Characterization of nanozyme kinetics for highly sensitive detection

Panferov, Vasily G.; Wang, Xiaoqin; Liu, Juewen

doi:10.1039/d4an00250d

Cited by 6 publications

(3 citation statements)

References 28 publications

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“…In contrast to HRP, many nanozymes require much higher concentrations of H2O2 for optimal performance (Panferov et al, 2024). Under these conditions, there is a significant likelihood of non-specific TMB oxidation due to the presence of trace transition metal cations.…”

Section: The Effect Of Chelatormentioning

confidence: 99%

“…As was recently pointed out by Panferov et al many modern nanozyme-based colorimetric immunoassays utilize non-optimal concentrations of both substrates, TMB and H2O2 (Panferov et al, 2024). Nanozymes have lower affinity towards H2O2 but can operate at its high concentrations, therefore increase of H2O2 improves detection limit.…”

Section: Optimizing Tmb and H2o2 Concentrations And Determining Optim...mentioning

confidence: 99%

“…Only in a limited number of papers optimization of substrate composition took place, e.g. papers (Chen et al, 2021;Hormozi Jangi et al, 2020;Panferov et al, 2024;Sun et al, 2016).…”

mentioning

confidence: 99%

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Enhancing Nanozyme-Based Colorimetric Assays by Optimizing Substrate Composition

Khramtsov,

Valeev,

Eremina

et al. 2024

Preprint

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Nanozymes, while promising alternatives to natural peroxidases in colorimetric assays, are often hindered by lower catalytic efficiencies. While various strategies exist to enhance signal intensity in nanozyme-based assays, substrate optimization remains largely underexplored. The vast majority of studies rely on standard sodium acetate buffers or commercially-sourced substrates optimized for horseradish peroxidase, neglecting the unique catalytic properties of different nanozymes. This work presents a systematic optimization of 3,3',5,5'-tetramethylbenzidine (TMB)-based substrate compositions for four common nanozymes: iron oxide, LaNiO3, Mn-doped CeO2, and platinum nanoparticles. Our findings reveal that while sodium acetate buffer is suitable for LaNiO3, alternative buffers significantly enhance signal intensity (41-68%) for the other nanozymes. Further optimization of ionic strength, organic co-solvent type and concentration, and TMB/H2O2 concentrations yielded improvements in signal intensity, analytical sensitivity, and assay time. This study also identifies common pitfalls encountered during substrate optimization and proposes potential solutions. We posit that substrate composition should be a standard optimization step in the development of nanozyme-based assays, and the use of commercially-sourced substrates with undisclosed compositions should be avoided.

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Section: The Effect Of Chelatormentioning

confidence: 99%

Section: Optimizing Tmb and H2o2 Concentrations And Determining Optim...mentioning

confidence: 99%

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Enhancing Nanozyme-Based Colorimetric Assays by Optimizing Substrate Composition

Khramtsov,

Valeev,

Eremina

et al. 2024

Preprint

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Nanozymes for Treating Ocular Diseases

Wong,

Liu

2024

Adv Healthcare Materials

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Nanozymes, characterized by their nanoscale size and enzyme‐like catalytic activities, exhibit diverse therapeutic potentials, including anti‐oxidative, anti‐inflammatory, anti‐microbial, and anti‐angiogenic effects. These properties make them highly valuable in nanomedicine, particularly ocular therapy, bypassing the need for systemic delivery. Nanozymes show significant promise in tackling multi‐factored ocular diseases, particularly those influenced by oxidation and inflammation, like dry eye disease, and age‐related macular degeneration. Their small size, coupled with their ease of modification and integration into soft materials, facilitates the effective penetration of ocular barriers, thereby enabling targeted or prolonged therapy within the eye. This review is dedicated to exploring ocular diseases that are intricately linked to oxidation and inflammation, shedding light on the role of nanozymes in managing these conditions. Additionally, recent studies elucidating advanced applications of nanozymes in ocular therapeutics, along with their integration with soft materials for disease management, are discussed. Finally, this review outlines directions for future investigations aimed at bridging the gap between nanozyme research and clinical applications.This article is protected by copyright. All rights reserved

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Optical and Catalytic Properties of Nanozymes for Colorimetric Biosensors: Advantages, Limitations, and Perspectives

Panferov,

Liu

2024

Advanced Optical Materials

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Detection of colorimetric signals is commonly used in various analytical methods and for testing in non‐laboratory and resource‐limited settings. The performance of colorimetric assays is largely based on nanoparticles and their unique optical properties. Multifunctional nanoparticles combining optical and enzyme‐like catalytic properties—known as nanozymes—hold great promise for analytical applications as signal‐generating labels. However, the extensive focus on the catalytic properties leaves their unique optical properties overlooked. In this article, the use of the optical and catalytic properties of nanozymes is reviewed for analytical applications relying on the inherent optical properties of nanozymes, the colorimetric detection of a catalytically‐formed product, and colorimetric changes of nanoparticles caused by the catalytically‐formed product. The impact of the extinction coefficient of nanozymes and reaction products, as well as the kinetic parameters of nanozymes on the sensitivity and limit of detection of assays, are quantitatively evaluated. Finally, the existing limitations and prospects of nanozymes for colorimetric biosensors are summarized.

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Characterization of nanozyme kinetics for highly sensitive detection

Abstract: Enzymes (1) and nanozymes (2) have significant differences in the Michaelis–Menten constant. Detection of nanozymes using enzyme substrates results in lower catalytic activity and a significantly higher limit of detection.

Cited by 6 publications

References 28 publications

Enhancing Nanozyme-Based Colorimetric Assays by Optimizing Substrate Composition

Enhancing Nanozyme-Based Colorimetric Assays by Optimizing Substrate Composition

Nanozymes for Treating Ocular Diseases

Optical and Catalytic Properties of Nanozymes for Colorimetric Biosensors: Advantages, Limitations, and Perspectives

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