Characterization of Mutations Disrupting Carotenoid Biosynthesis in Arabidopsis Thaliana

Pogson, Barry J.; Norris, Susan R.; McDonald, Kelly A.; Truong, Maria; DellaPenna, Dean

doi:10.1007/978-94-009-0173-5_712

Photosynthesis: From Light to Biosphere 1995

DOI: 10.1007/978-94-009-0173-5_712

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Characterization of Mutations Disrupting Carotenoid Biosynthesis in Arabidopsis Thaliana

Barry J. Pogson¹,

Susan R. Norris²,

Kelly A. McDonald³

et al.

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1998

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“…1 A shows the major intermediates and steps of the carotenoid biosynthetic pathway in higher plants and blocks in the pathway caused by the mutations lut1, lut2, and aba1. The level and composition of carotenoids in the single mutant lines were consistent with previous reports (12,40,41,45), as shown in Fig. 1B.…”

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confidence: 92%

Altered xanthophyll compositions adversely affect chlorophyll accumulation and nonphotochemical quenching in Arabidopsis mutants

Pogson

Niyogi

Björkman

et al. 1998

Proc. Natl. Acad. Sci. U.S.A.

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298

257

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Collectively, the xanthophyll class of carotenoids perform a variety of critical roles in light harvesting antenna assembly and function. The xanthophyll composition of higher plant photosystems (lutein, violaxanthin, and neoxanthin) is remarkably conserved, suggesting important functional roles for each. We have taken a molecular genetic approach in Arabidopsis toward defining the respective roles of individual xanthophylls in vivo by using a series of mutant lines that selectively eliminate and substitute a range of xanthophylls. The mutations, lut1 and lut2 (lut ‫؍‬ lutein deficient), disrupt lutein biosynthesis. In lut2, lutein is replaced mainly by a stoichiometric increase in violaxanthin and antheraxanthin. A third mutant, aba1, accumulates normal levels of lutein and substitutes zeaxanthin for violaxanthin and neoxanthin. The lut2aba1 double mutant completely lacks lutein, violaxanthin, and neoxanthin and instead accumulates zeaxanthin. All mutants were viable in soil and had chlorophyll a͞b ratios ranging from 2.9 to 3.5 and near wild-type rates of photosynthesis. However, mutants accumulating zeaxanthin exhibited a delayed greening virescent phenotype, which was most severe and often lethal when zeaxanthin was the only xanthophyll present. Chlorophyll f luorescence quenching kinetics indicated that both zeaxanthin and lutein contribute to nonphotochemical quenching; specifically, lutein contributes, directly or indirectly, to the rapid rise of nonphotochemical quenching. The results suggest that the normal complement of xanthophylls, while not essential, is required for optimal assembly and function of the light harvesting antenna in higher plants.

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supporting

confidence: 92%

Altered xanthophyll compositions adversely affect chlorophyll accumulation and nonphotochemical quenching in Arabidopsis mutants

Pogson

Niyogi

Björkman

et al. 1998

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

298

257

View full text Add to dashboard Cite

show abstract

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Characterization of Mutations Disrupting Carotenoid Biosynthesis in Arabidopsis Thaliana

Cited by 1 publication

References 9 publications

Altered xanthophyll compositions adversely affect chlorophyll accumulation and nonphotochemical quenching in Arabidopsis mutants

Altered xanthophyll compositions adversely affect chlorophyll accumulation and nonphotochemical quenching in Arabidopsis mutants

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