Characterization of murine antibody response to Salmonella typhimurium by a class-specific solid-phase radioimmunoassay

Metcalf, Eleanor S.; O'Brien, A D

doi:10.1128/iai.31.1.33-41.1981

Cited by 23 publications

(19 citation statements)

References 24 publications

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“…Consistent with previous studies (e.g. [18,19]), these results indicate that O-Ag, in the context of live bacteria, is a strong immunogen. Moreover, these results suggest that the epitopes of the majority of anti-O-Ag antibodies are a¡ected by acetylation.…”

Section: Polyclonal Antibody Response To Acetylated Versus Unacetylatsupporting

confidence: 92%

Effect of acetylation (O-factor 5) on the polyclonal antibody response to Salmonella typhimurium O-antigen

Kim¹

1999

FEMS Immunology and Medical Microbiology

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Antibodies directed against lipopolysaccharide (LPS) O-antigen are often critical in the immune response to Gram-negative pathogens. Mice were orally immunized with isogenic strains of Salmonella typhimurium that differ only in a minor modification of O-antigen, namely acetylation, mediated by the oafA locus. To specifically examine the effect of acetylation on the antibody response to O-antigen, antibody titers were determined against both acetylated and unacetylated LPS by ELISA. In mice immunized with an oafA+ strain, the median titer against acetylated LPS was 32-fold higher than the titer against unacetylated LPS. Mice immunized with the oafA- strain had an 8-fold higher titer against unacetylated LPS. Thus, acetylation of O-antigen alters recognition by the vast majority of individual antibodies. This differential antibody recognition of O-antigen had a statistically significant correlation with protection against subsequent challenge with virulent S. typhimurium.

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Section: Polyclonal Antibody Response To Acetylated Versus Unacetylatsupporting

confidence: 92%

Effect of acetylation (O-factor 5) on the polyclonal antibody response to Salmonella typhimurium O-antigen

Kim¹

1999

FEMS Immunology and Medical Microbiology

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“…3). This is explainable because in mice a significant amount of antibodies to Salmonella antigens were observed only around 2 weeks post-infection [25]. These results strongly suggest that antigen detection is a more reliable method than measuring the antibody levels for the early diagnosis of Salmonella infection.…”

Section: Discussionmentioning

confidence: 92%

Detection of porin antigen in serum for early diagnosis of mouse infections with Salmonella typhimurium

Muthukkumar

1992

FEMS Microbiology Letters

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The monoclonal antibodies to porin, an outer membrane protein isolated from Salmonella typhimurium and sandwich enzyme linked immunosorbent assay (ELISA) has made possible the detection of porin from sera of S. typhimurium‐infected mice. The specificity of the monoclonal antibodies was ascertained based on their cross‐reactivity with porins isolated from S. typhi, Shigella flexneri and Escherichia coli and lipopolysaccharide (LPS) of S. typhimurium and E. coli. Serum samples were found to be positive for porin as early as 3 days after intravenous and 5 days after oral infection. In addition, a positive correlation was observed between the bacterial load and the concentration of porin detected in the sera. On the other hand, analysis of sera for anti‐porin antibody showed diametrically opposite time kinetics with antigenaemia. These results indicate that porin accumulates in the serum of infected mice much earlier than the appearance of antibodies to porin. Thus detection of porin holds promise for early diagnosis of typhoid.

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“…Radioimmunoassay . The RIA of culture fluids (50 ul) for specific mouse anti-TML antibody on bacteria-coated microtiter plates was previously described (22) . TML' culture fluids were reanalyzed in microtiter wells coated with 50 Ad of purified TML-LPS diluted in phosphate buffer at a concentration of 100 ng LPS/well (23).…”

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confidence: 99%

Antibody-defective, genetically susceptible CBA/N mice have an altered Salmonella typhimurium-specific B cell repertoire.

Duran¹,

Metcalf²

1987

The Journal of Experimental Medicine

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CBA/N mice, which express the X-linked immunodeficiency gene xid, are susceptible to Salmonella typhimurium. The basis for this susceptibility is currently unknown. However, previous studies (10) from this laboratory have provided evidence that susceptibility may be due to a defective anti-S. typhimurium antibody response. In that report we hypothesized that the defective antibody response may be a reflection of an altered S. typhimurium-specific B cell repertoire. In the studies described here, we have investigated this hypothesis using a modification of the in vitro splenic focus system. The frequency and characteristics of salmonella-specific B cells in normal, innately resistant, CBA/Ca mice have been compared with those of salmonella-susceptible, anti-S. typhimurium antibody-defective CBA/N mice. The results show that CBA/N mice express no primary or secondary S. typhimurium-specific B cell precursors after stimulation with an acetone-killed and dried (AKD) preparation of S. typhimurium strain TML. However, after three immunizations, the CBA/N tertiary frequency of 15.4 per 10(6) splenic B cells was similar to the primary precursor frequency in immunologically normal CBA/Ca mice, but 23-fold lower than the tertiary precursor frequency in CBA/Ca control mice. Moreover, CBA/N mice had an altered isotype distribution pattern after stimulation with AKD-TML. Greater than 70% of the tertiary CBA/N TML-specific B cells secreted IgG2, in contrast to either nonimmune or primed control mice. In addition, 80% of the CBA/N TML-specific B cells secreted only a single isotype, whereas the majority of B cells from primed normal mice secreted multiple isotypes. Fine specificity analysis of the TML-specific B cells indicated that the array of antigenic determinants to which CBA/N B cells could respond was restricted. Although the majority of primed CBA/Ca and primed CBA/N B cells were specific for LPS, the fine specificity pattern exhibited by CBA/N B cells was similar to that observed in unprimed normal mice, i.e., the vast majority were specific for the O antigen region of the LPS molecule. In contrast, a major portion of the LPS-specific B cells in primed CBA/Ca mice were directed against the KDO/lipid A region of the LPS molecule. Therefore, it appears that CBA/N mice lack or are unable to stimulate the B cell subset that predominates in primed, normal mice. Taken together, these studies indicate that the basis for susceptibility of CBA/N mice to S. typhimurium is multifactorial and suggests that the inability of some animals to respond to some infectious agents may be related to holes in their B cell repertoire.

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Characterization of murine antibody response to Salmonella typhimurium by a class-specific solid-phase radioimmunoassay

Cited by 23 publications

References 24 publications

Effect of acetylation (O-factor 5) on the polyclonal antibody response to Salmonella typhimurium O-antigen

Effect of acetylation (O-factor 5) on the polyclonal antibody response to Salmonella typhimurium O-antigen

Detection of porin antigen in serum for early diagnosis of mouse infections with Salmonella typhimurium

Antibody-defective, genetically susceptible CBA/N mice have an altered Salmonella typhimurium-specific B cell repertoire.

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