Characterization of Molecular and Physiological Responses Under Water Deficit of Genetically Modified Soybean Plants Overexpressing the AtAREB1 Transcription Factor

Marinho, Juliane Prela; Kanamori, Norihito; Ferreira, L.C.; Fuganti-Pagliarini, Renata; Carvalho, Josirley de Fátima Corrêa; Freitas, Rafaela Alves; Marin, Silvana Regina Rockenbach; Rodrigues, Fabiana Aparecida; Mertz-Henning, L. M.; Farias, J. R. B.; Neumaier, N.; Oliveira, M. C. N. de; Marcelino-Guimarães, Francismar Corrêa; Yoshida, Takuya; Fujita, Yasunari; Yamaguchi-Shinozaki, Kazuko; Nakashima, Kenichiro; Nepomuceno, Alexandre Lima

doi:10.1007/s11105-015-0928-0

Cited by 26 publications

(35 citation statements)

References 66 publications

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“…The constitutive promoter 35S has been frequently used in the control of gene expression and, for a long time, was associated with plant growth retardation in the absence of stress (Liu et al 1998;Kasuga et al 1999;Kasuga et al 2004;Morran et al 2011). However, in soybean, under water deficit, Marinho et al (2015) worked with GM soybean plants overexpressing the transcription factor AtAREB1 under the control of CaMV35S promoter observed that, at the end of the experimental period, there were no differences regarding root dry matter, leaf blade dry matter, and total leaf area when plants were compared under well-watered and WD conditions, indicating that such a promoter can be successfully used to obtain GM soybean plants with no growth retardation.…”

Section: Discussionmentioning

confidence: 99%

Molecular, physiological, and agronomical characterization, in greenhouse and in field conditions, of soybean plants genetically modified with AtGolS2 gene for drought tolerance

et al. 2016

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Section: Discussionmentioning

confidence: 99%

Molecular, physiological, and agronomical characterization, in greenhouse and in field conditions, of soybean plants genetically modified with AtGolS2 gene for drought tolerance

et al. 2016

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“…Water deficit is considered the main abiotic stress that limits agricultural production worldwide (Inman-Bamber and Smith 2005). This stress often causes molecular, biochemical and physiological modifications (Marinho et al 2016;Boughalleb et al 2016;Pereira et al 2016) that negatively affect metabolism (Perlikowski et al 2016), reducing the growth and development (Mansori et al 2015), as well as the crop yield . Water limitations reduce the water potential (Fernandes-Silva et al 2016), lower the photosynthetic activity (Bertolli et al 2012), affect stomatal closing (Spinelli et al 2016), affect the accumulation of reactive oxygen species (Yi et al 2016), cause cell damages (Toscano et al 2016) and depending on the exposure time and intensity, can cause plant death (Chaves et al 2003;Shao et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Brassinosteroids improve photosystem II efficiency, gas exchange, antioxidant enzymes and growth of cowpea plants exposed to water deficit

Lima

Lobato

2017

Physiol Mol Biol Plants

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Water deficit is considered the main abiotic stress that limits agricultural production worldwide. Brassinosteroids (BRs) are natural substances that play roles in plant tolerance against abiotic stresses, including water deficit. This research aims to determine whether BRs can mitigate the negative effects caused by water deficiency, revealing how BRs act and their possible contribution to increased tolerance of cowpea plants to water deficit. The experiment was a factorial design with the factors completely randomised, with two water conditions (control and water deficit) and three levels of brassinosteroids (0, 50 and 100 nM 24-epibrassinolide; EBR is an active BRs). Plants sprayed with 100 nM EBR under the water deficit presented significant increases in U PSII , q P and ETR compared with plants subjected to the water deficit without EBR. With respect to gas exchange, P N , E and g s exhibited significant reductions after water deficit, but application of 100 nM EBR caused increases in these variables of 96, 24 and 33%, respectively, compared to the water deficit ? 0 nM EBR treatment. To antioxidant enzymes, EBR resulted in increases in SOD, CAT, APX and POX, indicating that EBR acts on the antioxidant system, reducing cell damage. The water deficit caused significant reductions in Chl a, Chl b and total Chl, while plants sprayed with 100 nM EBR showed significant increases of 26, 58 and 33% in Chl a, Chl b and total Chl, respectively. This study revealed that EBR improves photosystem II efficiency, inducing increases in U PSII , q P and ETR. This substance also mitigated the negative effects on gas exchange and growth induced by the water deficit. Increases in SOD, CAT, APX and POX of plants treated with EBR indicate that this steroid clearly increased the tolerance to the water deficit, reducing reactive oxygen species, cell damage, and maintaining the photosynthetic pigments. Additionally, 100 nM EBR resulted in a better dose-response of cowpea plants exposed to the water deficit.

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“…The isoline best performance, comparing to BR16 plants, is related to the decrease of stomatal conductance and transpiration. Those results suggest that the transgenic genotype improved capability to handle drought, without loss of yield ( Figure 4) (Marinho et al, 2016). In the coming years, Embrapa will make available to the soybean market, new cultivars with drought tolerance technology already inserted in the materials.…”

Section: Embrapa Technology For Drought Tolerancementioning

confidence: 93%

“…These genes allow the plant to receive and to identify environmental signals of abiotic stress, activating and regulating the genetic expression (Barbosa et al, 2012). Promising results are found in Arabidopsis (Fujita et al, 2005) and soybean (Marinho et al, 2016).…”

Section: Technologies In Trial Phasementioning

confidence: 99%

Soybean Crop: A Review on the Biotechnological Advances and Expectation for Modern Cultivars

Neto¹,

Nunes²,

Souza³

et al. 2020

JAS

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The soybean crop is extremely important for Brazilian agribusiness, generating millions of dollars in the country’s exports. Since its introduction in Brazil, soybean has undergone a process of technological modernization, receiving in the recent year’s new technologies that have provided a revolution in the production system, increasing mainly grain yield, as well as facilitated phytosanitary managements (pests, diseases, and weeds) and edaphoclimatic adaptation. Brazilian soybean producers yearn for new genotypes that make it easier to manage the crop and reduce input expenditures. New technologies are emerging or being improved to meet this demand. This review explores the technologies that are already available to soybean producers, inserted by the molecular breeding, such as Inox, Intacta, Cultivance, Libert Link and Enlist E3. It also brings the news that will be available to the market in a few years, such as Intacta 2 Xtend, Hb4, and other technologies to increase phenotyping and genotyping in breeding programs and insertion of characteristics to increase plant efficiency. Biotechnology is advancing at a frenetic pace and year after year, new techniques and tools are being made available for breeding programs around the world, which result in the production of new productive cultivars, conventional or transgenic, that are resistant to abiotic and biotic factors.

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Characterization of Molecular and Physiological Responses Under Water Deficit of Genetically Modified Soybean Plants Overexpressing the AtAREB1 Transcription Factor

Cited by 26 publications

References 66 publications

Molecular, physiological, and agronomical characterization, in greenhouse and in field conditions, of soybean plants genetically modified with AtGolS2 gene for drought tolerance

Molecular, physiological, and agronomical characterization, in greenhouse and in field conditions, of soybean plants genetically modified with AtGolS2 gene for drought tolerance

Brassinosteroids improve photosystem II efficiency, gas exchange, antioxidant enzymes and growth of cowpea plants exposed to water deficit

Soybean Crop: A Review on the Biotechnological Advances and Expectation for Modern Cultivars

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