Characterization of microsatellite markers in peach [Prunus persica (L.) Batsch]

Sosinski, Bryon; Gannavarapu, M.; Hager, L. D.; Beck, Lien; King, Graham J.; Ryder, Carol D.; Rajapakse, S.; Baird, W. V.; Ballard, Robert; Abbott, Albert G.

doi:10.1007/s001220051499

Cited by 294 publications

(213 citation statements)

References 36 publications

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“…The standard protocol was modified by using 40 ml of NaOH and 95 ml of Tris buffer for each sample. Samples were genotyped at six microsatellite loci that were originally developed for Prunus avium and Prunus persica: UDP97-403 (Cipriani et al, 1999), P12A02 (Sosinski et al, 2000), BPPCT-002 (Dirlewanger et al, 2002), UDP96-005 and UDP98-410 (Schueler et al, 2003), and EMPaS06 (Vaughan and Russell, 2004). …”

Section: Molecular Analysesmentioning

confidence: 99%

Fine-scale spatial genetic dynamics over the life cycle of the tropical tree Prunus africana

et al. 2014

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Studying fine-scale spatial genetic patterns across life stages is a powerful approach to identify ecological processes acting within tree populations. We investigated spatial genetic dynamics across five life stages in the insect-pollinated and vertebratedispersed tropical tree Prunus africana in Kakamega Forest, Kenya. Using six highly polymorphic microsatellite loci, we assessed genetic diversity and spatial genetic structure (SGS) from seed rain and seedlings, and different sapling stages to adult trees. We found significant SGS in all stages, potentially caused by limited seed dispersal and high recruitment rates in areas with high light availability. SGS decreased from seed and early seedling stages to older juvenile stages. Interestingly, SGS was stronger in adults than in late juveniles. The initial decrease in SGS was probably driven by both random and non-random thinning of offspring clusters during recruitment. Intergenerational variation in SGS could have been driven by variation in gene flow processes, overlapping generations in the adult stage or local selection. Our study shows that complex sequential processes during recruitment contribute to SGS of tree populations.

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Section: Molecular Analysesmentioning

confidence: 99%

Fine-scale spatial genetic dynamics over the life cycle of the tropical tree Prunus africana

et al. 2014

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“…This method could certainly visualize the variations of both two S-alleles, which respective individuals possess, implying that null alleles are unlikely to be detected at the S-locus. Genotypes at 11 nuclear SSR loci, BPPCT005, 014, 026, 028, 034, 037, 038 and 040 (Dirlewanger et al, 2002), UDP96-001 (Cipriani et al, 1999), UDP98-412 (Testolin et al, 2000) and pchcms5 (Sosinski et al, 2000), were also scored using the method described by Kato et al (2011). Part of the genotypes had been already determined in our previous studies (Kato and Mukai, 2004;Kato et al, 2007Kato et al, , 2011.…”

Section: Genotyping Samples At the S-locus And Ssr Locimentioning

confidence: 99%

Impact of negative frequency-dependent selection on mating pattern and genetic structure: a comparative analysis of the S-locus and nuclear SSR loci in Prunus lannesiana var. speciosa

Kato

Saika²,

Junko³

et al. 2012

Heredity

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Mating processes of local demes and spatial genetic structure of island populations at the self-incompatibility (S-) locus under negative frequency-dependent selection (NFDS) were evaluated in Prunus lannesiana var. speciosa in comparison with nuclear simple sequence repeat (SSR) loci that seemed to be evolutionarily neutral. Our observations of local mating patterns indicated that male-female pair fecundity was influenced by not only self-incompatibility, but also various factors, such as kinship, pollen production and flowering synchrony. In spite of the mating bias caused by these factors, the NFDS effect on changes in allele frequencies from potential mates to mating pollen was detected at the S-locus but not at the SSR loci, although the changes from adult to juvenile cohorts were not apparent at any loci. Genetic differentiation and isolation-by-distance over various spatial scales were smaller at the S-locus than at the SSR loci, as expected under the NFDS. Allele-sharing distributions among the populations also had a unimodal pattern at the S-locus, indicating the NFDS effect except for alleles unique to individual populations probably due to isolation among islands, although this pattern was not exhibited by the SSR loci. Our results suggest that the NFDS at the S-locus has an impact on both the mating patterns and the genetic structure in the P. lannesiana populations studied.

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“…Genomic DNA from all the plant material was isolated according to Hormaza (2002). For the SSR analysis, the parental genotypes were initially screened with eight SSR loci previously developed in peach by Cipriani et al (1999) and Sosinski et al (2000), that have already been shown to be conserved in apricot (Hormaza, 2002) and Myrobalan (Serrano et al, 2002) genotypes: pchgms2, UDP96-003, UDP96-005, UDP96-008, UDP96-018, UDP98-405, UDP98-406 and UDP98-409. Three SSR loci (pchgms2, UDP96-003 and UDP96-008), polymorphic among the parental genotypes, were selected to confirm the hybrid origin of progeny.…”

Section: Progeny Characterizationmentioning

confidence: 99%

“…However, microsatellites or simple sequence repeats (SSRs) have become the markers of choice for fingerprinting and paternity analyses in most plant species (Gupta & Varshney, 2000) due to their high polymorphism, codominancy and reproducibility. Over 100 microsatellite markers have been so far reported in peach (Cipriani et al, 1999;Testolin et al, 2000;Sosinski et al, 2000;Aranzana et al, 2002Aranzana et al, , 2003Dirlewanger et al, 2002) and many of them are transferable to other related species (Hormaza, 2002;Wünsch & Hormaza, 2002).…”

Section: Introductionmentioning

confidence: 99%

Significant effect of accidental pollinations on the progeny of low setting Prunus interspecific crosses

et al. 2006

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SummaryThe incidence of fortuitous pollination on interspecific hybridizations of the plum rootstock Myrobalan with the apricot cultivars 'Moniquí' and 'Moniquí Borde' was assessed in this work. Progeny was originated through hand pollination of emasculated flowers of three Myrobalan clones, without bagging, in 1998 and 1999. Fruit set was low and variable among years (1.8-8.0%), but higher than the level of accidental pollination measured with emasculated and non-pollinated flowers (1.2%). Molecular characterization of the progeny was performed with three SSR markers showing that only 28% of the seedlings, obtained by in vitro germination and culture of immature embryos, were hybrids. This represents a lower percentage than expected, and is explained here by the low viability of hybrid embryos and seedlings. The use of molecular markers is discussed in terms of a convenient method for an early identification of putative hybrids in breeding programs with low setting crosses, where the proportion of non-hybrids is magnified.

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Characterization of microsatellite markers in peach [Prunus persica (L.) Batsch]

Cited by 294 publications

References 36 publications

Fine-scale spatial genetic dynamics over the life cycle of the tropical tree Prunus africana

Fine-scale spatial genetic dynamics over the life cycle of the tropical tree Prunus africana

Impact of negative frequency-dependent selection on mating pattern and genetic structure: a comparative analysis of the S-locus and nuclear SSR loci in Prunus lannesiana var. speciosa

Significant effect of accidental pollinations on the progeny of low setting Prunus interspecific crosses

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