Characterization of Multidrug-Resistant Biofilm-Producing <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> in Healthy Cattle and Cattle with Diarrhea

Bandyopadhyay, Samiran; Bhattacharyya, Debasish; Samanta, Indranil; Banerjee, Jaydeep; Habib,; Dutta, Tapan Kumar; Dutt, Triveni

doi:10.1089/mdr.2020.0298

Cited by 13 publications

(16 citation statements)

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“…Eighty-nine isolates from calf diarrhea, including 52 (81.3%) E. coli and 37 (90.2%) S. enterica , showed the MDR phenomenon. However, Bandyopadhyay et al [ 6 ] reported a lower MDR rate (12.3%) of E. coli strains isolated from calves with diarrhea than in the current study. Moreover, in line with the current study, Gebeyehu et al [ 32 ] from Ethiopia reported a 100.0% resistance rate to ampicillin and a 100.0% susceptibility rate to ciprofloxacin among Salmonella strains isolated from raw cow milk samples.…”

Section: Discussioncontrasting

confidence: 81%

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Intimin (eae) and virulence membrane protein pagC genes are associated with biofilm formation and multidrug resistance in Escherichia coli and Salmonella enterica isolates from calves with diarrhea

Hasson¹,

Judi

Salih

et al. 2022

BMC Res Notes

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Objectives This study aimed to evaluate the association of the intimin (eae) and pagC genes with biofilm formation and multidrug resistance (MDR) phenotype in Escherichia coli and Salmonella enterica collected from calves with diarrhea. Results Fecal samples (n: 150) were collected from calves with diarrhea. Of 150 fecal samples, 122 (81.3%) were culture positive and 115/122 (94.2%) were Gram-negative bacteria. Among them, E. coli (n = 64/115, 55.6%) was the most common isolate followed by S. enterica (n = 41/115, 35.6%). Also, 10 (8.6%) isolates were other Enterobacteriaceae bacteria including Klebsiella and Proteus species. Eighty-nine isolates (77.4%) from calf diarrhea, including 52 (81.3%) E. coli and 37 (90.2%) S. enterica were MDR. The eae and pagC genes were detected in 33 (51.5%) E. coli and 28 (68.3%) S. enterica isolates, respectively. There was a strong association between these genes and biofilm formation and MDR phenotype (P-value = 0.000). All E. coli isolates carrying the eae gene were biofilm producers and MDR. Also, all pagC-positive S. enterica isolates were MDR and 25 (89.3%) isolates of them produced biofilm.

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Section: Discussioncontrasting

confidence: 81%

“…Farm animals such as cattle are known reservoirs for multidrug resistance (MDR) bacteria such as E. coli [ 6 ]. The MDR phenomenon has increased worldwide and is considered a public health threat.…”

Section: Introductionmentioning

confidence: 99%

Intimin (eae) and virulence membrane protein pagC genes are associated with biofilm formation and multidrug resistance in Escherichia coli and Salmonella enterica isolates from calves with diarrhea

Hasson¹,

Judi

Salih

et al. 2022

BMC Res Notes

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“…Due to the extensive resistance in many of the Gramnegative bacteria, these pathogens are considered as a priority for R&D and antimicrobial drug discovery platforms, to facilitate the development of new antibiotics [43]. In addition, continuous monitoring of resistance rates in a given institution or geographical region is another necessity to successfully address AMR globally [44]. Bacteria may present with the MDR/XDR phenotype through the contribution of a variety of mechanisms, including both intrinsic and acquired resistance [45].…”

Section: Discussionmentioning

confidence: 99%

Interplay between Phenotypic Resistance to Relevant Antibiotics in Gram-Negative Urinary Pathogens: A Data-Driven Analysis of 10 Years’ Worth of Antibiogram Data

Gajdács

Bátori

Burián

2021

Life

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The global emergence of antimicrobial resistance (AMR) has become a critical issue for clinicians, as it puts the decades of developments in the medical field in jeopardy, by severely limiting the useful therapeutic arsenal of drugs, both in nosocomial and community-acquired infections. In the present study, a secondary analysis of taxonomic and resistance data was performed, corresponding to urinary tract infections (UTIs) caused by Gram-negative bacteria, detected between 1 January 2008 to 31 December 2017 at the Albert Szent-Györgyi Health Center, University of Szeged. The following were identifiable from the data collected: year of isolation; outpatient (OP)/inpatient (IP) origin of the isolate; taxonomy; and susceptibility/resistance to selected indicator antibiotics. Principal component analysis (PCA) and a correlation matrix were used to determine the association between the presences of resistance against indicator antibiotics in each taxonomic group. Overall, data from n = 16,240 outpatient and n = 13,964 inpatient Gram-negative UTI isolates were included in the data analyses. In E. coli, strong positive correlations were seen between resistance to ciprofloxacin (CIP) and gentamicin (GEN) resistance (OP: r = 0.6342, p = 0.049; IP: r = 0.9602, p < 0.001), whereas strong negative correlations were shown for fosfomycin (FOS) and nitrofurantoin (NIT) resistance (OP: r= −0.7183, p = 0.019; IP: r= −0.7437; p = 0.014). For Klebsiella spp. isolates, CIP resistance showed strong positive correlation with resistance to third-generation cephalosporins (3GC) and GEN (r = 0.7976, p = 0.006 and r = 0.7428, p = 0.014, respectively) in OP isolates, and with resistance to trimethoprim-sulfamethoxazole (SXT) and FOS (r = 0.8144, p = 0.004 and r = 0.7758, p < 0.001, respectively) in IP isolates. For members of the Citrobacter-Enterobacter-Serratia group, the resistance among indicator antibiotics showed a strong positive correlation, with the exception of FOS resistance. In the Proteus-Providencia-Morganella group, the strongest association was noted between CIP and SXT resistance (OP: r = 0.9251, p < 0.001; IP: r = 0.8007; p = 0.005). In the case of OP Acinetobacter spp., CIP showed strong and significant positive correlations with most indicator antibiotics, whereas for IP isolates, strong negative correlations arose among imipenem (IMI) resistance and resistance to other drugs. For Pseudomonas spp., strong and positive correlations were noted among resistance to β-lactam antibiotics and aminoglycosides, with the exception of ceftazidime (CEFT), showing strong, but negative correlations. Though molecular tests and sequencing-based platforms are now considered as the gold-standard for AMR surveillance, standardized collection of phenotypic resistance data and the introduction of Big Data analytic methods may be a viable alternative for molecular surveillance, especially in low-resource settings.

