CHARACTERIZATION OF MOLLUSCICIDAL COMPONENT OF Moringa oleifera LEAF AND Momordica charantia FRUITS AND THEIR MODES OF ACTION IN SNAIL Lymnaea acuminata

Abstract

SUMMARYThe molluscicidal activity of the leaf powder of Moringa oleifera and lyophilized fruit powder of Momordica charantia against the snail Lymnaea acuminata was time and concentration dependent. M. oleifera leaf powder (96 h LC 50 : 197.59 ppm) was more toxic than M. charantia lyophilized fruit powder (96 h LC 50 : 318.29 ppm). The ethanolic extracts of M. oleifera leaf powder and Momordica charantia lyophilized fruit powder were more toxic than other organic solvent extracts. The 96 h LC 50 of the column purified fraction of M. oleifera leaf powder was 22.52 ppm, while that of M. charantia lyophilized fruit powder was 6.21 ppm. Column, thin layer and high performance liquid chromatography analysis show that the active molluscicidal components in M. oleifera leaf powder and lyophilized fruit of M. charantia are benzylamine (96 h LC 50 : 2.3 ppm) and momordicine (96 h LC 50 : 1.2 ppm), respectively. Benzylamine and momordicine significantly inhibited, in vivo and in vitro, the acetylcholinesterase (AChE), acid and alkaline phosphatase (ACP/ALP) activities in the nervous tissues of L. acuminata. Inhibition of AChE, ACP and ALP activity in the nervous tissues of L. acuminata by benzylamine and momordicine may be responsible for the molluscicidal activity of M. oleifera and M. charantia fruits, respectively.

Uso da planta "melão-de-são-caetano" (momordica charantia l.) no combate ao carrapato (rhipicephalus sanguineus) de cães – revisão de literatura

“…Avaliações fitoquímicas de extratos de M. charantia revelaram a presença de alcalóides, fenóis, catequinas, esteróides, terpenos e saponinas (CHEN et al, 2008;DALEFFI ZOCOLER et al, 2006;JUDD, 2009;RODRIGUES et al, 2010;UPADHYAY et al, 2013). Esses compostos podem ser caraterizados através de diversos métodos de extração de preparados e pelo fracionamento com solventes de diferentes polaridades, possibilitando inferir as possíveis classes de substâncias extraídas nas diferentes frações, de acordo com suas polaridades e solubilidades, que contribuem para as diversas atividades biológicas e farmacológicas da espécie vegetal (MEDEIROS;KANIS, 2010;OLIVEIRA, 2016;SHARAPIN, 2000;SIMÕES, 2007).…”

“…Estudos in vivo e in vitro demostraram que M. charantia possui atividade antibacteriana, antifúngica, repelente, larvicida, inseticida, moluscicida, anti-helmíntica e antiparasitária (AMORIM et al, 2011;BRASILEIRO et al, 2006;BRITO-JUNIOR et al, 2011;CELOTO et al, 2008;CORDEIRO et al, 2010;FARIA et al, 2009;JESUS et al, 2013;LIMA et al, 2017;MEDEIROS et al, 2013;MONTEIRO et al, 2011;NASCIMENTO et al, 2013;NETO et al, 2017;RODRIGUES et al, 2010;SANTIAGO et al, 2008;UPADHYAY et al, 2013;VENTUROSO et al, 2011). Celoto et al (2011) GALLO et al, 2002;ROEL, 2001).…”

Review of advances in the thin layer chromatography of pesticides: 2012–2014

“…The two compounds were found to significantly inhibit, in vivo and in vitro, the acetylcholinesterase and acid and alkaline phosphatase activity in the nervous tissues of Lymnaea acuminata, and these inhibitions may be responsible for the molluscicidal activity of the powders. [91] Purified active ingredient isolated from the commonly available botanical plant Ocimum sanctum L. was characterized and its potential for deployment in environmentfriendly disease-management strategy of staple food crop rice (Oryzae sativa L.) and rice-based crop groundnut (Arachis hypogea) in lieu of synthetic chemicals was assessed. Partial purification of active principal was conducted through silica gel column chromatography in polarity gradient systems followed by single zone isolation of active compound and mass collection through TLC.…”

Oral Supplementation with Benzylamine Delays the Onset of Diabetes in Obese and Diabetic db-/- Mice

“…In addition, various substituted benzylamine forms are present in shrubs, such as 2-acetyl-benzylamine in Adhatoda vasica [ 5 ], 4-hydroxybenzylamine in Cydonia vulgaris , [ 6 ], or tribenzylamine in hops [ 7 ]. Bza has also been evidenced in diverse medicinal plants, such as Lepidium meyenii (Maca) [ 8 , 9 ] and Moringa oleifera (Drumstick tree) [ 10 , 11 ]. Many parts of the tree Moringa oleifera are widely used for multiple applications, including human nutrition in developing countries [ 12 ], and recognized in folk medicine for their hypoglycemic activity.…”