receptors (RLRs) signaling pathway is essential for inducing type I interferon (IFN) responses to viral infections. Meanwhile, it is also tightly regulated to prevent uncontrolled immune responses. Numerous studies have shown that microRNAs (miRNAs) are essential for the regulation of immune processes, however, the detailed molecular mechanism of miRNA regulating the RLRs signaling pathway remains to be elucidated. Here, our results showed that miR-202-5p was induced by red spotted grouper nervous necrosis virus (RGNNV) infection in zebrafish. Overexpression of miR-202-5p led to reduced expression of IFN 1 and its downstream antiviral genes, thus facilitating viral replication in vitro. In comparison, significantly enhanced levels of IFN 1 and antiviral genes and significantly low viral burden were observed in the miR-202-5p-/- zebrafish compared to wild type zebrafish. Subsequently, zebrafish tripartite motif-containing protein 25 (zbTRIM25) was identified as a target of miR-202-5p in both zebrafish and humans. Ectopic expression of miR-202-5p suppressed zbTRIM25-mediated RLRs signaling pathway. Furthermore, we showed that miR-202-5p inhibited zbTRIM25-mediated zbRIG-I ubiquitination and activation of IFN production. In conclusion, we demonstrate that RGNNV-inducible miR-202-5p acts as a negative regulator of zbRIG-I-triggered antiviral innate response by targeting zbTRIM25. Our study reveals a novel mechanism for the evasion of the innate immune response controlled by RGNNV.

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Section: Discussionmentioning

confidence: 99%

MiR-202-5p Inhibits RIG-I-Dependent Innate Immune Responses to RGNNV Infection by Targeting TRIM25 to Mediate RIG-I Ubiquitination

Liu

Jin

Zhang

et al. 2020

Viruses

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“…MiRNAs are also involved in host immune and stress response in eukaryotes, via regulating the expression of their target genes post-transcriptionally ( Achkar et al., 2016 ). As for marine organisms, miRNAs have been identified from many species such as fish ( Chen et al., 2017 ), crustaceans ( Zhou et al., 2015 ), echinoderms ( Wang et al., 2014 ; Mi et al., 2014 ), shellfish ( Picone et al., 2016 ), and cephalochordates ( Liao et al., 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Identification and characterization of microRNAs from the tube foot in the sea urchin Strongylocentrotus intermedius

Zhan

Cui

et al. 2018

Heliyon

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MicroRNAs (miRNAs) play critical roles in regulating many bio-processes of eukaryotes. The sea urchin Strongylocentrotus intermedius (an important fishery resource) is of great economic importance in Japan, North Korea, Russia, and China. In the current study, miRNAs of tube foot in S. intermedius were firstly identified and characterized. Data in this study can provide more genomic information for the further understanding of the complex regulation network in sea urchins and present a new way for monitoring the health status of cultured sea urchins.

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“…The expression of bacterial genes was normalized using 16s rDNA. In E. coioides , the expression of mRNA and lncRNA was normalized to β -actin , and the miRNA expression was normalized by using 5S rRNA gene ( 11 ). The 2 −ΔΔCt method was used to calculate the relative level of gene expression.…”

Section: Methodsmentioning

confidence: 99%

Integration of RNAi and RNA-seq Reveals the Immune Responses of Epinephelus coioides to sigX Gene of Pseudomonas plecoglossicida

et al. 2018

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Pseudomonas plecoglossicida is an important pathogen for aquaculture and causes high mortality in various marine fishes. Expression of sigX was found significantly up-regulated at 18°C than at 28°C, which was verified by quantitative real-time PCR (qRT-PCR). RNAi significantly reduced the content of sigX mRNA of P. plecoglossicida, whether in in vitro or in the spleen at all sampling time points. Compared with the wild-type strain, the infection of sigX-RNAi strain resulted in the onset time delay, and 20% reduction in mortality of Epinephelus coioides, as well as alleviates in the symptoms of E. coioides spleen. Compared with wild-type strain, the gene silence of sigX in P. plecoglossicida resulted in a significant change in transcriptome of infected E. coioides. The result of gene ontology and KEGG analysis on E. coioides showed that genes of serine-type endopeptidase and chemokine signaling pathway, coagulation and complement system, and intestinal immune network for IgA production pathway were mostly affected by sigX of P. plecoglossicida. Meanwhile, the immune genes were associated with different number of miRNA and lncRNA, and some miRNAs were associated with more than one gene at the same time. The results indicated that sigX was a virulent gene of P. plecoglossicida. The up-regulation of the immune pathways made E. coioides more likely to kill sigX-RNAi strain than the wild-type strain of P. plecoglossicida, while the immune genes were regulated by miRNA and lncRNA by a complex mode.

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Characterization of microRNAs in orange-spotted grouper ( Epinephelus coioides ) fin cells upon red-spotted grouper nervous necrosis virus infection

Cited by 42 publications

References 73 publications

MiR-202-5p Inhibits RIG-I-Dependent Innate Immune Responses to RGNNV Infection by Targeting TRIM25 to Mediate RIG-I Ubiquitination

MiR-202-5p Inhibits RIG-I-Dependent Innate Immune Responses to RGNNV Infection by Targeting TRIM25 to Mediate RIG-I Ubiquitination

Identification and characterization of microRNAs from the tube foot in the sea urchin Strongylocentrotus intermedius

Integration of RNAi and RNA-seq Reveals the Immune Responses of Epinephelus coioides to sigX Gene of Pseudomonas plecoglossicida

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