Characterization of Muscarinic Receptors in Cultured Human Iris Sphincter and Ciliary Smooth Muscle Cells

WoldeMussie, Elizabeth; Feldmann, Barbara; Chen, June

doi:10.1006/exer.1993.1052

Cited by 46 publications

(32 citation statements)

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“…The messenger RNA of the m3 receptor was detected by Northern blot in the cultured human ciliary muscle cells and post mortem tissue (13,15). Furthermore, WoldeMussie et al (17) demonstrated that the muscarinic binding sites in these cells were most likely M3 receptors and, similar to our findings, the muscarinic agonist-induced PLC activation was mediated by an M3-like receptor. Interestingly, the m3/M3 receptor is also shown to be the most common muscarinic receptor subtype in the iris sphincter (14,17), implying that this receptor likely mediates the muscarinic agonist-induced miosis.…”

Section: Cell Contractionsupporting

confidence: 91%

“…Furthermore, WoldeMussie et al (17) demonstrated that the muscarinic binding sites in these cells were most likely M3 receptors and, similar to our findings, the muscarinic agonist-induced PLC activation was mediated by an M3-like receptor. Interestingly, the m3/M3 receptor is also shown to be the most common muscarinic receptor subtype in the iris sphincter (14,17), implying that this receptor likely mediates the muscarinic agonist-induced miosis. Indeed, Gabelt and Kaufman (16) receptor mediates muscarinic agonist-induced contractions of both the iris sphincter and the ciliary smooth muscles.…”

Section: Cell Contractionsupporting

confidence: 91%

“…They are named ml to m5, in which ml, m2 and m3 correspond to the Ml, M2 and M3 receptor subtypes that were defined by affinity profiles of selective antagonists. Since (13)(14)(15)(16)(17) . In this report we used the cultured human ciliary muscle cells and attempted to elucidate the muscarinic receptor subtype that mediates the carbachol-induced contraction of the muscle cells as well as the activation of phospholipase C (PLC).…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Muscarinic Receptor Involvement in Human Ciliary Muscle Cell Function

Pang¹,

Matsumoto²,

Tamm³

et al. 1994

Journal of Ocular Pharmacology and Therapeutics

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Section: Cell Contractionsupporting

confidence: 91%

Section: Cell Contractionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of Muscarinic Receptor Involvement in Human Ciliary Muscle Cell Function

Pang¹,

Matsumoto²,

Tamm³

et al. 1994

Journal of Ocular Pharmacology and Therapeutics

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“…After general evidence for muscarinic receptors [70,71], numerous work focused only on the subtype m3 [71][72][73][74][75], although the subtypes m1 and m2 were also detected [76]. A first localization study revealed that the muscarinic receptor subtypes m2 and m3 were predominantly in the inner portion, m5 predominantly in the outer portion of the ciliary muscle [77]; small amounts of the subtypes m1 and m4 were mentioned, and the presence of all subtypes m1-m5 with a dominance of m3 were confirmed [78].…”

Section: Membrane Elements and Receptorsmentioning

confidence: 99%

Morphologic characteristics of the human ciliary muscle

May¹

2017

New Front Ophthalmol

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The present review focusses on the morphological characteristics of the normal human ciliary muscle. It develops in the second and third trimenon during pregnancy but continues to change after birth with developing accommodation. The mature muscle forms three distinguishable portions which are connected but show individual characteristics. The outer portion origins and inserts with tendon-like structures, while the inner portion forms a sphincter-like appearance. The distinct different innervation is not yet completely described, nor exist portion-dependent summaries of the cytological characteristics. The specific intracellular, membraneous, and extracellular components described in the literature are listed and supplemented with own observations.

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“…M3 receptors mediate contractile responses in the iris sphincter of humans (22,23). The role of the M3 muscarinic receptor in controlling the contraction of the iris sphincter has been documented in M3 muscarinic receptor knockout mice (24,25).…”

Section: Physiology Of Muscarinic Receptorsmentioning

confidence: 99%

Dual Use Research of Concern: Derivatives of 3-Quinuclidinyl Benzilate (Bz)

Ball

2015

MMSL

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SummaryDual Use Research of Concern (DURC) deals with the unintended consequences of research and development and is particularly acute in the area of drug development. The United States National Science Advisory Board for Biosecurity (NSABB) has defined DURC as "research that, based on current understanding, can be reasonably anticipated to provide knowledge, products, or technologies that could be directly misapplied by others to pose a threat to public health, agriculture, plants, animals, the environment, or materiel" (1). One particular receptor antagonist and glycolate anticholinergic compound (BZ, QNB or 3-quinuclidinyl benzilate) was stockpiled by the United States as a non-lethal chemical weapon that incapacitates and severely degrades the capability of exposed individuals (2). Given time and medical treatment, combatants and non-combatants exposed to such incapacitating agents could recover without any long-term effects. The purpose of this review is to identify potential new and more potent incapacitating agents based on the structures of BZ or atropine using peer reviewed publications of new pharmaceutical agents. A number of peer reviewed studies have reported on compounds with effects observed at lower concentrations than or comparable to BZ suggesting that these compounds could also be developed as potential chemical incapacitating chemical warfare agents and represent good examples of the principal of Dual Use Research of Concern.

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Characterization of Muscarinic Receptors in Cultured Human Iris Sphincter and Ciliary Smooth Muscle Cells

Cited by 46 publications

References 0 publications

Characterization of Muscarinic Receptor Involvement in Human Ciliary Muscle Cell Function

Characterization of Muscarinic Receptor Involvement in Human Ciliary Muscle Cell Function

Morphologic characteristics of the human ciliary muscle

Dual Use Research of Concern: Derivatives of 3-Quinuclidinyl Benzilate (Bz)

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