Characterization of myeloperoxidase and its contribution to antimicrobial effect on extracellular traps in flounder (Paralichthys olivaceus)

Gan, Qiujie; Chi, Heng; Dalmo, Roy Ambli; Meng, Xianghu; Tang, Xiaoqian; Xing, Jing; Sheng, Xiuzhen; Zhan, Wenbin

doi:10.3389/fimmu.2023.1124813

Cited by 8 publications

(2 citation statements)

References 64 publications

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“…V. anguillarum strain had been previously isolated from diseased flounder and was stored in our laboratory [18]. Inactivated V. anguillarum was prepared as previously described [13].…”

Section: Inactivated Bacteria Immunization and Sample Collectionmentioning

confidence: 99%

Transcriptome Analysis of Peritoneal Cells Reveals the Early Immune Response of Flounder (Paralichthys olivaceus) to Inactivated Vibrio anguillarum Immunization

Meng,

Chi,

Zhang

et al. 2023

Vaccines

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Vibrio anguillarum (V. anguillarum) is a bacterium that seriously harms flounder and other aquaculture species. Vaccination is an effective means of preventing vibriosis and is mainly administered by intraperitoneal injection. Effective antigen processing at the initial stage of immunization is essential to elicit adaptive immune responses and improve vaccine efficacy. To understand the early immune response of flounder caused by inactivated V. anguillarum, we detected the transcriptome profiles of the cells in the peritoneal cavity (PoPerCs) after inactivated V. anguillarum immunization. More than 10 billion high-quality reads were obtained, of which about 89.33% were successfully mapped to the reference genome of flounder. A total of 1985, 3072, 4001, and 5476 differentially expressed genes were captured at 6, 12, 24, and 48 h post immunization, respectively. The hub module correlated with the immunization time was identified by WGCNA. GO and KEGG analysis showed that hub module genes were abundantly expressed in various immune-related aspects, including the response to stimuli, the immune system process, signal transducer activity, autophagy, the NOD-like receptor signaling pathway, the toll-like receptor signaling pathway, the T cell receptor signaling pathway, and Th17 cell differentiation. Additionally, genes related to Th cell differentiation are presented as heatmaps. These genes constitute a complex immune regulatory network, mainly involved in pathogen recognition, antigen processing and presentation, and Th cell differentiation. The results of this study provide the first transcriptome profile of PoPerCs associated with inactivated V. anguillarum immunity and lay a solid foundation for further studies on effective V. anguillarum vaccines.

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“…V. anguillarum strain had been previously isolated from diseased flounder and was stored in our laboratory [18]. Inactivated V. anguillarum was prepared as previously described [13].…”

Section: Inactivated Bacteria Immunization and Sample Collectionmentioning

confidence: 99%

Transcriptome Analysis of Peritoneal Cells Reveals the Early Immune Response of Flounder (Paralichthys olivaceus) to Inactivated Vibrio anguillarum Immunization

Meng,

Chi,

Zhang

et al. 2023

Vaccines

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“…In contrast, EPO, LPO, and TPO contain only two ester bonds between conserved aspartate and glutamate in their sequence alignment ( Figure 1 ) [ 2 , 3 , 4 ]. HMPO is found in neutrophils and plays a role in innate immunity [ 5 , 6 ]. It is involved in the host defense mechanism to fight microbicidal attacks [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

A Bacterial Myeloperoxidase with Antimicrobial Properties

Céré

Delord

Ymbe

et al. 2023

BioTech

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The four mammalian peroxidases (myeloperoxidase, eosinophilperoxidase, lactoperoxidase, and thyroid peroxidase) are widely studied in the literature. They catalyze the formation of antimicrobial compounds and participate in innate immunity. Owing to their properties, they are used in many biomedical, biotechnological, and agro-food applications. We decided to look for an enzyme that is easiest to produce and much more stable at 37 °C than mammalian peroxidases. To address this question, a peroxidase from Rhodopirellula baltica, identified by bioinformatics tools, was fully characterized in this study. In particular, a production and purification protocol including the study of heme reconstitution was developed. Several activity tests were also performed to validate the hypothesis that this peroxidase is a new homolog of mammalian myeloperoxidase. It has the same substrate specificities as the human one and accepts I−, SCN−, Br−, and Cl− as (pseudo-) halides. It also exhibits other auxiliary activities such as catalase and classical peroxidase activities, and it is very stable at 37 °C. Finally, this bacterial myeloperoxidase can kill the Escherichia coli strain ATCC25922, which is usually used to perform antibiograms.

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Immunostimulatory and antagonistic potential of the methanolic extract of Oedogonium intermedium SCB in Cirrhinus reba challenged with Aeromonas hydrophila

Chattaraj,

Das Mohapatra

2023

Aquacult Int

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Characterization of myeloperoxidase and its contribution to antimicrobial effect on extracellular traps in flounder (Paralichthys olivaceus)

Cited by 8 publications

References 64 publications

Transcriptome Analysis of Peritoneal Cells Reveals the Early Immune Response of Flounder (Paralichthys olivaceus) to Inactivated Vibrio anguillarum Immunization

Transcriptome Analysis of Peritoneal Cells Reveals the Early Immune Response of Flounder (Paralichthys olivaceus) to Inactivated Vibrio anguillarum Immunization

A Bacterial Myeloperoxidase with Antimicrobial Properties

Immunostimulatory and antagonistic potential of the methanolic extract of Oedogonium intermedium SCB in Cirrhinus reba challenged with Aeromonas hydrophila

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