Characterization of multicopy suppressor genes that complement a defect in the Wis1-Sty1 MAP kinase cascade involved in stress responses in Schizosaccharomyces pombe.

Yamada, H.; Ohmiya, Ryusuke; Yamamoto, Eiji; Aiba, Hirofumi; Mizuno, Takeshi

doi:10.2323/jgam.43.209

Cited by 11 publications

(8 citation statements)

References 20 publications

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“…First, we analyzed the suppressing ability of some plasmid clones in our laboratory stock to determine whether they could improve the reduced viability of the Dsty1 mutant. Previously we screened an S. pombe genomic library for a plasmid that could suppress the heat-stress sensitivity of the Dsty1 mutant (Yamada et al, 1997). Among the suppressing plasmids, pSDS23, which encodes protein that functions as a multicopy suppressor for mutations of the PP1 protein phosphatase and the 20S cyclosome/anaphase-promoting complex (APC), and pHXK2, which encodes hexokinase 2, were revealed not to suppress the short-lived phenotype of the Dsty1 mutant (data not shown).…”

Section: Resultsmentioning

confidence: 99%

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A novel gene, ecl1+, extends the chronological lifespan in fission yeast

Ohtsuka

Mita

Ogawa

et al. 2008

FEMS Yeast Research

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We have identified a novel gene from Schizosaccharomyces pombe that we have named ecl1(+) (extender of the chronological lifespan). When ecl1(+) is provided on a high-copy number plasmid, it extends the viability of both the Deltasty1 MAP kinase mutant and the wild-type cells after entry into the stationary phase. ecl1(+) encodes an 80-amino acid polypeptide that had not been annotated in the current database. The ecl1(+)-mRNA increases transiently when the growth phase is changed from the log phase to the stationary phase. The Ecl1 protein is localized in the nucleus. Calorie restriction extends the chronological lifespan of wild-type and Deltaecl1 cells but not ecl1(+)-overproducing cells. The Deltapka1 mutant shows little, if any, additional extension of viability when Ecl1 is overproduced. The ste11(+) gene that is negatively controlled by Pka1 is up regulated when Ecl1 is overproduced. From these results we propose that the effect of Ecl1 overproduction may be mainly linked to and negatively affects the Pka1-dependent pathway.

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Section: Resultsmentioning

confidence: 99%

“…In brief, the 1.34-kb DNA fragment shown in Fig. 2 was cloned in the vector plasmid pLDblet (Yamada et al, 1997). To introduce a stop codon into Ecl1 protein, site-directed mutagenesis was performed on the pSht3 plasmid using the site-directed mutagenesis primer: CTGCCATCCTAAGTGGTGGCT.…”

Section: Plasmid Constructionmentioning

confidence: 99%

A novel gene, ecl1+, extends the chronological lifespan in fission yeast

Ohtsuka

Mita

Ogawa

et al. 2008

FEMS Yeast Research

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“…Escherichia coli -S. pombe shuttle vector pLBDblet (Yamada et al 1997) is a plasmid constructed by replacing the ura4 gene in the plasmid pDblet (Brun et al 1995) with Saccharomyces cerevisiae LEU2 gene. Plasmid pMN903 is a pLBDblet derivative that harbors 3.5 kb of HindIII fragment containing the catalase gene of S. pombe.…”

Section: Plasmidmentioning

confidence: 99%

The role of catalase in hydrogen peroxide resistance in fission yeastSchizosaccharomyces pombe

Mutoh

Nakagawa²,

Yamada³

1999

Can. J. Microbiol.

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The role of catalase in hydrogen peroxide resistance in Schizosaccharomyces pombe was investigated. A catalase gene disruptant completely lacking catalase activity is more sensitive to hydrogen peroxide than the parent strain. The mutant does not acquire hydrogen peroxide resistance by osmotic stress, a treatment that induces catalase activity in the wild-type cells. The growth rate of the disruptant is not different from that of the parent strain. Additionally, transformed cells that overexpress the catalase activity are more resistant to hydrogen peroxide than wildtype cells with normal catalase activity. These results indicate that the catalase of S. pombe plays an important role in resistance to high concentrations of hydrogen peroxide but offers little in the way of protection from the hydrogen peroxide generated in small amounts under normal growth conditions.

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“…During the course of our studies on the osmotic response in S. pombe (Aiba et al 1995;Ohmiya et al 1995Ohmiya et al , 1997Yamada et al 1997), we found that an atf1D mutant exhibits a novel phenotype: it shows an apparent hypersensitivity to calcium. In this study, we attempted to clarify this particular phenotype, with special reference to the MAPK signaling pathway discussed above.…”

Section: Introductionmentioning

confidence: 98%

Isolation of multicopy suppressors of the calcium sensitivity of a mutant lacking the bZIP transcription factor Atf1 in fission yeast

Ohmiya

Kato²,

Yamada³

et al. 1999

Mol Gen Genet

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In Schizosaccharomyces pombe, recent studies have uncovered a set of putative transcription factors of the basic leucine zipper (bZIP) type (e.g., Atf1, Pcr1, Pap1), which function downstream of the Sty1 mitogen-activated protein kinase (MAPK) cascade which is involved in stress-activated signal transduction. Accordingly, a delta atf1 mutant is known to exhibit osmosensitivity for growth, since one of the targets of Atf1 is the gpd1+ gene, which is responsible for the osmoadaptive glycerol production mediated by the Sty1 MAPK cascade. During the course of our studies on the osmotic response in S. pombe, we found that growth of a delta atf1 mutant is highly sensitive to the level of Ca2+ ions in the medium (but less sensitive to Mg2+ and Na+ ions). This phenotype seemed to be relevant to the osmosensitivity, because an delta gpd1 mutant showed a similar phenotype. An attempt was therefore made to isolate multicopy suppressors of the calcium sensitivity exhibited by the delta atf1 cells. Among such suppressors were several bZIP factors, including two known proteins (Atf21 and Pcr1), and two new ones (named Atf31 and Zip1). These factors were characterized further, in comparison to Atf1, with special reference to the Sty1 MAPK signaling pathway.

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Characterization of multicopy suppressor genes that complement a defect in the Wis1-Sty1 MAP kinase cascade involved in stress responses in Schizosaccharomyces pombe.

Cited by 11 publications

References 20 publications

A novel gene, ecl1+, extends the chronological lifespan in fission yeast

A novel gene, ecl1+, extends the chronological lifespan in fission yeast

The role of catalase in hydrogen peroxide resistance in fission yeastSchizosaccharomyces pombe

Isolation of multicopy suppressors of the calcium sensitivity of a mutant lacking the bZIP transcription factor Atf1 in fission yeast

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