Characterization of N-ethylmaleimide-sensitive proton pump in the rat kidney. Localization along the nephron.

Ait-Mohamed, A K; Marsy, S.; Barlet, C.; Khadouri, C.; Doucet, Alain

doi:10.1016/s0021-9258(18)67119-4

Cited by 49 publications

(7 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mitochondrial H + -ATPase has the highest sensitivity to DCCD (0.1-0.5 mol l Ϫ1 ), followed by vacuolar H + -ATPase (1-10 mol l Ϫ1 ) and then plasma membrane H + -ATPase (10-100 mol l Ϫ1 ). The dose-response curves of the ATPase activity in gill tissue for NEM and DCCD are very similar to those reported for rat kidney H + -ATPase (Ait-Mohamed et al 1986). DES and PCMBS were also reported to inactivate the F o moiety of H + -ATPase (Pedersen and Carafoli, 1987).…”

Section: Discussionsupporting

confidence: 79%

“…1B). The sensitivity profiles of fish gill ATPase towards both NEM and DCCD are similar to those found for the inhibition of H + -ATPase of rat kidney (Ait-Mohamed et al 1986).…”

Section: Resultssupporting

confidence: 71%

“…30% of the ATPase activity was NEM-insensitive and is of unknown origin. Unidentified NEM-insensitive ATPase has also been detected in mammalian kidney (Ait-Mohamed et al 1986;Garg and Narang, 1988). Bornancin et al (1980) presented evidence of Cl Ϫ /HCO 3 Ϫ -ATPase in gill plasma membrane of rainbow trout.…”

Section: Discussionmentioning

confidence: 97%

“…Urinary acidification by turtle bladder (Arruda et al 1981) and proton transport across freshwater frog skin (Ehrenfeld et al 1985), both mediated by H + -ATPase, are also vanadate-sensitive. However, vanadate fails to inhibit NEM-sensitive ATPase activity and proton transport in mammalian kidney (Gluck and Al-Awqati, 1984;Ait-Mohamed et al 1986), which were believed to be mediated by vacuolar H + -ATPase (Forgac, 1989).…”

Section: Discussionmentioning

confidence: 99%

“…The existence of the H + -ATPase is well documented not only in freshwater frog skin, which has the same Na + uptake function as freshwater fish gills, but also in other tight epithelia, such as turtle urinary bladder (Steinmetz and Andersen, 1982) and mammalian renal collecting tubule (Gluck and Al-Awqati, 1984;Ait-Mohamed et al 1986). Nethymaleimide, a covalent SH-reactive compound, is an H + -ATPase inhibitor commonly used to identify H + -ATPases in different organisms (Pedersen and Carafoli, 1987).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

H+ -ATPase Activity in Crude Homogenates of Fish Gill Tissue: Inhibitor Sensitivity and Environmental and Hormonal Regulation

Lin

Randall

1993

Journal of Experimental Biology

187

View full text Add to dashboard Cite

N-ethymaleimide-sensitive ATPase activity was measured in crude homogenates of gill tissue from rainbow trout using a coupled-enzyme ATPase assay in the presence of EGTA, ouabain and azide. This NEM-sensitive ATPase activity, determined to be about 1.5 micromole mg-1 protein h-1 at 15 °C for freshwater trout, is also inhibited by other H+-ATPase blockers such as DCCD, DES, PCMBS and bafilomycin. It is concluded, therefore, that the NEM-sensitive ATPase activity was generated by a proton-translocating ATPase. Since this NEM-sensitive ATPase was also sensitive to the plasma membrane ATPase inhibitor vanadate, we conclude that the H+-ATPase in fish gill is of the plasma membrane type. The major role of the H+-ATPase in the gill epithelium is to facilitate Na+ uptake from fresh water. Sodium concentration in the external medium was the primary regulator of the H+-ATPase in fish gills, with low sodium levels being associated with high H+-ATPase activity. High external calcium concentration had a marked stimulatory effect on H+-ATPase activity in fish gills when the sodium level was low. Environmental hypercapnia induced a 70 % increase in the H+-ATPase activity in fish gills. H+-ATPase activity was also elevated in freshwater fish after chronic cortisol infusion.

show abstract

Section: Discussionsupporting

confidence: 79%

“…1B). The sensitivity profiles of fish gill ATPase towards both NEM and DCCD are similar to those found for the inhibition of H + -ATPase of rat kidney (Ait-Mohamed et al 1986).…”

Section: Resultssupporting

confidence: 71%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

H+ -ATPase Activity in Crude Homogenates of Fish Gill Tissue: Inhibitor Sensitivity and Environmental and Hormonal Regulation

Lin

Randall

1993

Journal of Experimental Biology

187

View full text Add to dashboard Cite

show abstract

Structural Correlates of Transport in Distal Tubule and Collecting Duct Segments

Wade

Brown

1992

Comprehensive Physiology

View full text Add to dashboard Cite

The sections in this article are: Structural Approaches Light and Electron Microscopy Electron Microscopy of Freeze‐Fractured and Freeze‐Dried Tissue Enzyme Localization Lectin and Antibody Localization Strategies Quantitative Evaluation of Tubular Structures Overview of Specialized Cell Types Distal Convoluted Tubule Cells Connecting Tubule Cells Principal Cells Intercalated Cells Structural Changes Associated with Transport Regulation Structural Correlates of Sodium and Potassium Transport Structural Correlates of ADH Action Intercalated Cells

show abstract

Biochemical Characterization of Individual Nephron Segments

Guder

Morel

1992

Comprehensive Physiology

View full text Add to dashboard Cite

The sections in this article are: Biochemical Basis of Active Tubular Transport Biochemical Mechanisms of Hormone Action Cyclic AMP as Intracellular Messenger of Hormones Inositol Polyphosphates, Diacylglycerol, and Ca 2+ as Hormone Messengers Insulin and Growth Factors Action of Steroid Hormones Including Vitamin D Hormone Thyroid Hormones Biochemical Functions of the Proximal Tubule Coupling of Metabolism and Transport Brush‐Border Enzymes Renal Gluconeogenesis Amino Acid and Peptide Metabolism Biotransformation Reactions Thin Limbs of Henle's Loop Thick Ascending Limb of Henle's Loop Tamm‐Horsfall Glycoprotein The Distal Convoluted Tubule The Collecting Tubule Metabolism and Transport ATPases Organic Osmolytes

show abstract

Characterization of N-ethylmaleimide-sensitive proton pump in the rat kidney. Localization along the nephron.

Cited by 49 publications

References 34 publications

H+ -ATPase Activity in Crude Homogenates of Fish Gill Tissue: Inhibitor Sensitivity and Environmental and Hormonal Regulation

H+ -ATPase Activity in Crude Homogenates of Fish Gill Tissue: Inhibitor Sensitivity and Environmental and Hormonal Regulation

Structural Correlates of Transport in Distal Tubule and Collecting Duct Segments

Biochemical Characterization of Individual Nephron Segments

Contact Info

Product

Resources

About