Characterization of MPF activation by okadaic acid in Xenopus oocyte

Rime, Hélène; Huchon, Denise; Jessus, Catherine; Goris, Jozef; Merlevede, Wilfried; Ozon, René

doi:10.1016/0922-3371(90)90023-p

Cited by 62 publications

(35 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MPF activity depends on the stability of cyclin B . Moreover, OA is known to indirectly activate MPF in Xenopus oocytes and extracts (Goris et al, 1989;Rime et al, 1990;Jessus et al, 1991;Lorca et al, 1991). Therefore, the sensitivity of EDEN-dependent deadenylation to OA could be due, in part, to activation of MPF.…”

Section: Mpf Pathway Does Not Inhibit Eden-dependent Deadenylationmentioning

confidence: 99%

Regulation of EDEN-dependent deadenylation of Aurora A/Eg2-derived mRNA via phosphorylation and dephosphorylation inXenopus laevisegg extracts

Détivaud

Pascreau

Karaı̈skou

et al. 2003

Journal of Cell Science

View full text Add to dashboard Cite

Deadenylation is an intimate part of the post-transcriptional regulation of maternal mRNAs in embryos. EDEN-BP is so far the only known member of a complex regulating the deadenylation of maternal mRNA in Xenopus laevis embryos in a manner that is dependent on the 3′-untranslated region called EDEN (embryo deadenylation element). In this report, we show that calcium activation of cell-free extracts triggers EDEN binding protein(EDEN-BP) dephosphorylation and concomitant deadenylation of a chimeric RNA bearing Aurora A/Eg2 EDEN sequence. Deadenylation of mRNA deprived of EDEN sequence (default deadenylation) does not change with egg activation. Kinase and phosphatase inhibitors downregulate EDEN-dependent deadenylation but they do not substantially influence default deadenylation. Using indestructibleΔ90 cyclin B to revert interphase extracts to the M-phase, we show that modulation of EDEN-dependent deadenylation is independent of M-phase promoting factor (MPF) activity. These results suggest that the increase in EDEN-dependent deadenylation following egg activation is achieved, at least partially, via dephosphorylation and/or phosphorylation of regulatory proteins, including EDEN-BP dephosphorylation. This regulation proceeds in a manner independent from MPF inactivation.

show abstract

Section: Mpf Pathway Does Not Inhibit Eden-dependent Deadenylationmentioning

confidence: 99%

Regulation of EDEN-dependent deadenylation of Aurora A/Eg2-derived mRNA via phosphorylation and dephosphorylation inXenopus laevisegg extracts

Détivaud

Pascreau

Karaı̈skou

et al. 2003

Journal of Cell Science

View full text Add to dashboard Cite

show abstract

“…Microinjection of 50 nl of 10 Ϫ5 M OA into prophase oocytes induces rapid p34 cdc2 activation (Goris et al, 1989) and a subsequent cytologically abnormal GVBD (Rime et al, 1990). It has been proposed that microinjected OA, by promoting phosphorylation/activation of Cdc25, initiates the positive feedback loop that controls activation of p34 cdc2 in ovo (Izumi and Maller, 1993).…”

Section: P21 Cip1 Interacts With P34 Cdc2 /Cyclin Complexes and Inhibmentioning

confidence: 99%

Two Distinct Mechanisms Control the Accumulation of Cyclin B1 and Mos inXenopusOocytes in Response to Progesterone

Frank-Vaillant

Jessus

Ozon

et al. 1999

MBoC

Self Cite

View full text Add to dashboard Cite

Progesterone-induced meiotic maturation of Xenopus oocytes requires the synthesis of new proteins, such as Mos and cyclin B. Synthesis of Mos is thought to be necessary and sufficient for meiotic maturation; however, it has recently been proposed that newly synthesized proteins binding to p34 cdc2 could be involved in a signaling pathway that triggers the activation of maturation-promoting factor. We focused our attention on cyclin B proteins because they are synthesized in response to progesterone, they bind to p34 cdc2 , and their microinjection into resting oocytes induces meiotic maturation. We investigated cyclin B accumulation in response to progesterone in the absence of maturation-promoting factor-induced feedback. We report here that the cdk inhibitor p21 cip1 , when microinjected into immature Xenopus oocytes, blocks germinal vesicle breakdown induced by progesterone, by maturation-promoting factor transfer, or by injection of okadaic acid. After microinjection of p21 cip1 , progesterone fails to induce the activation of MAPK or p34 cdc2 , and Mos does not accumulate. In contrast, the level of cyclin B1 increases normally in a manner dependent on down-regulation of cAMP-dependent protein kinase but independent of cap-ribose methylation of mRNA.

