Characterization of mouse monoclonal antibodies specific for friend murine leukemia virus-induced erythroleukemia cells: Friend-specific and FMR-specific antigens

Chesebro, Bruce; Wehrly, Kathy; Cloyd, Miles W.; Britt, William J.; Portis, John L.; Collins, Joshua W.; Nishio, Jane

doi:10.1016/0042-6822(81)90619-x

Cited by 179 publications

(122 citation statements)

References 29 publications

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“…Clones were expanded in larger flasks. We confirmed the success of cloning by subjecting the light chains present in the tissue culture supernatants to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (18).…”

mentioning

confidence: 66%

Variable major proteins of Borrellia hermsii.

Barbour¹,

Tessier²,

Stoenner³

1982

The Journal of Experimental Medicine

183

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The reappearance of borreliae in a patient's blood during a second, third, or fourth febrile crisis of relapsing fever has suggested to students of this disease that these spirochetes undergo antigenic variation (1-4). Meleney summarized the state of knowledge of this phenemenon in 1928 (4): "At the time of the crisis which terminates the attack of fever, there is rapid agglutination and destruction of the spirochetes with the subsequent formation of immune bodies in the blood. These substances are specific for the strain of spirochetes which was present during the preceding attack, but have no influence on the spirochetes of the succeeding relapse. The spirochetes of the relapse give rise, in turn, to immune substances which are specific for them but not for the spirochetes of the first attack." Schuhardt and Wilkerson (5) showed in 1951 that these antigenically distinct relapse strains appeared after inoculations of rats with single organisms of Borrelia turicatae. Coffey and Eveland (6) subsequently identified by immunofluorescence three novel serotypes of Borrelia hermsii in the blood of rats experiencing relapses. One of us (H. G. Stoenner) has further defined this phenomenon of antigenic variation by studying B. hermsii, an agent of relapsing fever in North America, in mice. The use of serotype-specific antisera permitted identification of 24 different serotypes among the progeny of a single organism inoculated into a mouse (7).We are attempting to identify and characterize the variable antigens of B. hermsii. Although there has been considerable interest among biologists in similar phenomena shown by the salivarian trypanosomes (8-12), relatively little is known of borrelial antigens, their locations in the cells, and the mechanism of antigenic variation. Using both polyclonal antisera and monoclonal antibodies, we have identified in whole cell lysates of B. hermsii an abundant protein that is serotype specific. Materials and MethodsOrganisms and Culture Conditions. The origin of the B. hermsii strain used in these studies has been described (13,14). It was designated strain HS1. Details of the strategy and methods used for identification and isolation of relapse serotypes is described elsewhere (7). In summary, HS1 was cloned by limiting dilution in Swiss mice of the Rocky Mountain Laboratories stock (RML). 1 Spirochetes were enumerated by dark-field microscopy (14). Preliminary experiments had shown that one borrelia inoculated intraperitoneally was sufficient to produce spiroche-1 Abbreviations used in this paper: BSA, bovine serum albumin; C, culture; FA, direct immunofluorescence assay; FCS, fetal calf serum; IFA, indirect immunofluorescence assay; NCP, nitrocellulose; PBS, phosphatebuffered saline, pH 7.4; PBS/Mg, phosphate-buffered saline with 5 mM MgCI2; RML, Rocky Mountain Laboratories; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis; Tris, tris-(hydroxymethyl)aminomethane; TSGAN, 50 mM Tris, pH 7.4, 150 mM NaCI, 5 mM EDTA, 0.25% gelatin, 0.05% sodium azide, and 0.05% Nonidet ...

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mentioning

confidence: 66%

Variable major proteins of Borrellia hermsii.

Barbour¹,

Tessier²,

Stoenner³

1982

The Journal of Experimental Medicine

183

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“…2C). mAb 34, is a mouse IgG2b specific for the glycoGag protein (45), which is expressed on infected cells but present at extremely low levels in virion particles (38). For in vivo treatments, 42 g of mAb 34 was injected i.p., resulting in a mean binding titer of 1:640, equivalent to the titer after vaccination with live attenuated virus (Fig.…”

Section: Methodsmentioning

confidence: 99%

“…To determine whether virus-neutralizing antibodies could substitute for the absence of B cells in B6.UMT mice, passive transfer experiments were performed using virus-neutralizing mAb 48 specific for the FV Env protein (45,49). Passive transfer of mAb 48 has been shown to reduce viremia levels in vivo without inducing antibody-dependent cellular cytotoxicity in infected cells (50).…”

Section: Figmentioning

confidence: 99%

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Essential role for virus-neutralizing antibodies in sterilizing immunity against Friend retrovirus infection

Messer

Dittmer

Peterson

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

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“…Progress in the analysis of primary structures of polypeptides and nucleic acids, and in the synthesis of oligopeptides, has made the use of synthetic peptides a new promising approach to vaccine development, Studies with monoclonal antibodies show that binding of antibodies to a single epitope may be sufficient to neutralize viral infectivity (Chesebro et al, 1981). Consequently, it should be possible to use a single synthetic epitope as an effective vaccine.…”

Section: Introductionmentioning

confidence: 99%

Synthetic Vaccines against Friend Murine Leukaemia Virus-induced Erythroleukaemia: in vivo and in vitro Studies with Synthetic Oligopeptides and Sequence-specific Antisera

Bayer¹,

Hunsmann²

1987

Journal of General Virology

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SUMMARYBiological activities of antisera against synthetic oligopeptides were examined. The peptide antisera were directed against amino acids 6 to 12 (pepl), 124 to 131 (pep2), 256 to 262 (pep3), 283 to 290 (pep4) and 434 to 441 (pep5) of the viral envelope glycoprotein (gp70). Peptide-specific antisera did not neutralize viral infectivity. However, antibodies to pep4 and pep5, which bound to the hydrophobic part of gp70, mediated the complement-dependent lysis of Friend murine leukaemia virus (FLV)-infected cells. STU mice were immunized against FLV-induced erythroleukaemia with synthetic oligopeptides. Vaccines containing only one of these peptides (single-peptide vaccines) as well as multi-peptide vaccines containing pepl, -4, -5 or pepl, -2, -3, -4, -5 were used. Whereas the immunizations with single-peptide vaccines did not protect the immunized mice from FLV-induced erythroleukaemia, multi-peptide vaccines enhanced the survival rate and incubation period after FLV challenge. These results revealed that immunological reactions distinct from neutralizing antibodies can be evoked by immunization with synthetic peptides and can confer limited protection against FLV-induced erythroleukaemia.

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Characterization of mouse monoclonal antibodies specific for friend murine leukemia virus-induced erythroleukemia cells: Friend-specific and FMR-specific antigens

Cited by 179 publications

References 29 publications

Variable major proteins of Borrellia hermsii.

Variable major proteins of Borrellia hermsii.

Essential role for virus-neutralizing antibodies in sterilizing immunity against Friend retrovirus infection

Synthetic Vaccines against Friend Murine Leukaemia Virus-induced Erythroleukaemia: in vivo and in vitro Studies with Synthetic Oligopeptides and Sequence-specific Antisera

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