Characterization of NaCl tolerance in <i>Desulfovibrio vulgaris</i> Hildenborough through experimental evolution

Zhou, Aifen; Baidoo, Edward E. K.; He, Zhili; Mukhopadhyay, Aindrila; Baumohl, Jason K.; Benke, Peter I.; Joachimiak, Marcin P.; Xie, Mao; Song, Rong; Arkin, Adam P.; Hazen, Terry C.; Keasling, Jay D.; Wall, Judy D.; Stahl, David A.; Zhou, Jizhong

doi:10.1038/ismej.2013.60

Cited by 55 publications

(74 citation statements)

References 43 publications

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“…Six populations (EC1 to EC6, non-stress-evolved) were cultured in standard growth condition (defined medium LS4D with 60 mM lactate as electron donor and 50 mM sulfate as electron acceptor) and six populations (ES7 to ES12, stress evolved) were cultured in salt stress condition (LS4D+100 mM NaCl) (Zhou et al, 2013) for 1200 generations. Briefly, the cultures were maintained at 37°C and serially transferred every 48 h with a 1-to-100 dilution (10 ml culture volume in 18 × 150 mm anaerobic culture tubes).…”

Section: Bacteria Strains and Growth Conditionsmentioning

confidence: 99%

“…Growth rate, yield and lag phase values were obtained independently from the growth curve of each replicate (Zhou et al, 2013). The changes of growth rate, yield and lag phase along the evolution were obtained by dividing the measurements in evolved populations by the measurements in the ancestral population.…”

Section: Salt Tolerance Phenotype Testmentioning

confidence: 99%

“…Dramatically improved salt tolerance has been observed in stress-evolved strain ES9-11 compared with the non-stress-evolved strain EC3-10 and ancestral DvH (Zhou et al, 2013). As a first step in identifying the genetic bases of salt adaptation, the genomes of ES9-11, EC3-10 and the ancestral DvH were sequenced.…”

Section: Mutations In Evolved Dvh Strainsmentioning

confidence: 99%

“…In contrast, frequencies of DVU1204b and DVU0597b were about 0.5 in EC3. Due to the relatively high Na + concentration (~210 mM) in LS4D (the evolution condition for EC3), and improved salt tolerance in EC3-10 and the best growth performances of EC3 among EC populations (Zhou et al, 2013), mutations in DVU2287, DVU2571, DVU1204 and DVU0597 might also contribute to salt tolerance.…”

Section: Dynamics Of Mutation Frequencies Over Evolutionmentioning

confidence: 99%

“…Taking advantages of the available knowledge, we initiated an experimental evolution of DvH under salt stress to investigate the molecular mechanisms of evolutionary adaptation to salt stress. Analysis of the representative evolved strains demonstrated significant changes in salt tolerance via gene transcription, metabolite abundances (for example, organic solutes) and cellular composition (for example, phospholipid fatty acids) (Zhou et al, 2013), but the genetic basis of salt adaptation remains elusive (that is, evolution).…”

Section: Introductionmentioning

confidence: 99%

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Rapid selective sweep of pre-existing polymorphisms and slow fixation of new mutations in experimental evolution of Desulfovibrio vulgaris

Zhou

Hillesland

et al. 2015

The ISME Journal

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To investigate the genetic basis of microbial evolutionary adaptation to salt (NaCl) stress, populations of Desulfovibrio vulgaris Hildenborough (DvH), a sulfate-reducing bacterium important for the biogeochemical cycling of sulfur, carbon and nitrogen, and potentially the bioremediation of toxic heavy metals and radionuclides, were propagated under salt stress or non-stress conditions for 1200 generations. Whole-genome sequencing revealed 11 mutations in salt stress-evolved clone ES9-11 and 14 mutations in non-stress-evolved clone EC3-10. Whole-population sequencing data suggested the rapid selective sweep of the pre-existing polymorphisms under salt stress within the first 100 generations and the slow fixation of new mutations. Population genotyping data demonstrated that the rapid selective sweep of pre-existing polymorphisms was common in salt stress-evolved populations. In contrast, the selection of pre-existing polymorphisms was largely random in EC populations. Consistently, at 100 generations, stress-evolved population ES9 showed improved salt tolerance, namely increased growth rate (2.0-fold), higher biomass yield (1.8-fold) and shorter lag phase (0.7-fold) under higher salinity conditions. The beneficial nature of several mutations was confirmed by site-directed mutagenesis. All four tested mutations contributed to the shortened lag phases under higher salinity condition. In particular, compared with the salt tolerance improvement in ES9-11, a mutation in a histidine kinase protein gene lytS contributed 27% of the growth rate increase and 23% of the biomass yield increase while a mutation in hypothetical gene DVU2472 contributed 24% of the biomass yield increase. Our results suggested that a few beneficial mutations could lead to dramatic improvements in salt tolerance.

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Section: Bacteria Strains and Growth Conditionsmentioning

confidence: 99%

Section: Salt Tolerance Phenotype Testmentioning

confidence: 99%

Section: Mutations In Evolved Dvh Strainsmentioning

confidence: 99%

Section: Dynamics Of Mutation Frequencies Over Evolutionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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