Characterization of monoclonal antibodies against eosinophil chemotactic factors from young adult worms of Angiostrongylus cantonensis

Abstract

Two kinds of IgG1 monoclonal antibodies against eosinophil chemotactic factors (ECFs) derived from Angiostrongylus cantonensis young adult worms (YA) were established. Western blot analyses demonstrated that one monoclonal antibody recognized a 16.1 kD component of YA-whole worm extract, and the other an 85 kD component. These antibodies did not show any cross reactions against several helminth antigens, as assessed by ELISA. The chemotactic activity of YA-whole worm extract for guinea-pig-eosinophils was significantly inhibited by previous incubation of the extract with these monoclonal antibodies; the monoclonal antibody recognizing the 16.1 kD component inhibited 56-61% of the ECF activity whilst the antibody recognizing the 85 kD component inhibited 20-34% of the activity. The combination of both monoclonal antibodies showed a stronger inhibitory effect (71-81%) than either antibody alone. Similar magnitudes of inhibition were noted when the chemotactic activity for human eosinophils was measured using YA-whole worm extract preincubated with these monoclonal antibodies. Neither monoclonal antibodies inhibited the chemotactic activity of whole worm extracts of A. cantonensis first stage larvae, Metastrongylus apri adult worms, Spirometra erinacei plerocercoids and Fasciola sp. adult worms. These data suggest that the two monoclonal antibodies will be useful for the isolation and purification of ECF-YA as well as for elucidating the possible in vivo role of ECF-YA.

An Outbreak of Human Fascioliasis gigantica in Southwest China

“…The patients’ peripheral blood and stool samples were investigated over a few consecutive days. For the serological testing, we extracted the crude antigens from adult worms of F. hepatica and F. gigantica isolated from infected cattle and then connected the antigens to panels according to Ishida et al [17] and Chen et al [18]. Briefly, worms washed in phosphate-buffered saline (PBS, pH7.4) were individually homogenized with a tissue homogenizer for 3 minutes.…”

Eosinophilic Meningitis Due to Angiostrongylus cantonensis in Switzerland

“…However, only four to five antigen bands ( 29 kDa, 31 kDa, 55 kDa, 85-99 kDa, 200-204 kDa) can be considered as relevant for the serological diagnosis of A. cantonensis [11][12][13]. In our patient, four specific antigen bands (IgG) were seen.The specificity of A. cantonensis Western blot among helminthiasis is critical and cross-reactivity is inevitable.…”

A Protein Microarray for the Rapid Screening of Patients Suspected of Infection with Various Food-Borne Helminthiases

“…Whole worm antigens of A. cantonensis , P. westermani , T. spiralis and plerocercoids of Spirometra spp. were prepared following the standard protocol according to Ishida et al [45] and Chen et al [43]. Briefly, parasites were washed in phosphate-buffered saline (PBS), pH7.4 3 times, then homogenized in PBS with a tissue homogenizer (Bio-Gen PRO200 Homogenizer, PRO Scientific Inc., Oxford, CT, USA) for 3 minutes.…”