Characterization of microsatellite loci in <i>Lychnis flos‐cuculi</i> (Caryophyllaceae)

Galeuchet, David J.; Husi, René; Perret, Catherine; Fischer, Markus; Gautschi, B.

doi:10.1046/j.1471-8286.2002.00294.x

Cited by 11 publications

(6 citation statements)

References 10 publications

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“…DNA was extracted from 10 mg of dry leaf material using the Dneasy 96 Plant Kit (QIAGEN, Hombrechtikon, Switzerland). We used three microsatellite markers developed for L. flos-cuculi (Galeuchet et al, 2002) and three markers developed for Silene latifolia (Moccia et al, 2009), a close relative of L. floscuculi. PCR was carried out as described in Aavik et al (2012).…”

Section: Methodsmentioning

confidence: 99%

The structural and functional connectivity of the grassland plant Lychnis flos-cuculi

2013

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Understanding the relationship between structural and functional connectivity is essential for successful restoration and conservation management, particularly in intensely managed agricultural landscapes. We evaluated the relationship between structural and functional connectivity of the wetland plant Lychnis flos-cuculi in a fragmented agricultural landscape using landscape genetic and network approaches. First, we studied the effect of structural connectivity, such as geographic distance and various landscape elements (forest, agricultural land, settlements and ditch verges), on gene flow among populations as a measurement of functional connectivity. Second, we examined the effect of structural graph-theoretic connectivity measures on gene flow among populations and on genetic diversity within populations of L. flos-cuculi. Among landscape elements, forests hindered gene flow in L. flos-cuculi, whereas gene flow was independent of geographic distance. Among the structural graphtheoretic connectivity variables, only intrapopulation connectivity, which was based on population size, had a significant positive effect on gene flow, that is, more gene flow took place among larger populations. Unexpectedly, interpopulation connectivity of populations, which takes into account the spatial location and distance among populations, did not influence gene flow in L. flos-cuculi. However, higher observed heterozygosity and lower inbreeding was observed in populations characterised by higher structural interpopulation connectivity. This finding shows that a spatially coherent network of populations is significant for maintaining the genetic diversity of populations. Nevertheless, lack of significant relationships between gene flow and most of the structural connectivity measures suggests that structural connectivity does not necessarily correspond to functional connectivity.

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Section: Methodsmentioning

confidence: 99%

The structural and functional connectivity of the grassland plant Lychnis flos-cuculi

2013

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“…As a measure of herbivory in the field, we used the average number of leaves damaged by herbivores in the field populations during the 3 years 2000–2002 (Galeuchet 2003), including the time when the initial selfing and outcrossing hand‐pollinations (see below) were conducted in 2000 (Perret 2003). Observed heterozygosity of the populations was determined with microsatellite markers based on 9–19 plants from each population (Galeuchet et al. 2002, 2005b).…”

Section: Methodsmentioning

confidence: 99%

Effects of experimental inbreeding on herbivore resistance and plant fitness: the role of history of inbreeding, herbivory and abiotic factors

2008

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Inbreeding is common in plant populations and can affect plant fitness and resistance against herbivores. These effects are likely to depend on population history. In a greenhouse experiment with plants from 17 populations of Lychnis flos-cuculi, we studied the effects of experimental inbreeding on resistance and plant fitness. Depending on the levels of past herbivory and abiotic factors at the site of plant origin, we found either inbreeding or outbreeding depression in herbivore resistance. Furthermore, when not damaged experimentally by snail herbivores, plants from populations with higher heterozygosity suffered from inbreeding depression and those from populations with lower heterozygosity suffered from outbreeding depression. These effects of inbreeding and outbreeding were not apparent under experimental snail herbivory. We conclude that inbreeding effects on resistance and plant fitness depend on population history. Moreover, herbivory can mask inbreeding effects on plant fitness. Thus, understanding inbreeding effects on plant fitness requires studying multiple populations and considering population history and biotic interactions.

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“…We screened all plants for variation at seven polymorphic microsatellite loci (Galeuchet et al . 2002b).…”

Section: Microsatellite Analysismentioning

confidence: 99%

“…Polymerase chain reaction (PCR) amplifications were performed in 10-µ L reaction volumes with 25 ng of genomic DNA, 0.5 µ m each of the fluorescence-labelled forward primer and of the reverse primer, and 0.5 units of the HotStar Taq Master Mix (QIAGEN). After a preliminary denaturation step at 95 ° C for 15 min, PCR amplification was performed for 30 cycles of 30 s denaturing at 95 ° C, 30 s of annealing at locus-specific temperatures (Galeuchet et al . 2002b) and 30 s of extension at 72 ° C, with a final 8 minute extension step at 72 ° C. We mixed 2 µ L of the PCR product with 10 µ L of a 75: 1 solution of formamide and GENESCAN-350(ROX) size standard (Applied Biosystems) solution.…”

Section: Microsatellite Analysismentioning

confidence: 99%

Microsatellite variation and structure of 28 populations of the common wetland plant, Lychnis flos‐cuculi L., in a fragmented landscape

2005

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Habitat fragmentation is known to cause genetic differentiation between small populations of rare species and decrease genetic variation within such populations. However, common species with recently fragmented populations have rarely been studied in this context. We investigated genetic variation and its relationship to population size and geographical isolation of populations of the common plant species, Lychnis flos-cuculi L., in fragmented fen grasslands. We analysed 467 plants from 28 L. flos-cuculi populations of different sizes (60 000-54 000 flowering individuals) in northeastern Switzerland using seven polymorphic microsatellite loci. Genetic differentiation between populations is small (F(ST) = 0.022; amova; P < 0.001), suggesting that gene flow among populations is still high or that habitat fragmentation is too recent to result in pronounced differentiation. Observed heterozygosity (H(O) = 0.44) significantly deviates from Hardy-Weinberg equilibrium, and within-population inbreeding coefficient F(IS) is high (0.30-0.59), indicating a mixed mating breeding system with substantial inbreeding in L. flos-cuculi. Gene diversity is the only measure of genetic variation which decreased with decreasing population size (R = 0.42; P < 0.05). While our results do not indicate pronounced effects of habitat fragmentation on genetic variation in the still common L. flos-cuculi, the lower gene diversity of smaller populations suggests that the species is not entirely unaffected.

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Characterization of microsatellite loci in Lychnis flos‐cuculi (Caryophyllaceae)

Cited by 11 publications

References 10 publications

The structural and functional connectivity of the grassland plant Lychnis flos-cuculi

The structural and functional connectivity of the grassland plant Lychnis flos-cuculi

Effects of experimental inbreeding on herbivore resistance and plant fitness: the role of history of inbreeding, herbivory and abiotic factors

Microsatellite variation and structure of 28 populations of the common wetland plant, Lychnis flos‐cuculi L., in a fragmented landscape

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