Characterization of Musty Odor-Producing Actinomycetes from Tropics and Effects of Temperature on the Production of Musty Odor Compounds

Anuar, Nurul Syahirah Shamsol; Kassim, Aeyshah Abang; Utsumi, Motoo; Goto, Masafumi; Shimizu, Kazuya; Othman, Norhayani; Zakaria, Zuriati; Sugiura, Norío; Hara, Hirofumi

doi:10.1264/jsme2.me17109

Cited by 16 publications

(7 citation statements)

References 40 publications

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“…In this study, the detection of tpc gene in S10 was performed using a set of AmMIB primer, as reported by Auffret et al (2011), Park et al (2016 and Anuar et al (2017). However, no tpc gene was observed in S10 based on PCR analysis.…”

Section: Discussionmentioning

confidence: 93%

“…Musty odor compound production mostly occurs during the warmer season in temperate countries (Klausen et al, 2004). Therefore, it is believed that temperature is an important environmental factor that affects the metabolic activity of Streptomyces (Anuar et al, 2017). Moreover, the production of geosmin and 2-MIB is affected by several environmental factors, such as eutrophication, especially when nutrients exceed critical levels (nutrient enrichment) in lakes (Saadoun et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

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Limitation of nutrients stimulates musty odor production by <i>Streptomyces</i> sp. isolated from a tropical environment

Shudirman

Kassim

Anuar

et al. 2021

J. Gen. Appl. Microbiol.

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Musty odor production by actinomycetes is usually related to the presence of geosmin and 2methylisoborneol (2-MIB), which are synthesized by enzymes encoded by the geoA and tpc genes, respectively. Streptomyces spp. strain S10, which was isolated from a water reservoir in Malaysia, has the ability to produce geosmin when cultivated in a basal salt (BS) solid medium, but no 2-MIB production occurred during growth in BS medium. Strain S10 could produce higher levels of geosmin when the phosphate concentration was limited to 0.05 mg/L, with a yield of 17.53 ± 3.12 × × × × × 10 5 ng/L, compared with growth in BS medium. Interestingly, 2-MIB production was suddenly detected when the nitrate concentration was limited to 1.0 mg/L, with a yield of 1.4 ± 0.11 × × × × × 10 5 ng/L. Therefore, it was concluded that phosphate-and nitrate-limiting conditions could induce the initial production of geosmin and 2-MIB by strain S10. Furthermore, a positive amplicon of geoA was detected in strain S10, but no tpc amplicon was detected by PCR analysis. Draft genome sequence analysis showed that one open reading frame (ORF) contained a conserved motif of geosmin synthase with 95% identity with geoA in Streptomyces coelicolor A3 (2). In the case of the tpc genes, it was found that one ORF showed 23% identity to the known tpc gene in S. coelicolor A3(2), but strain S10 lacked one motif in the N-terminus.

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Section: Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Limitation of nutrients stimulates musty odor production by <i>Streptomyces</i> sp. isolated from a tropical environment

Shudirman

Kassim

Anuar

et al. 2021

J. Gen. Appl. Microbiol.

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“…Studies have indicated that geosmin and 2-methylisoborneol can be produced as secondary metabolites by microorganisms including actinomycetes, particularly Streptomyces [45], cyanobacteria [46], myxobacteria [47], fungi [48], and even by some plants [49]. Moreover, some studies have reported that actinomycetes play an important role in the life of ants and other insects [25,50].…”

Section: Streptomycetaceae and Nocardiaceae Are More Abundant In Soilmentioning

confidence: 99%

The nesting preference of an invasive ant is associated with the cues produced by actinobacteria in soil

et al. 2020

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Soil-dwelling animals are at risk of pathogen infection in soils. When choosing nesting sites, animals could reduce this risk by avoiding contact with pathogens, yet there is currently little evidence. We tested this hypothesis using Solenopsis invicta as a model system. Newly mated queens of S. invicta were found to nest preferentially in soil containing more actinobacteria of Streptomyces and Nocardiopsis and to be attracted to two volatiles produced by these bacteria, geosmin and 2-methylisoborneol. Actinobacteria-rich soil was favored by S. invicta and this soil contained fewer putative entomopathogenic fungi than adjacent areas. Queens in such soil benefited from a higher survival rate. In culture, isolated actinobacteria inhibited entomopathogenic fungi, suggested that their presence may reduce the risk of fungal infection. These results indicated a soil-dwelling ant may choose nest sites presenting relatively low pathogen risk by detecting the odors produced by bacteria with anti-fungal properties.

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“…However, there are reports that some organisms other than cyanobacteria may be the cause of odor compounds being generated when cyanobacteria do not appear (Parinet et al 2010;Cole & Williams 2011;Lee et al 2011). Recently, actinobacteria have been considered to be the causative organism of odor-producing compounds (Anuar et al 2017;Whangchai et al 2017). Since the pattern of increase and decrease in the amount of actinobacteria present in this study and the pattern of occurrence of odor compounds appeared very similar, it can be considered that actinobacteria may have had some influence on the occurrence of odor compounds in the Bukhan River and Paldang Lake.…”

Section: Correlation Between Odor Compounds In Water and Microorganismsmentioning

confidence: 99%

Study of the cause of the generation of odor compounds (geosmin and 2-methylisoborneol) in the Han River system, the drinking water source, Republic of Korea

et al. 2023

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Paldang Lake in the Han River system is an important source for supplying drinking water. Recently, the occurrence of odor compounds geosmin and 2-methylisoborneol (2-MIB), in Lake Paldang has adversely affected the drinking water supply. The causative organisms of odor compounds have not been identified. Thus, this study analyzed the correlation between odor compounds and microorganisms and identified the odor-producing microorganisms in the Han River system. Odor compounds were primarily detected in the Bukhan River system but not in the Namhan River system. Geosmin was detected at high concentrations in summer, whereas 2-MIB was detected at high concentrations in summer and autumn. Microorganisms that appeared at the time of geosmin occurrence were actinobacteria (0.317, p < 0.01) and Dolichospermum (0.326, p < 0.01) among cyanobacteria, and the correlation was significant. The occurrence of 2-MIB mainly coincided with actinobacteria (0.745, p < 0.01) and Pseudanabaena (0.321, p < 0.01) among cyanobacteria, and the correlation was significant. Therefore, to manage the odor compounds in Lake Paldang, which is a drinking water source, it is important to control the appearance of actinobacteria and cyanobacteria in the Bukhan River system.

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Characterization of Musty Odor-Producing Actinomycetes from Tropics and Effects of Temperature on the Production of Musty Odor Compounds

Cited by 16 publications

References 40 publications

Limitation of nutrients stimulates musty odor production by <i>Streptomyces</i> sp. isolated from a tropical environment

Limitation of nutrients stimulates musty odor production by <i>Streptomyces</i> sp. isolated from a tropical environment

The nesting preference of an invasive ant is associated with the cues produced by actinobacteria in soil

Study of the cause of the generation of odor compounds (geosmin and 2-methylisoborneol) in the Han River system, the drinking water source, Republic of Korea

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