Characterization of microsatellite loci in fulvous fruit bat <i>Rousettus leschenaulti</i>

Hua, Panyu; Chen, J. P.; Sun, Mingzhou; Liu, Bing; Zhang, S. Y.; Wu, Dengsheng

doi:10.1111/j.1471-8286.2006.01407.x

Cited by 15 publications

(12 citation statements)

References 10 publications

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“…For the amplification of the 20 nuclear microsatellite loci, the primers M3 (1, 6, 8, 120, 121), published in the study by Hua et al . (), and 66HDZ (20, 80, 82, 105, 106, 110, 117, 304, 327, 334, 340, 341, 343, 407, 413), from the study by Andrianaivoarivelo et al . (), were used (Table S2, Supporting Information).…”

Section: Methodsmentioning

confidence: 99%

Environmental margin and island evolution in Middle Eastern populations of the Egyptian fruit bat

et al. 2012

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Here, we present a study of the population genetic architecture and microevolution of the Egyptian fruit bat (Rousettus aegyptiacus) at the environmental margins in the Middle East using mitochondrial sequences and nuclear microsatellites. In contrast to the rather homogenous population structure typical of cave-dwelling bats in climax tropical ecosystems, a relatively pronounced isolation by distance and population diversification was observed. The evolution of this pattern could be ascribed to the complicated demographic history at higher latitudes related to the range margin fragmentation and complex geomorphology of the studied area. Lineages from East Africa and Arabia show divergent positions. Within the northwestern unit, the most marked pattern of the microsatellite data set is connected with insularity, as demonstrated by the separate status of populations from Saharan oases and Cyprus. These demes also exhibit a reduction in genetic variability, which is presumably connected with founder effects, drift and other potential factors related to island evolution as site-specific selection. Genetic clustering indicates a semipermeability of the desert barriers in the Sahara and Arabian Peninsula and a corridor role of the Nile Valley. The results emphasize the role of the island environment in restricting the gene flow in megabats, which is also corroborated by biogeographic patterns within the family, and suggests the possibility of nascent island speciation on Cyprus. Demographic analyses suggest that the colonization of the region was connected to the spread of agricultural plants; therefore, the peripatric processes described above might be because of or strengthened by anthropogenic changes in the environment.

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Section: Methodsmentioning

confidence: 99%

Environmental margin and island evolution in Middle Eastern populations of the Egyptian fruit bat

et al. 2012

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“…Tissue was stored in 95% alcohol and genomic DNA isolated as described above. PCR cocktails were set up following the protocol described in Hua et al . (2006), and a temperature gradient was used to optimize the primer annealing temperatures.…”

Section: Primer Sequences and Characteristics Of The Nine Microsatellmentioning

confidence: 99%

Development and characterization of novel microsatellite markers from the Chinese rufous horseshoe bat (Rhinolophus sinicus) with cross‐species amplification in closely related taxa

Zhang

Hua

et al. 2009

Molecular Ecology Resources

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We isolated nine polymorphic microsatellite markers from the Chinese rufous horseshoe bat (Rhinolophus sinicus) using an enriched library method. We assessed genetic polymorphism at these loci in 42 individuals from a single population. We recorded high genetic diversity with four to 17 alleles per locus, and estimated expected and observed heterozygosity values ranging from 0.492 to 0.910 and from 0.462 to 0.881, respectively. No locus departed from Hardy-Weinberg equilibrium following Bonferroni correction, and no linkage disequilibrium was detected. Most loci successfully cross-amplified congeneric species. These loci will be used to characterize phylogeographical history of Rhinolophus sinicus in China.

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“…Genomic DNA was extracted with the DNeasy TissueKit (Qiagen). The enrichment method for isolating CA repeats was based on Hua et al (2006). The genomic DNA extracted from S. kuhlii was digested with MboI (Takara) overnight, and the fragments between 300 and 900 bp were cut out from an agarose gel and purified with DNA purification kit (Takara).…”

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Isolation and characterization of 11 microsatellite loci in Scotophilus kuhlii (Lesser Asiatic Yellow House Bat)

Sun

Yu²,

Zhu³

et al. 2009

Conserv Genet

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We used enriched genomic library method to isolate and characterize 11 di-nucleotide microsatellite loci in the Lesser Asiatic Yellow House Bat, Scotophilus kuhlii. The polymorphism of these loci was tested on a natural population of 36 individuals from Hainan Province, South China. All loci revealed the polymorphism ranging from 5 to 18 alleles. The observed heterozygosity values were from 0.694 to 1.000 and expected heterozygosity values were from 0.558 to 0.929. No significant linkage disequilibrium was detected between any pairs of loci. These polymorphic markers will be used to assess population structure in S. kuhlii.

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Characterization of microsatellite loci in fulvous fruit bat Rousettus leschenaulti

Cited by 15 publications

References 10 publications

Environmental margin and island evolution in Middle Eastern populations of the Egyptian fruit bat

Environmental margin and island evolution in Middle Eastern populations of the Egyptian fruit bat

Development and characterization of novel microsatellite markers from the Chinese rufous horseshoe bat (Rhinolophus sinicus) with cross‐species amplification in closely related taxa

Isolation and characterization of 11 microsatellite loci in Scotophilus kuhlii (Lesser Asiatic Yellow House Bat)

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