show abstract

“…OA was referred to induce the maturation of oocytes maintained arrested in dictyotene. After OA injection, immature oocytes rapidly showed NEBD, lamin depolymerization, and chromosome condensation, accompanied with a rapid appearance of MPF activity, and with a burst of protein phosphorylation in Xenopus and mouse [21,22]. These experiments also showed a full biological effectivity (tested by injection into other oocytes) of MPF appeared after the OA treatment either in oocytes with completely blocked proteosynthesis, indicating that preformed inactive MPF existed in their ooplasms.…”

Section: Okadaic Acid Treatment-induced Chromosome Condensationmentioning

confidence: 50%

“…[5,8]), all studies were focused to oocytes, polar bodies and/or D1 embryos (zygotes) (e.g. [1,4,9,11,12,15,[21][22][23]25]). We speculate that ooplasmic store of plenty of kinds of molecules and organelles may play a certain role.…”

Section: Okadaic Acid Treatment-induced Chromosome Condensationmentioning

confidence: 99%

“…(iii) A decade ago, the maturation stimulating effect of okadaic acid on animal oocytes was described, and MPF was shown to play the key role in this process, which involves chromosome condensation [21,22]. Several experiments based on chromosome condensation induced by okadaic acid treatment (OAT) were performed in mammalian oocytes and D1 embryos (zygotes) [9,25].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Experimental attempts to extend the current preimplantation genetic diagnosis with individual karyotypization of human blastomeres

et al. 2001

View full text Add to dashboard Cite

-The drug-induced chromosome condensation using okadaic acid, a potent protein phosphatase inhibitor, was studied in day 1 to day 4 (D1-D4) spare human preimplantation embryos. In order to obtain cells for genetic tests, we developed a modified displacement blastomere biopsy method. During the okadaic acid treatment, approximately 40% of biopsied cells were lost due to heavy changes of the plasma membrane; this detrimental effect of okadaic acid differed markedly with the respect to the age of embryos. In comparison with the natural embryonic mitotic index, day 1 and day 2 embryonic cells gave increased yields of chromosome spreads (up to 51% of the initial D1-D2 cell number); on days 3 and 4 we were not able to obtain from surviving cells more than 31% blastomeres with condensed chromosomes (9% of total D3-D4 cell number). All chromosome spreads were successfully used for recycling in G-banding and subsequent FISH analysis. assisted reproduction / blastomere biopsy / chromosome aberration / fluorescent in situ hybridization (FISH) / G-banding / human early embryo / karyotype / okadaic acid / preimplantation genetic diagnosis Résumé -Effort expérimental orienté vers l'élargissement du diagnostic génétique de préimplantation de la caryotypisation de différents blastomères humains. Nous avons étudié la condensation des chromosomes de différentes cellules qui étaient isolées des embryons humains aberrants de préimplantation agées de 1-4 jours (D1-D4), provoquée par l'acide okadaic -un inhibiteur fort des phosphatases de protéines. Nous avons utilisé la méthode modifiée de la biopsie d'un blastomère. Cca 40 % de cellules a disparu à cause de grandes modifications de la membrane cytoplasmique au cours de l'action de l'acide okadaic. Chez les cellules des embryons de 1 et de 2 jours, nous avons obtenu le nombre élevé de chromosomes (jusqu'à 51 % du nombre initial de cellules). En ce qui concerne les cellules de 3 et 4 jours, nous n'avons réussi à obtenir que 31 % de blastomères avec Reprod. Nutr. Dev. 41 (2001) [91][92][93][94][95][96][97][98][99][100][101][102][103][104][105][106] 91

show abstract

Characterization of MPF activation by okadaic acid in Xenopus oocyte

Cited by 62 publications

References 47 publications

Regulation of EDEN-dependent deadenylation of Aurora A/Eg2-derived mRNA via phosphorylation and dephosphorylation inXenopus laevisegg extracts

Regulation of EDEN-dependent deadenylation of Aurora A/Eg2-derived mRNA via phosphorylation and dephosphorylation inXenopus laevisegg extracts

Two Distinct Mechanisms Control the Accumulation of Cyclin B1 and Mos inXenopusOocytes in Response to Progesterone

Experimental attempts to extend the current preimplantation genetic diagnosis with individual karyotypization of human blastomeres

Contact Info

Product

Resources

About