Characterization of mucin in whole-gut lavage fluid obtained from patients with inflammatory bowel disease

Abstract

Whole-gut lavage fluid, collected by administering an electrolyte lavage solution orally, was found to be an excellent and easily collectable source of abundant mucin. Furthermore, the biochemical features of the mucin from patients with ulcerative colitis and Crohn's disease were investigated. The mucin was separated into four fractions by Sepharose CL-4B, Sepharose CL-2B, and DEAE Sephacel chromatography. Compared with healthy subjects, the total yields of mucin from ulcerative colitis patients were low due to a deficiency of neutral mucin, whereas those from Crohn's disease patients were high, which was attributable mainly to high-molecular-weight mucin. The fucose and sulfate contents were low in ulcerative colitis, but only the former was low in Crohn's disease. The different biochemical features of the mucin obtained from whole gut lavage fluid appear to reflect mucosal pathological changes associated with inflammatory bowel disease.

Aluminum Is a Potential Environmental Factor for Crohn's Disease Induction

“…Concerning human absorption, Al is poorly absorbed but many luminal compounds influence bioavailability determined by its solubility and diffusibility. One such factor is intestinal mucin, which is altered in CD patients 27,72 . Al is absorbed along the gastrointestinal tract, which is the target organ for CD.…”

“…One such factor is intestinal mucin, which is altered in CD patients. 27,72 Al is absorbed along the gastrointestinal tract, which is the target organ for CD. In fact, once absorbed, it is transported by transferrin at the sites left vacant by iron.…”

Thickness and continuity of the adherent colonic mucus barrier in active and quiescent ulcerative colitis and Crohn’s disease

“…showed that the thickness of the adherent mucus layer was reduced in UC compared with the normal rectum but in contrast, the thickness of the mucus layer in the rectum was increased in CD (20). These results were mirrored by the yields of mucin obtained from whole‐gut lavage, which were low in UC but high in CD (22). Recently, using preservation of mucus in Carnoy’s solution Swidsinski et al.…”

“…In severe UC the mucus layer and goblet cell density were significantly reduced and likely to contribute to the symptomatology of the disease. of mucin obtained from whole-gut lavage, which were low in UC but high in CD (22). Recently, using preservation of mucus in Carnoy's solution Swidsinski et al also demonstrated a reduction in the mucus layer in UC (23).…”

Compensatory response of colon tissue to dextran sulfate sodium-induced colitis

“…Although this result is very interesting, it can be thought to reflect goblet cell depletion and recovery. Saitoh et al 8 reported the measurement of mucin in whole gut lavage fluid from patients with inflammatory bowel disease. It is doubtful whether the recovery of mucin in the lavage fluid is perfect, and it is uncertain whether the origin of the mucin is from the inflammatory or the normal mucosa.…”