Characterization of mouse Bmp5 regulatory injury element in zebrafish wound models

Heller, Ian S.; Guenther, Catherine; Meireles, Ana M.; Talbot, William S.; Kingsley, David M.

doi:10.1016/j.bone.2021.116263

Cited by 6 publications

(3 citation statements)

References 97 publications

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“…In mature neuromasts in zebrafish (at 5 dpf), scRNA-seq data show that Bmp5 is expressed in neuromast hair cell progenitor populations and downregulated as hair cells differentiate (Lush et al, 2019). Furthermore, Bmp5 is among the genes upregulated in 5-dpf zebrafish neuromasts within one hour after neomycin-induced hair cell death (Jiang et al, 2014; Heller et al, 2022), and in the postnatal mouse cochlea after gentamycin-induced hair cell death (Bai et al, 2019). Hence, Bmp5 may be important for hair cell regeneration.…”

Section: Discussionmentioning

confidence: 99%

Two opposing roles for Bmp signalling in the development of electrosensory lateral line organs

Campbell,

Minařík,

Franěk

et al. 2024

Preprint

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The lateral line system enables all fishes and aquatic-stage amphibians to detect local water movement via mechanosensory hair cells in neuromasts, and many species to detect weak electric fields via electroreceptors (modified hair cells) in ampullary organs. Both neuromasts and ampullary organs develop from lateral line placodes. However, the molecular mechanisms underpinning ampullary organ formation are understudied relative to neuromasts, as electroreception was lost in the ancestors of zebrafish (teleosts) and Xenopus (frogs). We identified Bmp5 as a promising candidate via differential RNA-seq in an electroreceptive ray-finned fish, the Mississippi paddlefish (Polyodon spathula; Modrell et al., 2017, eLife 6: e24197). In an experimentally tractable relative, the sterlet sturgeon (Acipenser ruthenus), we found that Bmp5 and four other Bmp pathway genes are expressed in the developing lateral line, and that Bmp signalling is active. Furthermore, CRISPR/Cas9-mediated mutagenesis targeting Bmp5 in F0-injected sterlet embryos resulted in fewer ampullary organs. Conversely, when Bmp signalling was inhibited by DMH1 treatment shortly before the formation of ampullary organ primordia, supernumerary ampullary organs developed. These data suggest that Bmp5 promotes ampullary organ development, whereas Bmp signalling via another ligand(s) prevents their overproduction. Taken together, this demonstrates two opposing roles for Bmp signalling during ampullary organ formation.

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Section: Discussionmentioning

confidence: 99%

Two opposing roles for Bmp signalling in the development of electrosensory lateral line organs

Campbell,

Minařík,

Franěk

et al. 2024

Preprint

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“…However, BMP signaling of other cell types in response to injury has not been studied. Previous studies have proved a BMP5 gene regulates the injury element in zebrafish wound models; however, the mechanisms that lead to the reactivation of BMPs following injury are still unknown ( Heller et al, 2022 ). Therefore, further studies on the therapeutic effects of BMP signaling should be performed to investigate whether it can be targeted therapeutically in skin fibrotic diseases.…”

Section: Discussionmentioning

confidence: 99%

Dual-Action Icariin-Containing Thermosensitive Hydrogel for Wound Macrophage Polarization and Hair-Follicle Neogenesis

Teng

Zou

Liu

et al. 2022

Front. Bioeng. Biotechnol.

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Bone morphogenetic protein (BMP) pathway is essential for M2 macrophage polarization and hair-follicle neogenesis. Icariin, a flavonoid derived from Epimedium, is a mediator of the BMP pathway. Here, we develop a hydrogel formulation functionalized with icariin for regulation of macrophage polarization to accelerate wound healing and hair-follicle neogenesis. Compared to skin defects without icariin treatment, those treated with icariin+PEG hydrogel healed faster and had new hair follicles. Results in vivo showed that icariin+PEG hydrogel induced a higher level of M2 phenotypic transformation of macrophages. Moreover, icariin+PEG hydrogel significantly accelerated wound-repair process by reducing the invasion of inflammation, excessive deposition of collagen, immoderate activation of myofibroblasts, and increasing the regeneration of hair follicles. Furthermore, studies in vitro demonstrated that the icariin+PEG hydrogel induced macrophages to polarize to the M2 phenotype and dermal papilla cell to hair follicles. Finally, molecular analysis demonstrated that the icariin+PEG hydrogel increased the expression of BMP4 and Smad1/5 phosphorylation in skin wounds. These results demonstrate the therapeutic potential of icariin-containing thermosensitive hydrogels for inducing M2 macrophage polarization to accelerate wound healing and promote hair-follicle neogenesis by regulating the BMP pathway.

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“…Zebrafish (Danio rerio) have emerged as a powerful system for examining enhancers, owing to their genetic tractability, transparency in early developmental stages, and high fecundity (Kang et al, 2016;Yuan et al, 2018). Enhancers derived from other species, such as humans, mice, and sponges, can also direct conserved expression patterns in zebrafish, highlighting the usefulness of zebrafish for cross-species enhancer investigation (Heller et al, 2022;Perez-Rico et al, 2017;Wong et al, 2020;Yuan et al, 2018). Recent genome-wide analyses of zebrafish have rapidly expanded the list of enhancer candidates that may contribute to development and regeneration (Cao et al, 2022;Goldman et al, 2017;Kang et al, 2016;Quillien et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Regeneration and developmental enhancers are differentially compatible with minimal promoters

Begeman

Emery

Kurth

et al. 2022

Developmental Biology

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Characterization of mouse Bmp5 regulatory injury element in zebrafish wound models

Cited by 6 publications

References 97 publications

Two opposing roles for Bmp signalling in the development of electrosensory lateral line organs

Two opposing roles for Bmp signalling in the development of electrosensory lateral line organs

Dual-Action Icariin-Containing Thermosensitive Hydrogel for Wound Macrophage Polarization and Hair-Follicle Neogenesis

Regeneration and developmental enhancers are differentially compatible with minimal promoters

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