Characterization of Necrotoxigenic<i>Escherichia coli</i>(NTEC) Strains Isolated from Healthy Calves in Poland

Osek, Jacek

doi:10.1111/j.1439-0450.2001.00481.x

Cited by 5 publications

(5 citation statements)

References 32 publications

(43 reference statements)

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“…In this study, E. coli K99 was detected as not only 13.41% in the diarrhoeic calves but also 5.55% in the healthy calves by ELISA. Osek (2001) described that E. coli F17 was detected as 26.6% in the healthy calves by PCR. In this study, we found that while E. coli K99 in 11 faeces samples of diarrhoeic calves were positive by antigen ELISA, E. coli K99 in seven faeces samples of diarrhoeic calves were positive by PCR.…”

Section: Discussionmentioning

confidence: 99%

The Studies on the Aetiology of Diarrhoea in Neonatal Calves and Determination of Virulence Gene Markers ofEscherichia coliStrains by Multiplex PCR

Güler

Turgut

et al. 2009

Zoonoses and Public Health

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The purpose of this study was to determine aetiological agents of diarrhoea in neonatal calves and to investigate virulence gene markers of Escherichia coli strains isolated from calves by multiplex polymerase chain reaction (PCR). Eighty-two diarrhoeic calves and 18 healthy calves were used as subjects. Faeces were taken from the rectums of all the calves and were subjected to bacterial culture. Antigen enzyme-linked immunosorbent assay (ELISA) was performed to detect rotavirus, coronavirus and E. coli K99 in faeces of all the calves. A multiplex PCR was used to characterize E. coli strains in all the calves. Escherichia coli was isolated from 37 faeces samples, Enterococcus ssp. was isolated from 22 faeces samples and Salmonella was isolated from one faeces sample in diarrhoeic calves. Furthermore, only E. coli was isolated from all 18 faeces samples of healthy calves. Of the 37 E. coli isolated from diarrhoeic calves, K99 (18.9%), F41 (18.9%), heat-stable enterotoxin a (STa) (18.9%), Shiga toxin 1 (Stx1; 13.5%) and Shiga toxin 2 (Stx2; 5.4%) and intimin (8.1%) genes were identified by multiplex PCR. Of the 18 E. coli isolated from healthy calves, K99 (16.6%) and intimin (55.5%) genes were identified by PCR. A total of 15 rotavirus, 11 coronavirus and 11 E. coli K99 were detected in diarrhoeic calves by the antigen ELISA. As a result, this study shows that rotavirus, coronavirus, E. coli and Enterococcus ssp. were determined to play a role in the aetiology of diarrhoea in the neonatal calves. K99, F41, STa, Stx1 and Stx2 were found as the most common virulence gene markers of E. coli strains isolated from calves with diarrhoea. Multiplex PCR may be useful for characterization of E. coli isolated from calves.

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Section: Discussionmentioning

confidence: 99%

The Studies on the Aetiology of Diarrhoea in Neonatal Calves and Determination of Virulence Gene Markers ofEscherichia coliStrains by Multiplex PCR

Güler

Turgut

et al. 2009

Zoonoses and Public Health

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“…The necrotoxigenic E. coli (NTEC) produces a cytolethal distending toxin (Cdt) and two cytotoxic necrotizing factors CNF1 and CNF2. The CNF1 and CNF2 induce multi-nucleation and necrosis of eukaryotic cells [10]. Oral administration of NTEC in neonatal animals causes septicemia and enteritis [11].…”

Section: Introductionmentioning

confidence: 99%

“…Rate of change in virulence factors can serve as an alert about the endemic state of the pathogen with subsequent recommendation for trade limitation from the affected locality. The EPEC, STEC, and NTEC are opportunistic pathogens, which have also been isolated from non-diarrheic calves [10,14,15]. Understanding risk factors, e.g., farm size, age, sex, season, concurrent disease, colostrum management, calving factors, perinatal treatments, housing, feeding, genetics, and environment is very important in controlling E. coli infections and improving the health and performance of calves [16,17,18].…”

