Characterization of Mycosphaerellaceae species associated with citrus greasy spot in Panama and Spain

Aguilera‐Cogley, Vidal; Berbegal, Mónica; Catalá, Santiago; Brentu, F. C.; Armengol, Josep; Civera, Antonio Vicent

doi:10.1371/journal.pone.0189585

Cited by 16 publications

(18 citation statements)

References 23 publications

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“…This consortium was chosen because (1) it could be successfully revived from either glycerol or lyophilized stocks stored at −80° C, (2) it had a relatively low complexity with 35 bacterial OTUs that were detected by 16S amplicon sequencing (3) it had a higher diversity and evenness at the phylum level compared to the other six consortia and (4) it contained OTUs of interest that were found in the source soil, including representatives matching taxa known to respond to or degrade chitin such as Rhodococcus ( Sun et al, 2014 ) and Streptomyces ( Schrempf, 1999 , 2001 ). We also detected fungal species within this consortium using ITS sequencing: one unknown fungal species as well as a species in the family Mycosphaerellaceae ( Supplementary Table S3 ), a family of fungi that is known to have a number of interactions with plant species ( Aguilera-Cogley et al, 2017 ). Importantly, a second round of storage and revival (Generation 2) had little impact on the makeup of this consortium ( Figure 5B and Supplementary Figure S7 ).…”

Section: Resultsmentioning

confidence: 99%

Development and Analysis of a Stable, Reduced Complexity Model Soil Microbiome

et al. 2020

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The soil microbiome is central to the cycling of carbon and other nutrients and to the promotion of plant growth. Despite its importance, analysis of the soil microbiome is difficult due to its sheer complexity, with thousands of interacting species. Here, we reduced this complexity by developing model soil microbial consortia that are simpler and more amenable to experimental analysis but still represent important microbial functions of the native soil ecosystem. Samples were collected from an arid grassland soil and microbial communities (consisting mainly of bacterial species) were enriched on agar plates containing chitin as the main carbon source. Chitin was chosen because it is an abundant carbon and nitrogen polymer in soil that often requires the coordinated action of several microorganisms for complete metabolic degradation. Several soil consortia were derived that had tractable richness (30-50 OTUs) with diverse phyla representative of the native soil, including Actinobacteria, Bacteroidetes, Firmicutes, Proteobacteria, and Verrucomicrobia. The resulting consortia could be stored as glycerol or lyophilized stocks at −80 • C and revived while retaining community composition, greatly increasing their use as tools for the research community at large. One of the consortia that was particularly stable was chosen as a model soil consortium (MSC-1) for further analysis. MSC-1 species interactions were studied using both pairwise co-cultivation in liquid media and during growth in soil under several perturbations. Co-abundance analyses highlighted interspecies interactions and helped to define keystone species, including Mycobacterium, Rhodococcus, and Rhizobiales taxa. These experiments demonstrate the success of an approach based on naturally enriching a community of interacting species that can be stored, revived, and shared. The knowledge gained from querying these communities and their interactions will enable better understanding of the soil microbiome and the roles these interactions play in this environment.

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Section: Resultsmentioning

confidence: 99%

Development and Analysis of a Stable, Reduced Complexity Model Soil Microbiome

et al. 2020

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“…The fungal species which are most closely related to Magnuscella marinae belong to phylum Ascomycota, class Dothideomycetes, order Capnodiales, and family either Teratosphaeria (such as genus Eupenidiella and Hortaea ) [30, 54] or Mycosphaerellaceae (such as genus Stenella ) [55]. These two families are generally characterized by their widespread presence as saprophytes, opportunistic human pathogens, and phytopathogens [51, 54].…”

Section: Resultsmentioning

confidence: 99%

Characterization of a Halotolerant Fungus from a Marine Sponge

Anteneh

Brown

Franco

2019

BioMed Research International

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Introduction Marine sponges have established symbiotic interactions with a large number of microorganisms including fungi. Most of the studies so far have focussed on the characterization of sponge-associated bacteria and archaea with only a few reports on sponge-associated fungi. During the isolation and characterization of bacteria from marine sponges of South Australia, we observed multiple types of fungi. One isolate in particular was selected for further investigation due to its unusually large size and being chromogenic. Here, we report on the investigations on the physical, morphological, chemical, and genotypic properties of this yeast-like fungus. Methods and Materials Sponge samples were collected from South Australian marine environments, and microbes were isolated using different isolation media under various incubation conditions. Microbial isolates were identified on the basis of morphology, staining characteristics, and their 16S rRNA or ITS/28S rRNA gene sequences. Results Twelve types of yeast and fungal isolates were detected together with other bacteria and one of these fungi measured up to 35 μm in diameter with a unique chromogen compared to other fungi. Depending on the medium type, this unique fungal isolate appeared as yeast-like fungi with different morphological forms. The isolate can ferment and assimilate nearly all of the tested carbohydrates. Furthermore, it tolerated a high concentration of salt (up to 25%) and a range of pH and temperature. ITS and 28S rRNA gene sequencing revealed a sequence similarity of 93% and 98%, respectively, with the closest genera of Eupenidiella, Hortaea, and Stenella. Conclusions On the basis of its peculiar morphology, size, and genetic data, this yeast-like fungus possibly constitutes a new genus and the name Magnuscella marinae, gen nov., sp. nov., is proposed. This study is the first of its kind for the complete characterization of a yeast-like fungus from marine sponges. This novel isolate developed a symbiotic interaction with living hosts, which was not observed with other reported closest genera (they exist in a saprophytic relationship). The observed unique size and morphology may favour this new isolate to establish symbiotic interactions with living hosts.

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“…When these fungi are cultured in vitro , the pigment produced within their mycelia varies according to growth substrate, and might be used as a diagnostic trait and/or may provide additional information regarding phylogenetically related fungi (Saparrat et al., 2009; Kowalski et al., 2016). Aguilera-Cogley et al. (2017) raised more experimental data about limitations of the use of colour of fungal colonies growing on agar cultures and their ability to synthesize pigments as criteria for species identification in Mycosphaerellaceae.…”

Section: Introductionmentioning

confidence: 99%

Identification of an intermediate for 1,8-dihydroxynaphthalene-melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum)

Medina

Lucentini

Franco

et al. 2018

Heliyon

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Fulvia fulva (syn. Cladosporium fulvum, Mycosphaerellaceae) is a dematiaceous fungus that causes tomato leaf mould. It is characterized by its biotrophic lifestyle and the synthesis of the bianthraquinone secondary metabolite, cladofulvin. The aim of the study was to characterize the dark pigment photochemically synthesized by F. fulva and to elucidate its biochemical pathway. We isolated a black pigment from in vitro cultures of the fungus. We determined the pigment to be 1,8-dihydroxynaphthalene (DHN)-melanin based on its chemical and photochemical characteristics, as well as the presence of flaviolin, when fungal reductases were inhibited by tricyclazole. Furthermore, the pks1 gene involved in pigment synthesis has a KS domain already associated with DHN-melanin. Our findings support the relevance of studying melanization in F. fulva.

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Characterization of Mycosphaerellaceae species associated with citrus greasy spot in Panama and Spain

Cited by 16 publications

References 23 publications

Development and Analysis of a Stable, Reduced Complexity Model Soil Microbiome

Development and Analysis of a Stable, Reduced Complexity Model Soil Microbiome

Characterization of a Halotolerant Fungus from a Marine Sponge

Identification of an intermediate for 1,8-dihydroxynaphthalene-melanin synthesis in a race-2 isolate of Fulvia fulva (syn. Cladosporium fulvum)

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