Characterization of new biosurfactant produced by Trichosporon montevideense CLOA 72 isolated from dairy industry effluents

Abstract

The yeast strain CLOA 72 isolated from the effluent of a dairy industry in Brazil and identified as Trichosporon montevideense, was able to grow and produce a glycolipid biosurfactant when cultured on a mineral medium (MM) with sunflower oil as the carbon source. Biosurfactant production was partially growth-associated and maximal emulsification activity was observed at 144 h of cultivation (78.92%). The biosurfactant purified by precipitation with ethanol showed 78.66% emulsifying activity when used in concentrations above 4.5 mg/ml and was able to reduce the surface tension of water to values below 44.9 mN/m. The critical micellar concentration (CMC) was found to be 2.2 mg/ml. The highest emulsifying activity (E(24)) has been observed with vegetable oils, toluene, kerosene, isooctane, cyclohexane, hexane, diesel oil and hexadecane as compared to mineral oil and oleic acid. The biosurfactant also showed good stability during exposure to 100 degrees C for different periods of time (10 to 60 min), to high salinity (30% of NaCl, KCl and NaHCO(3)), and to a wide range of pH values (1-10). The biosurfactant purified by gel filtration chromatography is a glycolipid, with lipid portion containing 16.03% (9Z)-octadec-9-enoic acid, 14.92% hexadecanoic acid, and 9.63% (E) octadec-9-enoic acid and the carbohydrate portion containing mannose (35.29%), xylose (41.99%), arabinose (17.47%), and glucose (5.25%).

Advances in utilization of renewable substrates for biosurfactant production

“…These characteristics indicated that rhamnolipids were potential candidate for use in bioremediation of hydrocarbon-contaminated sites or in the petroleum industry a conclusion that was also confirmed by (Perfumo et al 2010). (Monteiro et al 2009) reported the growth and biosurfactant production using sunflower oil supplemented mineral medium by the yeast Trichosporon montevideense , CLOA 72. The glycolipid produced exhibited good surface and emulsifying activity with vegetable oils, toluene, kerosene, isooctane, cyclohexane, hexane, diesel oil and hexadecane.…”

Novel rhamnolipid biosurfactants produced by a polycyclic aromatic hydrocarbon-degrading bacterium Pseudomonas aeruginosa strain NY3

“…They are primarily produced by fermentation with renewable carbon sources, such as vegetable oils (Costa et al, 2006). Some cheaper raw materials and recycling substrates from agricultural operations, food processing and other industrial processes could be used for cost-effective biosurfactant production (Makkar and Cameotra, 2002; Nitschke et al, 2005, 2009; Monteiro et al, 2009). Their environmental compatibility, effectiveness at extremes of temperature, pH and salinity, and high specificity to targeted pathogens (Haba et al, 2000; Rivardo et al, 2009) make these biosurfactants attractive and desirable for widespread application.…”

Bioactivity of the compounds isolated from Blepharocalyx salicifolius

“…The compound 2, (2E)-1-(4´-hydroxy-2´,6´-dimethoxy-3´,5´-dimethylphenyl)-3-phenylprop-2-en-1-one, showed similar structure to 1, seems to be an unedited substance. The compounds 3-4, isolated as pale yellow solids, were elucidated by means of NMR data, hydrolysis, peracetylation and GC/MS analysis (Cota et al, 2008;Monteiro et al,2009;Monteiro et al, 2010). It was possible to confirm the identity of the sugar moiety as rhamnose for 3 (Figure 1) and arabinose for 4 (Figure 2).…”