Characterization of microsatellite loci in <i>Brighamia insignis</i> and transferability to other genera in the Hawai‘ian lobelioid group

Fant, Jeremie B.; Fluckes, Mereida; James, Evana; Noble, Hilary; Wood, Jordan

doi:10.1002/aps3.11303

Cited by 2 publications

(4 citation statements)

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“…As the fourth accession (USBG2) died before we could extract DNA, we used progeny derived from a self of that plant (accession 2019‐000) to estimate paternal contribution. All samples were genotyped using microsatellite markers and protocols described in Fant et al ( 2019 ), which had already been tested for Hardy–Weinberg equilibrium and presence of null alleles. From this previous work, we identified a total of 11 primers that were reliable and polymorphic that would be useful for this study.…”

Section: Methodsmentioning

confidence: 99%

“…From this previous work, we identified a total of 11 primers that were reliable and polymorphic that would be useful for this study. They included eight primers (B05, B08, B43, B44, B46, B47, B51, B57) that were designed for B. insignis (Fant et al, 2019 ) and three primers (L23, L33, L34) that were originally designed for Lobelia villosa (Rock) H. St. John & Hosaka, which is not surprising as both species are part of the Hawaiian lobeliad clade (Barbará et al, 2007 ). The eight B. insignis primers were visualized using pre‐labeled forward primers with either WellRed Black (D2), Green (D3), or Blue (D4) fluorescent dye (Sigma‐Aldrich, St. Louis, Missouri, USA), while for L. villosa markers, the forward primer was modified at the 5′ end (5′‐CACGACGTTGTAAAACGAC‐3′) so they could be labeled separately (Schuelke, 2000 ).…”

Section: Methodsmentioning

confidence: 99%

“…Given that B. insignis is a paleotetraploid (Lammers, 1988 ), four of the primer pairs (B44, B47, B51, and L23) produced more than two bands. As the peaks were separated from other alleles by a large range (20–30 bp) and segregated independently (Fant et al, 2019 ), they were scored as independent loci.…”

Section: Methodsmentioning

confidence: 99%

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Supporting long‐term sustainability of ex situ collections using a pedigree‐based population management approach

et al. 2022

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Premise Living collections maintained for generations are at risk of diversity loss, inbreeding, and adaptation to cultivation. To address these concerns, the zoo community uses pedigrees to track individuals and implement crosses that maximize founder contributions and minimize inbreeding. Using a pedigree management approach, we demonstrate how conducting strategic crosses can minimize genetic issues that have arisen under current practices. Methods We performed crosses between collections and compared progeny fitness, including plant performance and reproductive health. We genotyped the progeny and parental accessions to measure changes in diversity and relatedness within and between accessions. Results The mean relatedness values among individuals within each accession suggest they are full siblings, demonstrating that there was high inbreeding and low diversity within accessions, although less so among accessions. Progeny from the wider crosses had increased genetic diversity and were larger and more fertile, while self‐pollinated accessions were smaller and less fertile. Discussion Institutions that hold exceptional species should consider how diversity is maintained within their collections. Implementing a pedigree‐based approach to managing plant reproduction ex situ will slow the inevitable loss of genetic diversity and, in turn, result in healthier collections.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Supporting long‐term sustainability of ex situ collections using a pedigree‐based population management approach

et al. 2022

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“…Considering the endangered status and continued rate of decline of G. remyi populations in the wild, the identification and maximization of genetic diversity within ex situ collections is a matter of urgency. The loss of genetic diversity within living plant collections can potentially be mitigated by developing a robust breeding program that incorporates genetic data from founding individuals into breeding decisions and ex situ management (Fant et al, 2016(Fant et al, , 2019Wood et al, 2020). Furthermore, utilizing methods focused on equalizing founder contribution and minimizing inbreeding can be used when propagating new plants to replenish aging accessions (Foster et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Assembling genetic structure of Gardenia remyi, a critically endangered tree endemic to the Hawaiian Islands

Hansen,

Opgenorth,

Flynn

et al. 2023

Conservat Sci and Prac

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Conservation and restoration planning of extremely rare species relies on an understanding of the genetic diversity and population dynamics within a species to overcome potential inbreeding depression. Nānū or Nāʻū (Gardenia remyi H. Mann.) is an endemic tree native to the Hawaiian Islands and is one of more than 200 endangered plant species in Hawaiʻi with less than 50 individuals remaining in the wild. Efforts to understand the genetic diversity and connectivity between wild populations are foundational to conservation management plans, however little is known of the population structure of the species. In this study we utilize double digest restriction‐site associated sequencing (ddRADSeq) on both historical herbarium specimens and samples from living ex situ collections to: (1) Test the hypothesis that we can capture genetic diversity in herbarium material of G. remyi using ddRADSeq, and (2) test the hypothesis that there are genetically distinct populations or subpopulation units among different Hawaiian islands. Usable sequencing data from thirty‐seven samples of herbarium specimens collected between 1952 and 2017 and twenty wild sourced living collection samples were obtained representing all four islands where G. remyi is known to occur. Phylogenetic and population structure analysis revealed a monophyletic ingroup and a clear division between G. remyi samples of the northern island of Kauaʻi and those from the more southeastern younger islands of Molokaʻi, Maui and Hawaiʻi islands. The Kauaʻi samples were further split into a subpopulation from Southern Kauaʻi and the subpopulations from Northern Kauaʻi. Some admixed samples were detected. Our results are consistent with subpopulations of G. remyi, which needs to be considered in future conservation planning and breeding efforts to minimize inbreeding depression.

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Characterization of microsatellite loci in Brighamia insignis and transferability to other genera in the Hawai‘ian lobelioid group

Cited by 2 publications

References 11 publications

Supporting long‐term sustainability of ex situ collections using a pedigree‐based population management approach

Supporting long‐term sustainability of ex situ collections using a pedigree‐based population management approach

Assembling genetic structure of Gardenia remyi, a critically endangered tree endemic to the Hawaiian Islands

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