Characterization of Multifunctional Bacillus sp. GH1-13

Abstract

Several microorganisms in particular Bacillus subtilis group have been isolated from diverse places such as soils and the gastrointestinal tract of ruminants etc., and used as biocontrol agent against various plant pathogens and utilized as plant growth promoting agents. Among them, Bacillus is well known as one of the most useful bacteria for biocontrol and plant growth promotion. Bacterium GH1-13 was isolated from a reclaimed paddy field in Wando Island and identified as Bacillus velezensis using phylogenetic analysis on the basis of 16S rRNA and gyrB gene. It was confirmed that GH1-13 produced indole acetic acid (IAA) associated with promoted growth of rice root. GH1-13 showed characteristics of antagonization against the main pathogen of rice as well as diverse pathogenic fungi. GH1-13 had biosynthetic genes, bacillomycin, bacilycin, fengycin, iturin, and surfactin which are considered to be associated closely with inhibition of growth of pathogenic fungi and bacteria. This study showed that GH1-13 could be used as a multifunctional agent for biocontrol and growth promotion of crop.

Plant growth-promoting activity of beta-propeller protein YxaL secreted from Bacillus velezensis strain GH1-13

“…thaliana and rice seeds soaked in a solution containing YxaL in the sub-nanomolar range (0.1 ~ 1 mg L -1 ) showed a significant improvement with respect to root growth as did treatment with B . velezensis GH1-13 cells [9]. A previous study showed that the use of bovine serum albumin (BSA) as an organic N-source supplement produced similar effects on the root growth and development of A .…”

“…velezensis strain GH1-13 was isolated from rice paddy soil in Korea and can promote plant growth and suppress several pathogens [8]. This strain was shown to produce indole 3-acetic acid (IAA) associated with promoted growth of rice root [9]. The chromosome sequence of strain GH1-13 (GenBank accession number CP019040.1) contains highly homologous genes responsible for the biosynthesis of some plant hormones and secondary metabolites, such as IAA, 2,3-butanediol, non-ribosomal lipopeptides, and polyketide antimicrobials, which are believed to be more proficient for rhizosphere colonisation and pathogen control than other members of the B .…”

Enhanced Tolerance of Chinese Cabbage Seedlings Mediated by Bacillus aryabhattai H26-2 and B. siamensis H30-3 against High Temperature Stress and Fungal Infections

“…One μl of the bacterial suspension was subjected to a template for PCR. Primer pairs demonstrated in Kim et al (2016) for the six antimicrobial peptide biosynthetic genes were used. PCR amplifications were carried out in 50-μl reaction mixture.…”

Control Efficacy of Bacillus velezensis AFB2-2 against Potato Late Blight Caused by Phytophthora infestans in Organic Potato Cultivation

“…were obtained using options according to the Kimura twoparameter model (Kim et al, 2016;Kimura, 1980), and clustering was performed using the Neighbor-Join algorithms (Saitou and Nei, 1987) to a pairwise matrix, which was inferred by using the maximum composite likelihood method, with a 1,000-replicate (Felsenstein, 1985) bootstrap test using MEGA 7. The phylogenetic tree was drawn to scale, with the branch lengths measured by the number of substitutions per site.…”

“…A molecular phylogenetic tree based on the amino acid sequence of the 16S rRNA gene of the AFB2-2 strain was performed automatically using the MEGA version 7 ( Kumar et al, 2016 ) after multiple alignments of the data with the CLUSTAL W ( Thompson et al, 1994 ). Distances were obtained using options according to the Kimura two-parameter model ( Kim et al, 2016 ; Kimura, 1980 ), and clustering was performed using the Neighbor–Join algorithms ( Saitou and Nei, 1987 ) to a pairwise matrix, which was inferred by using the maximum composite likelihood method, with a 1,000-replicate ( Felsenstein, 1985 ) bootstrap test using MEGA 7. The phylogenetic tree was drawn to scale, with the branch lengths measured by the number of substitutions per site.…”