Section: Introductionmentioning

confidence: 99%

Virulence factors and antibiograms of Escherichia coli isolated from diarrheic calves of Egyptian cattle and water buffaloes

et al. 2020

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Diarrhea caused by Escherichia coli in calves is an important problem in terms of survivability, productivity and treatment costs. In this study, 88 of 150 diarrheic animals tested positive for E. coli. Of these, 54 samples had mixed infection with other bacterial and/or parasitic agents. There are several diarrheagenic E. coli pathotypes including enteropathogenic E. coli (EPEC), Shiga-toxin producing E. coli (STEC), enterotoxigenic E. coli (ETEC) and necrotoxigenic E. coli (NTEC). Molecular detection of virulence factors Stx2,

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“…This strain is one of the most significant E. coli involved in the etiology of infectious calf diarrhea. In contrast, another fimbrial type of E. coli isolated from our material (E. coli F17) is not considered an important cause of diarrhea despite its high prevalence in calves (Osek 2001, Foster and Smith 2009, Herrera-Luna et al 2009). The infection with ETECs leads to secretory diarrhea with resultant loss of water and electrolytes.…”

Section: Discussionmentioning

confidence: 70%

Acid-base disorders in calves with chronic diarrhea

Bednarski¹,

Kupczyński²,

Sobiech³

2015

Polish Journal of Veterinary Sciences

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The aim of this study was to analyze disorders of acid-base balance in calves with chronic diarrhea caused by mixed, viral, bacterial and Cryptosporydium parvum infection. We compared results obtained with the classic model (Henderson-Hasselbalch) and strong ion approach (the Steward model). The study included 36 calves aged between 14 and 21 days. The calves were allocated to three groups: I -(control) non-diarrheic calves, group II -animals with compensated acid-base imbalance and group III calves with compensated acid-base disorders and hypoalbuminemia. Plasma concentrations of Na, P, albumin and lactate were measured. In the classic model, acid-base balance was determined on the basis of blood pH, pCO 2 , HCO 3 -, BE and anion gap. In the strong ion model, strong ion difference (SID), effective strong anion difference, total plasma concentration of nonvolatile buffers (A Tot ) and strong ion gap (SIG) were measured.The control calves and the animals from groups II and III did not differ significantly in terms of their blood pH. The plasma concentration of HCO 3 -, BE and partial pressure of CO2 in animals from the two groups with chronic diarrhea were significantly higher than those found in the controls. The highest BE (6.03 mmol/l) was documented in calves from group II. The animals from this group presented compensation resulted from activation of metabolic mechanisms. The calves with hypoalbuminemia (group III) showed lower plasma concentrations of albumin (15.37 g/L), Cl -(74.94 mmol/L), Mg 2+ (0.53 mmol/L), P (1.41 mmol/L) and higher value of anion gap (39.03 mmol/L). This group III presented significantly higher SID 3 (71.89 mmol/L), SID 7 (72.92 mmol/L) and SIG (43.53 mmol/L) values than animals from the remaining groups (P<0.01), whereas A Tot (6.82 mmol/L) were significantly lower. The main finding of the correlation study was the excellent relationship between the AG corr and SID 3 , SID 7 , SIG. In conclusion, chronic diarrhea leads to numerous water-electrolyte disorders. Characterization of acid-base disturbance in these cases suggests that classic model have some limitations. This model can not be recommended for use whenever serum albumin or phosphate concentrations are markedly abnormal.

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Characterization of NecrotoxigenicEscherichia coli(NTEC) Strains Isolated from Healthy Calves in Poland

Cited by 5 publications

References 32 publications

The Studies on the Aetiology of Diarrhoea in Neonatal Calves and Determination of Virulence Gene Markers ofEscherichia coliStrains by Multiplex PCR

The Studies on the Aetiology of Diarrhoea in Neonatal Calves and Determination of Virulence Gene Markers ofEscherichia coliStrains by Multiplex PCR

Virulence factors and antibiograms of Escherichia coli isolated from diarrheic calves of Egyptian cattle and water buffaloes

Acid-base disorders in calves with chronic diarrhea

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