Characterization of Molecular Forms of N-Terminal B-Type Natriuretic Peptide In Vitro

Ala-Kopsala, Minna; Moilanen, Anne-Mari; Rysä, Jaana; Ruskoaho, Heikki; Vuolteenaho, Olli

doi:10.1373/clinchem.2010.148775

Cited by 10 publications

(6 citation statements)

References 30 publications

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“…NT-proBNP concentrations were determined by immunoassay specific to human NT-proBNP10–29 as previously reported [12,13]. NT-proBNP is a product of the same precursor and therefore reflects the secretion of the biologically active peptide BNP.…”

Section: Methodsmentioning

confidence: 99%

Circulating N-terminal brain natriuretic peptide and cardiac function in response to acute systemic hypoxia in healthy humans

Heinonen

Luotolahti

Vuolteenaho

et al. 2014

Journal of Translational Medicine

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BackgroundAs it remains unclear whether hypoxia of cardiomyocytes could trigger the release of brain natriuretic peptide (BNP) in humans, we investigated whether breathing normobaric hypoxic gas mixture increases the circulating NT-proBNP in healthy male subjects.MethodsTen healthy young men (age 29 ± 5 yrs, BMI 24.7 ± 2.8 kg/m2) breathed normobaric hypoxic gas mixture (11% O2/89% N2) for one hour. Venous blood samples were obtained immediately before, during, and 2 and 24 hours after hypoxic exposure. Cardiac function and flow velocity profile in the middle left anterior descending coronary artery (LAD) were measured by Doppler echocardiography.ResultsArterial oxygen saturation decreased steadily from baseline value of 99 ± 1% after the initiation hypoxia challenge and reached steady-state level of 73 ± 6% within 20–30 minutes. Cardiac output increased from 6.0 ± 1.2 to 8.1 ± 1.6 L/min and ejection fraction from 67 ± 4% to 75 ± 6% (both p < 0.001). Peak diastolic flow velocity in the LAD increased from 0.16 ± 0.04 to 0.28 ± 0.07 m/s, while its diameter remained unchanged. In the whole study group, NT-proBNP was similar to baseline (60 ± 32 pmol/ml) at all time points. However, at 24 h, concentration of NT-proBNP was higher (34 ± 18%) in five subjects and lower (17 ± 17%), p = 0.002 between the groups) in five subjects than at baseline.ConclusionIn conclusion, there is no consistent increase in circulating NT-proBNP in response to breathing severely hypoxic normobaric gas mixture in healthy humans, a possible reason being that the oxygen flux to cardiac myocytes does not decrease because of increased coronary blood flow. However, the divergent individual responses as well as responses in different cardiac diseases warrant further investigations.

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Section: Methodsmentioning

confidence: 99%

Circulating N-terminal brain natriuretic peptide and cardiac function in response to acute systemic hypoxia in healthy humans

Heinonen

Luotolahti

Vuolteenaho

et al. 2014

Journal of Translational Medicine

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“…It has subsequently been further characterised with many antisera in single and two-site immunoassays [80] and by MS after tryptic digestion [76]. This 76 amino acid peptide is produced from its precursor peptide proBNP…”

Section: Probnp and Nt-probnp Circulating Formsmentioning

confidence: 99%

B-type Natriuretic Peptide circulating forms: Analytical and bioactivity issues

Yandle

Richards

2015

Clinica Chimica Acta

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“…With regard to oligomerization as the explanation for our results, we discussed various possibilities and found that oligomerization, which is not our original idea (3 ), appeared to be the one most consistent with our results (4 ). We are open to other explanations.…”

Section: In Replymentioning

confidence: 48%

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Ala-Kopsala¹,

Moilanen

Rysä

et al. 2011

Clinical Chemistry

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Characterization of Molecular Forms of N-Terminal B-Type Natriuretic Peptide In Vitro

Abstract: BACKGROUND:The heterogeneity of circulating peptides may influence the interpretation of results from N-terminal profragment of BNP (NT-proBNP) assays. Our objective was to characterize the heterogeneity for better usability of the assays.

Cited by 10 publications

References 30 publications

Circulating N-terminal brain natriuretic peptide and cardiac function in response to acute systemic hypoxia in healthy humans

Circulating N-terminal brain natriuretic peptide and cardiac function in response to acute systemic hypoxia in healthy humans

B-type Natriuretic Peptide circulating forms: Analytical and bioactivity issues

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