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“…Limited epidemiological studies in different parts of the world have revealed that goats, similar to other food animals, can act not only as a mere reservoir but also a potential spreader of the AMR pathogens or antibiotic resistance gene (ARG) through its milk and meat [ 4 , 5 ]. AMR pathogens such as ESBL and carbapenemase producers as well as methicillin-resistant and vancomycin-intermediate Staphylococcus aureus have been reported in different food and companion animals, including goats [ 6 , 7 , 8 ]. Altogether, the recent observations illustrate such a radical shift in perspective that goat farming can no longer be considered a farming system in isolation that presents no or minimum loads of AMR.…”

Section: Introductionmentioning

confidence: 99%

“…Altogether, the recent observations illustrate such a radical shift in perspective that goat farming can no longer be considered a farming system in isolation that presents no or minimum loads of AMR. Recently, we reported a higher proportion of resistant pathogens in cattle with diarrhoea than their healthy counterparts [ 8 ]. Diarrhoea, a multifactorial condition, is not uncommon in goats and often requires antimicrobial or antiparasitic intervention.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Resistance Pattern, Clustering Mechanisms and Correlation Matrix of Drug-Resistant Escherichia coli in Black Bengal Goats in West Bengal, India

et al. 2022

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A cross-sectional study covering four agro-climatic zones of West Bengal, India, was carried out to understand the risk-factors, antimicrobial resistance mechanism and clustering of the resistance characteristics of Escherichia coli isolated from healthy (170) and diarrhoeic (74) goats reared under intensive (52) and semi-intensive (192) farming practices. Of the 488 E. coli isolates, the majority, including the extended spectrum (n: 64, 13.11%) and AmpC β-lactamase (ACBL) (n: 86, 17.62%) producers, were resistant to tetracycline (25.2%), followed by enrofloxacin (24.5%), cefotaxime (21.5%) and amikacin (20.5%). Statistical modelling revealed that the isolates from diarrhoeic animals (p < 0.001) are likely to be more ACBL-positive than those from the healthy counterparts. Similarly, cefotaxime (p < 0.05) and enrofloxacin-resistance (p < 0.01) were significantly higher in diarrhoeic goats and in goats reared intensively. The isolates (n = 35) resistant to multiple drugs revealed the presence of β-lactamase [blaCTXM-1-(21), blaSHV-(7), blaTEM-(3), blaCMY-6-(1), blaCITM-(3)]; quinolone [qnrB-(10), qnrS-(7), aac(6’)-Ib-cr-(3)]; tetracycline [tetA-(19), tetB-(4)] and sulphonamide resistance determinants [sul1-(4)]; multiple plasmids, especially those belonging to the IncF and IncI1 replicon types; and active acrAB efflux pumps. Further, two isolates harbored the carbapenem resistance (blaNDM-5) gene and eight were strong biofilm producers. This first ever study conducted to unravel the status of AMR in goat farming reveals that not only the intensive farming practices but also certain clinical ailments such as diarrhoea can increase the shedding of the drug-resistant isolate. The emergence of multi-drug resistant (MDR) E. coli in goats, particularly those that are carbapenem resistant, is a cause for concern that indicates the spread of such pathogens even in the livestock sub-sector generally considered as naive.

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Characterization of Multidrug-Resistant Biofilm-Producing Escherichia coli and Klebsiella pneumoniae in Healthy Cattle and Cattle with Diarrhea

Abstract: This study describes comparative occurrence and characterization of multidrug-resistant (MDR) Escherichia coli and Klebsiella pneumoniae (KP) in healthy cattle (HC) and cattle with diarrhea (DC) in India.

Cited by 13 publications

References 74 publications

Intimin (eae) and virulence membrane protein pagC genes are associated with biofilm formation and multidrug resistance in Escherichia coli and Salmonella enterica isolates from calves with diarrhea

Intimin (eae) and virulence membrane protein pagC genes are associated with biofilm formation and multidrug resistance in Escherichia coli and Salmonella enterica isolates from calves with diarrhea

Interplay between Phenotypic Resistance to Relevant Antibiotics in Gram-Negative Urinary Pathogens: A Data-Driven Analysis of 10 Years’ Worth of Antibiogram Data

Antimicrobial Resistance Pattern, Clustering Mechanisms and Correlation Matrix of Drug-Resistant Escherichia coli in Black Bengal Goats in West Bengal, India

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