Characterization of multidrug resistance in Vibrio species isolated from marine invertebrates from Andaman Sea

Abstract

This study describes the abundance of multidrug-resistant Vibrios associated with marine invertebrate hosts from the Andaman Sea, India. Thirty-eight Vibrio strains were isolated from surface mucus layers of coral Porites, Goniastrea, Pocillopora, Fungia, and eggs of spiny lobster (Panulirus penicillatus). Phenotypically, the majority of strains exhibited growth at a wide range of temperatures, salt tolerance, and diverse nutritional requirements. All the strains had more than 97% 16S rRNA sequence similarity with type species of the genus Vibrio where Vibrio fortis, and Vibrio alginolyticus were predominant. Multilocus Sequence Analysis (MLSA) using eight housekeeping genes namely ftsZ, gapA, gyrB, mreB, pyrH, recA, rpoA, and topA distributed the strains into 6 reported clades i.e., Harveyi, Ponticus, Nereis, Orientalis, Splendidus, and Mediterranei where nearly half of the total strains represented the clade Harveyi, followed by the clade Splendidus. Likewise, the PFGE profile indicated genomic heterogeneity among the strains resulting in their distribution in five major clusters. Resistance to different antimicrobials was tested following the disc diffusion method where all strains were found susceptible to chloramphenicol (30 µg) and resistant to streptomycin (10 µg), vancomycin (30 µg), sulfamethoxazole-trimethoprim (25 µg). Moreover, the resistant phenotype to other antimicrobials confirmed the abundance of multidrug resistance strains in this marine environment.

Physiology, metabolism, antibiotic resistance, and genetic diversity of Harveyi clade bacteria isolated from coastal mariculture system in China in the last two decades

“…Sequencing and purification of PCR products were performed by Shanghai Sangon Biological Engineering Technology and Services Co., Ltd. (Shanghai, China). The DNA sequences were analyzed using the BLAST tool of GenBank and optimized for highly similar sequences (Mega BLAST) (Kumari et al, 2020).…”

Identification of Vibrio ponticus as a bacterial pathogen of coral trout Plectropomus leopardus

“…The susceptibility of the pathogenic isolate to twenty-six antimicrobials was evaluated by the instruction of the manufacturer (Hangzhou Binhe Microorganism Reagent Co., Ltd., Hangzhou, China). The antibiotic susceptibilities of V. ponticus previously reported by Kim et al (2007); You (2018); Liu et al (2018) and Kumari et al (2020) were used as references.…”

Pufferfish (Tetraodon cutcutia) Sampled from a Freshwater River Serves as an Intermediate Reservoir of a Sucrose Nonfermenting Variant of Vibrio cholerae PS-4

“…Vibrios are ubiquitous and plentiful in aquatic environments, including estuaries, marine coastal waters, and sediments, and aquaculture practices worldwide ( 1 , 2 ). Several studies have demonstrated that vibrios are associated with aquatic animals ( 3 , 4 ). Due to rapid growth, salt tolerance, and biofilm-forming capacities, the genus Vibrio developed adaptive skills and the physiological flexibility to survive and flourish in the diverse oligotrophic environment ( 5 ).…”