Characterization of minisatellite sequences from <i>Oryza sativa</i>

Winberg, B.; Zhou, Z.; Dallas, John F.; McIntyre, C. Lynne; Gustafson, J. P.

doi:10.1139/g93-128

Cited by 42 publications

(20 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Thus, a relatively large degree of variation in the number of repeats was observed among loci of both GC-rich and AT-rich MSs contained in The range was indicated when the number of copies detected was two or more than two in each TE group Fig. 1 The En / Spm -like element TEOS6.2H containing minisatellite pOs6.2H reported by Winberg et al (1993). The sequences of the three members belonging to this TE group and the minisatellite pOs6.…”

Section: Variation In Repeat Numbers Among Mss In Dasheng and Bashomentioning

confidence: 92%

“…Winberg et al (1993) first isolated and characterized the GC-rich MS pOs6.2H from rice genomic DNA by using the human MS DNA 33.6 as a probe (Jeffreys et al 1985). This rice MS showed multi-banding patterns in Southern hybridization analysis at high stringency (Winberg et al 1993), and when the multiple loci were mapped, they were found to occur throughout the rice chromosomes (Gustafson and Yano 2000). These data strongly suggested that the MS pOs6.2H was also associated with a TE.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Role of transposable elements in the propagation of minisatellites in the rice genome

Inukai

2004

Mol Genet Genomics

View full text Add to dashboard Cite

A survey of minisatellites (MSs) in 5.3 Mb of randomly selected rice DNA sequences from public databases was carried out to clarify the role of transposable elements (TEs) in the dispersal of MSs in the rice genome. The estimated frequency of MSs in this sample was one per 23.4 kb, and this frequency is approximately equivalent to that of Class I microsatellites in the rice genome. Of the MSs in the 5.3-Mb sequence sample, 82% were found to be present in multiple copies in the rice genome, and all of these were a part of TE sequences. In this study at least 61 TE groups were identified as MS carriers. It was also shown that the GC-rich MS pOs6.2H, which was previously reported to be one of the interspersed MSs in the rice genome, is a component of an En/Spm-like element. These results indicate that the majority of MSs in the rice genome are maintained in TEs, and amplified and dispersed as components of the TEs. The G+C content of the multi-locus MS sequences reflected that of the TE sequences containing those MSs, but no obvious bias towards the high G+C content of DNA was observed. Single locus MSs also did not show any obvious bias towards the high G+C content of DNA in the rice genome. In this respect, the MSs in the rice genome are quite different from those in the human genome: in the latter, the majority of MSs show an obvious bias towards the high G+C content of DNA.

show abstract

Section: Variation In Repeat Numbers Among Mss In Dasheng and Bashomentioning

confidence: 92%

mentioning

confidence: 99%

Role of transposable elements in the propagation of minisatellites in the rice genome

Inukai

2004

Mol Genet Genomics

View full text Add to dashboard Cite

show abstract

“…Thus, in cereals, repetitive sequences can constitute more than 75% of the genome (Guidet et al 1991). In general, repetitive DNA sequences in plant genomes consist of satellites (Gupta et al 1990; Kamm et al 1995), minisatellites (Winberg et al 1993), microsatellites (Panaud et al 1995), and interspersed elements, including transposable elements such as retrotransposons (Flavell et al 1992;Voytas et al 1992; Leeton and Smyth 1993;Kolchinsky and Gresshoff1995). In recent years, repetitive DNA sequences have been cloned from various economically important plant species, characterized molecularly, and used as DNA markers.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of repetitive DNA sequences from Panax ginseng

Leung

2002

Mol Gen Genomics

View full text Add to dashboard Cite

Repetitive sequences constitute a significant component of most eukaryotic genomes, and the isolation and characterization of repetitive DNA sequences provide an insight into the organization and evolution of the genome of interest. We report the isolation and characterization of the major classes of repetitive sequences from the genome of Panax ginseng. The isolation of repetitive DNA from P. ginseng was achieved by the reannealing of chemically hydrolyzed (200 bp-1 kb fragments) and heat-denatured genomic DNA to low C(o)t value. The low C(o)t fraction was cloned, and fifty-five P. ginseng clones were identified that contained repetitive sequences. Sequence analysis revealed that the fraction includes repetitive telomeric sequences, species-specific satellite sequences, chloroplast DNA fragments and sequences that are homologous to retrotransposons. Two of the retrotransposon-like sequences are homologous to Ty1/ copia-type retroelements of Zea mays, and six cloned sequences are homologous to various regions of the del retrotransposon of Lilium henryi. The del retrotransposon-like sequences and several novel repetitive DNA sequences from P. ginseng were used to differentiate P. ginseng from P. quinquefolius, and should be useful for evolutionary studies of these disjunct species.

show abstract

“…Indeed, most of the reported repetitive elements are species-or genome-specific (Jiang et al 1996). In plant genomes, repetitive DNA sequences consist of satellites (Kamm et al 1995), minisatellites (Winberg et al 1993), microsatellites (Panaud et al 1995), and interspersed elements that include transposable elements, such as retrotransposons (Kolchinsky and Gresshoff 1995). Tandemly repeated sequences (satellite DNA) in plants normally have a characteristic chromosomal location: subtelomeric, interspersed or centromeric, with blocks of each motif present on some or all chro- J Appl Genet 48(3), 2007, pp.…”

Section: Introductionmentioning

confidence: 99%

Molecular analysis of a novel tandemly organized repetitive DNA sequence inCitrus limon (L.) Burm

et al. 2007

View full text Add to dashboard Cite

Repetitive sequences constitute a significant component of most eukaryotic genomes, and the isolation and characterization of repetitive DNA sequences provide an insight into the organization of the genome of interest. Here, we report the isolation and molecular analysis of a novel tandemly organized repetitive DNA sequence from the genome of Citrus limon. Digestion of C. limon DNA with Hinf I produced a prominent fragment of approximately 300 bp. Southern blotting revealed a ladder composed of DNA fragments that were multimers of the 300-bp Hinf I band. Thus, Hinf I digestion revealed a novel satellite, which we have called the C. limon satellite DNA 300 (CL300). Sequence analysis shows significant homology between a portion of the CL300 monomer and the transposase box of an En/Spm-like element. The CL300 satellite was also detected in grapefruit, sour orange, trifoliate orange and kumquat. These results suggest that the CL300 repeat is an ancient satellite, and we propose that a significant portion originated by amplification of a genomic region containing the En/Spm-like transposase element.

show abstract

Characterization of minisatellite sequences from Oryza sativa

Cited by 42 publications

References 27 publications

Role of transposable elements in the propagation of minisatellites in the rice genome

Role of transposable elements in the propagation of minisatellites in the rice genome

Isolation and characterization of repetitive DNA sequences from Panax ginseng

Molecular analysis of a novel tandemly organized repetitive DNA sequence inCitrus limon (L.) Burm

Contact Info

Product

Resources

About