Characterization of mycelia from wood-decay species by TGA and IR spectroscopy

Girometta, Carolina Elena; Dondi, Daniele; Baiguera, Rebecca Michela; Bracco, Francesco; Branciforti, Diego Savio; Buratti, Simone; Lazzaroni, Simone; Savino, Elena

doi:10.1007/s10570-020-03208-4

Cited by 34 publications

(29 citation statements)

References 58 publications

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“…The p ~1530 cm −1 is mainly due to the aromatic rings conjugated with C=C, N-H from seco amines and amides and C-O bending. As mentioned in other studies, this region important to observe chitin and ergosterol [46,47]. The region between the peaks of and ~1230 cm −1 is affected by polysaccharides from C-O bonds from different so amines, and amides, C-N from chitin, and also O-H bonds.…”

Section: Ftir-atr Analysis Of Myceliummentioning

confidence: 58%

“…Absorbance reaches its highest values in the region between 1200 and 800 cm −1 . This region joins the signals from the vibrations of C-O (C-OH included) found in carbohydrates, C-N and C-C [46]. Lastly, the region 800-400 cm −1 is less informative, with hardly distinguishable bands.…”

Section: Ftir-atr Analysis Of Myceliummentioning

confidence: 87%

“…The region 3600-3000 cm −1 includes the signals from the vibrations of O-H and N-H bonds by carboxyl, hydroxyl and phenol groups with H-bonds, amines, and amides. The second region (3000-2800 cm −1 ) is mainly attributed to C-H bonds in alkane backbone and aldehydes [46]. The region 1800-800 cm −1 contains more specific information on the chemical composition of the mycelium, where it was possible to observe differences in its spectra in both conditions and in all species analyzed, with the exception of A. affinis.…”

Section: Ftir-atr Analysis Of Myceliummentioning

confidence: 98%

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Unveiling Biological Activities of Marine Fungi: The Effect of Sea Salt

et al. 2021

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There is an urgent need for new substances to overcome current challenges in the health sciences. Marine fungi are known producers of numerous compounds, but the manipulation of growth conditions for optimal compound production can be laborious and time-consuming. In Portugal, despite its very long coastline, there are only a few studies on marine fungi. From a collection of Portuguese marine fungi, we screened for antimicrobial, antioxidant, enzymatic, and cytotoxic activities. Mycelia aqueous extracts, obtained by high pressure-assisted extraction, and methanolic extracts of culture media showed high antioxidant, antimicrobial, and cytotoxic activities. The mycelium extracts of Cladosporium rubrum showed higher antioxidant potential compared to extracts from other fungi. Mycelia and culture media extracts of Aspergillus affinis and Penicillium lusitanum inhibited the growth of Staphylococcus aureus, Kocuria rhizophila, Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa, including multiresistant strains. Penicillium lusitanum and Trichoderma aestuarinum inhibited the growth of clinical strains of Candida albicans, C. glabrata, C. parapsilosis, and C. tropicalis. All extracts from culture media were cytotoxic to Vero cells. Sea salt induced alterations in the mycelium’s chemical composition, leading to different activity profiles.

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Section: Ftir-atr Analysis Of Myceliummentioning

confidence: 58%

Section: Ftir-atr Analysis Of Myceliummentioning

confidence: 87%

Section: Ftir-atr Analysis Of Myceliummentioning

confidence: 98%

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Unveiling Biological Activities of Marine Fungi: The Effect of Sea Salt

et al. 2021

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“…3 c) of the self-grown materials reveals how hydroxyl, carboxyl and amide groups are present in both strains since polysaccharides, lipids and proteins compose the fungal cell wall (Fig. 1 a) 71 – 73 . The main differences in the ATR-FTIR spectra between the two strains under study concern the area corresponding to the amide bond stretching.…”

Section: Resultsmentioning

confidence: 99%

Advanced mycelium materials as potential self-growing biomedical scaffolds

Antinori

Contardi

Suarato

et al. 2021

Sci Rep

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Mycelia, the vegetative part of fungi, are emerging as the avant-garde generation of natural, sustainable, and biodegradable materials for a wide range of applications. They are constituted of a self-growing and interconnected fibrous network of elongated cells, and their chemical and physical properties can be adjusted depending on the conditions of growth and the substrate they are fed upon. So far, only extracts and derivatives from mycelia have been evaluated and tested for biomedical applications. In this study, the entire fibrous structures of mycelia of the edible fungi Pleurotus ostreatus and Ganoderma lucidum are presented as self-growing bio-composites that mimic the extracellular matrix of human body tissues, ideal as tissue engineering bio-scaffolds. To this purpose, the two mycelial strains are inactivated by autoclaving after growth, and their morphology, cell wall chemical composition, and hydrodynamical and mechanical features are studied. Finally, their biocompatibility and direct interaction with primary human dermal fibroblasts are investigated. The findings demonstrate the potentiality of mycelia as all-natural and low-cost bio-scaffolds, alternative to the tissue engineering systems currently in place.

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“…Several protocols using FTIR spectroscopy have been described to identify moulds [19,20,[22][23][24][25]. Only a few studies have used FTIR to identify wood-decay fungi, but none to identity S. lacrymans [26,27]. In this study, we used different fungal growth conditions and different mycelial harvesting methods to select the best protocol for the identification of S. lacrymans by Attenuated total reflectance (ATR)-FTIR spectroscopy.…”

Section: Introductionmentioning

confidence: 99%

FTIR Spectroscopy for Identification and Intra-Species Characterization of Serpula lacrymans

2021

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The dry rot fungus Serpula lacrymans is the most destructive fungal agent of wood building materials in Europe, Russia, North America, and Japan. The identification of this wood-deteriorating agent and the discrimination of different fungal isolates is very important for the control of buildings in general and for the preservation of cultural heritage in particular. The objective of the study was to develop a Fourier transform infrared (FTIR) method coupled with a partial least square discriminant analysis (PLS-DA) for the sample preparation and identification of S. lacrymans. Five distinct S. lacrymans strains were analysed and compared to two strains of unrelated fungal species. Different methods of mycelial growth, sample preparation, and FTIR spectral data normalisation were compared. FTIR analysis of a harvested mycelium grown on the surface of a polyether sulfone microfiltration membrane deposited on a malt extract agar medium, followed by vector normalization and PLS-DA statistical analysis, resulted in 100% correct attribution at phylum, species, and strain level, regardless of the type of standardization used. This study confirms the applicability of FTIR spectroscopy for the identification of S. lacrymans and the discrimination of different strains belonging to this species.

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Characterization of mycelia from wood-decay species by TGA and IR spectroscopy

Cited by 34 publications

References 58 publications

Unveiling Biological Activities of Marine Fungi: The Effect of Sea Salt

Unveiling Biological Activities of Marine Fungi: The Effect of Sea Salt

Advanced mycelium materials as potential self-growing biomedical scaffolds

FTIR Spectroscopy for Identification and Intra-Species Characterization of Serpula lacrymans

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